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Nerve grafting object containing orientation nanofiber nerve tract and preparing method of nerve grafting object

A nanofiber and nerve transplantation technology, applied in medical science, textile and papermaking, prosthesis, etc., can solve the problem of low probability of contact between damaged nerves and guiding fibers, unstable nerve graft preparation process, and difficulty in surgical suture technology. It is easy to guide the growth of injured nerves, easy to suture in surgery, and the effect of enhanced practicability.

Inactive Publication Date: 2014-03-19
NANTONG TEXTILE VOCATIONAL TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The traditional nerve graft preparation process is unstable, and the size of the graft cannot be adjusted, making it difficult to meet clinical needs;
[0007] 2. Due to the limitations of electrospinning, its spinning rate is low, which is not conducive to the industrialization of preparation technology. If the nanofibers are collected directly through the rotating roller to form a tubular scaffold, the production speed is 8-10h / piece, and the fiber orientation Perpendicular to the axial direction of the tubular stent, it is difficult to guide nerve growth;
[0008] 3. The guiding performance needs to be improved, the contact probability between the damaged nerve and the guiding fiber is not high, which is not conducive to guiding the growth of the damaged nerve, and the surgical suturing technique is difficult and impractical

Method used

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  • Nerve grafting object containing orientation nanofiber nerve tract and preparing method of nerve grafting object
  • Nerve grafting object containing orientation nanofiber nerve tract and preparing method of nerve grafting object
  • Nerve grafting object containing orientation nanofiber nerve tract and preparing method of nerve grafting object

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preparation example Construction

[0027] The preparation method of this specific embodiment is:

[0028] (1) Select natural high polymers and synthetic high polymers according to needs, and dissolve them in volatile solvents, use electrospinning method, use high-speed rollers to collect, obtain nano-scale fibers arranged in parallel, and fiber orientation Consistent with the direction of rotation of the roller. The diameter of the rotating roller is 200-300mm, and the rotating speed is 0-4000rpm.

[0029] (2) Remove the fiber film on the rotating roller, cut the fiber film into 3-15mm strips perpendicular to the fiber orientation, and then wrap it on a metal rotating roller with a diameter of 0.1mm. The fiber orientation and the rotating roller axis In parallel, a fiber bundle with a diameter of 2-5mm is formed, which serves as the "core" A of the nerve graft.

[0030] (3) Near the two ends of the fiber bundle, put cylinders with the same diameter as the fiber bundle on the rotating rollers respectively, wit...

Embodiment 1

[0037] 1. Dissolving polyglycolic acid (PGA) in a mixed solvent of chloroform and acetone (2:1 by volume) to obtain a spinning solution with a concentration of 8%. The electrospinning process conditions are: voltage 20KV, distance 11cm, flow rate 3ml / h, collected by a rotating roller, the rotating speed of the rotating roller is 3000rpm, the diameter of the rotating roller is 200mm, electrospinning for 10h, forming an oriented fiber with a width of 10cm and a thickness of 35um membrane.

[0038] 2. Cut the fiber film into 3mm strips perpendicular to the fiber arrangement direction, and wrap them on a rotating roller with a diameter of 0.1mm to form a fiber bundle with a length of 3mm and a diameter of 3mm.

[0039] 3. At both ends of the fiber bundle, put a cylinder with a diameter and a length of 3 mm.

[0040] 4. Cut the fiber membrane into 9mm strips perpendicular to the fiber arrangement direction, and wrap it on the surface of the cylinder and the fiber bundle, wrapping ...

Embodiment 2

[0044] 1. Dissolve PLGA (LA / GA=65:35) in chloroform / N, N-dimethylformamide (DMF) (8:2 by volume) mixed solvent to obtain a spinning solution with a concentration of 15% . The electrospinning process conditions are: voltage 18KV, distance 11cm, flow rate 2ml / h, collected by a rotating roller, the rotating speed of the rotating roller is 1700rpm, the diameter of the rotating roller is 250mm, electrospinning for 10h, forming an oriented fiber with a width of 10cm and a thickness of 38um membrane.

[0045] 2. Cut the fiber film into 5mm strips perpendicular to the direction of fiber arrangement, and wrap them on a rotating roller with a diameter of 0.1mm to form a fiber bundle with a length of 5mm and a diameter of 4mm.

[0046] 3. At both ends of the fiber bundle, put a cylinder with a diameter and a length of 4 mm.

[0047] 4. Cut the fiber membrane into 13mm strips perpendicular to the fiber arrangement direction, and wrap them on the surface of the cylinder and the fiber bun...

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Abstract

The invention relates to the biomedical field of high molecular materials, in particular to a nerve grafting object containing an orientation nanofiber nerve tract and a preparing method of the nerve grafting object. A core (A) composed of the nanofiber tract is placed in the middle of the nerve grafting object, a sheath (B) composed of an irregular fiber layer (B2) and an orientation layer (B1) is arranged on the outer face of the nerve grafting object, the diameter of the core (A) composed of the nanofiber tract is 2-5mm, the length of the core (A) composed of the nanofiber tract is 3-15mm, the fiber diameter of the nanofiber tract is 40-300nm, the length of the sheath (B) is 9-25mm, the orientation layer (B1) comprises parallel-arrayed fibers of 100-500nm, and the irregular fiber layer (B2) comprises disorder-arrayed fibers of 100-500nm. The technology is stable, grafting object size is adjustable, the clinic requirement is met, production efficiency is improved, industrialization is convenient, guiding performance is high, using is convenient, and practicability is improved.

Description

Technical field: [0001] The invention relates to the biomedical field of polymer materials, in particular to a nerve graft containing oriented nanofiber nerve bundles and a preparation method thereof. Background technique: [0002] After a nerve ruptures, all signals reaching the nerve endings are lost. A transected nerve will have difficulty regenerating without surgical reconnection. As a result, tens of thousands of peripheral nerves require surgical repair each year. If the nerve is cut directly, surgical suturing can be used to reconnect the "end to end" and restore nerve function. However, in most cases, for long-distance nerve defects, if only relying on surgical suturing, it is easy to generate tension between the broken ends, and it is difficult to achieve the desired therapeutic effect. In this case, only nerve grafts can be selected to "bridge" the nerve stumps, guide the regenerated axons to pass through the damaged area, and finally achieve the recovery of ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61L27/40D04H1/74D04H1/728
Inventor 尹桂波陈和春陈桂香郭立强
Owner NANTONG TEXTILE VOCATIONAL TECH COLLEGE
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