Method for producing candida for feeds by fermenting high-salt-content amino acid wastewater
A Candida and amino acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable treatment of amino acid wastewater, high cost of concentration treatment process, high investment and operation costs, and achieve nitrogen The effect of high resource utilization efficiency, high application and promotion potential, and strong competitive advantage
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Embodiment 1
[0025] Example 1: Acclimatization and cultivation of Candida strains for feed.
[0026] 1. Take the waste liquid from an amino acid factory in Wuhan, after the hydrolysis of hair acid to produce cysteine, and use it as the high-salt amino acid wastewater required below. Its composition table is as follows:
[0027] Table 1 Contents of related elements in amino acid wastewater
[0028] project name
unit
Test results
Method basis
Total Nitrogen (N)
%
8.79
GB / T8572-2010
Sodium (Na)
%
0.52
ICP method
Chlorine (Cl)
g / L
131.26
AgCl nephelometric method
Lead (Pb)
mg / kg
0.91
ICP method
Arsenic (As)
mg / kg
0.076
Mercury (Hg)
mg / kg
0.014
Chromium (Cr)
mg / kg
1.51
ICP method
Cadmium (Cd)
mg / kg
0.06
ICP method
[0029] Table 2 Amino acid composition and content of amino a...
Embodiment 2
[0037] Fermentation of Candida utilis in liquid medium with glucose as carbon source and waste water with different concentrations of amino acids as nitrogen source.
[0038] 1. Prepare two kinds of liquid fermentation medium for high salinity amino acid wastewater with different concentrations. Liquid fermentation media with high salinity amino acid wastewater concentration of 165g / L and 330g / L were prepared respectively, wherein the high salinity amino acid wastewater was the only nitrogen source (its source and composition were consistent with Example 1), and the liquid fermentation culture The base also adds other chemical substrates required by microorganisms, and its components are: glucose: 45 and 90g / L, KH 2 PO 4 : 7.5 and 15g / L, yeast powder: 5 and 10g / L, MgSO 4 : 4.5 and 9g / L, CaCO 3 : 1 and 2g / L, FeSO 4 : 2 and 4g / L, the pH of the medium is 5.5. The two mediums were coded as No. 1 and No. 2 respectively.
[0039] 2. Inoculate the domesticated Candida utilis (C...
Embodiment 3
[0043] Fermentation of Candida utilis in a liquid medium with enzymatic hydrolyzate of corn starch as carbon source and waste water with different concentrations of amino acids as nitrogen source.
[0044] 1. Prepare two kinds of liquid fermentation medium for high salinity amino acid wastewater with different concentrations. Liquid fermentation media with high salinity amino acid wastewater concentration of 165g / L and 330g / L were prepared respectively, wherein the high salinity amino acid wastewater was the only nitrogen source (its source and composition were consistent with Example 1), and the liquid fermentation culture The base also adds other chemical substrates required by microorganisms, and its components are: corn starch enzymatic hydrolysis solution (according to the dry weight of corn starch): 55 and 110g / L, KH 2 PO 4 : 7.5 and 15g / L, yeast powder 0 and 10g / L, MgSO 4 : 4.5 and 9g / L, CaCO 3 : 1 and 2g / L, FeSO 4 : 2 and 4g / L, the pH of the medium is 5.5. The two...
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