Method for producing candida for feeds by fermenting high-salt-content amino acid wastewater

A Candida and amino acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable treatment of amino acid wastewater, high cost of concentration treatment process, high investment and operation costs, and achieve nitrogen The effect of high resource utilization efficiency, high application and promotion potential, and strong competitive advantage

Inactive Publication Date: 2014-03-19
HUBEI UNIV OF TECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported amino acid wastewater treatment methods mainly include electrodialysis, ultrafiltration, biochemical methods, etc., but the shortcomings of electrodialysis and ultrafiltration limit the promotion of this method due to high investment and operating costs and inconvenient maintenance; and biochemical methods Because of its low investment and operating costs, mature technology, no secondary pollution, and convenient operation and management, it has received worldwide attention in organic wastewater treatment.
The problem with this method at present is that it is more suitable for medium and low concentration sewage, but it is not suitable for treating high concentration amino acid wastewater
However, the whole treatment method has the disadvantages of high cost, time-consuming and labor-intensive concentration process, disconnection between resource recovery and wastewater treatment, and the fact that organic matter is easy to deteriorate after high-temperature evaporation and concentration.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Acclimatization and cultivation of Candida strains for feed.

[0026] 1. Take the waste liquid from an amino acid factory in Wuhan, after the hydrolysis of hair acid to produce cysteine, and use it as the high-salt amino acid wastewater required below. Its composition table is as follows:

[0027] Table 1 Contents of related elements in amino acid wastewater

[0028] project name

unit

Test results

Method basis

Total Nitrogen (N)

％

8.79

GB / T8572-2010

Sodium (Na)

％

0.52

ICP method

Chlorine (Cl)

g / L

131.26

AgCl nephelometric method

Lead (Pb)

mg / kg

0.91

ICP method

Arsenic (As)

mg / kg

0.076

Atomic Fluorescence

Mercury (Hg)

mg / kg

0.014

Atomic Fluorescence

Chromium (Cr)

mg / kg

1.51

ICP method

Cadmium (Cd)

mg / kg

0.06

ICP method

[0029] Table 2 Amino acid composition and content of amino a...

Embodiment 2

[0037] Fermentation of Candida utilis in liquid medium with glucose as carbon source and waste water with different concentrations of amino acids as nitrogen source.

[0038] 1. Prepare two kinds of liquid fermentation medium for high salinity amino acid wastewater with different concentrations. Liquid fermentation media with high salinity amino acid wastewater concentration of 165g / L and 330g / L were prepared respectively, wherein the high salinity amino acid wastewater was the only nitrogen source (its source and composition were consistent with Example 1), and the liquid fermentation culture The base also adds other chemical substrates required by microorganisms, and its components are: glucose: 45 and 90g / L, KH 2 PO 4 : 7.5 and 15g / L, yeast powder: 5 and 10g / L, MgSO 4 : 4.5 and 9g / L, CaCO 3 : 1 and 2g / L, FeSO 4 : 2 and 4g / L, the pH of the medium is 5.5. The two mediums were coded as No. 1 and No. 2 respectively.

[0039] 2. Inoculate the domesticated Candida utilis (C...

Embodiment 3

[0043] Fermentation of Candida utilis in a liquid medium with enzymatic hydrolyzate of corn starch as carbon source and waste water with different concentrations of amino acids as nitrogen source.

[0044] 1. Prepare two kinds of liquid fermentation medium for high salinity amino acid wastewater with different concentrations. Liquid fermentation media with high salinity amino acid wastewater concentration of 165g / L and 330g / L were prepared respectively, wherein the high salinity amino acid wastewater was the only nitrogen source (its source and composition were consistent with Example 1), and the liquid fermentation culture The base also adds other chemical substrates required by microorganisms, and its components are: corn starch enzymatic hydrolysis solution (according to the dry weight of corn starch): 55 and 110g / L, KH 2 PO 4 : 7.5 and 15g / L, yeast powder 0 and 10g / L, MgSO 4 : 4.5 and 9g / L, CaCO 3 : 1 and 2g / L, FeSO 4 : 2 and 4g / L, the pH of the medium is 5.5. The two...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for producing candida for feeds by fermenting high-salt-content amino acid wastewater, which comprises the following steps: 1. habituated culture of feed strain: carrying out continuous habituated culture to enhance the tolerance of the strain to high-salt-content amino acid wastewater; and 2. fermentation of strain: under appropriate fermenting conditions, carrying out liquid ventilation culture in a fermentation tank for some time to obtain the microzyme for feeds. The microzyme is centrifugated, collected, dried in a 70 DEG C drying oven to constant weight, and weighed. The calculation indicates that every liter of high-salt-content amino acid wastewater can obtain 145g of candida at the maximum. The method can effectively utilize amino acids, microelements and growth factors contained in the wastewater, greatly lowers the production cost of the candida for feeds, greatly reduces the sewage treatment load, achieves the comprehensive utilization effect, and has obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of comprehensive utilization of waste water resources, and more specifically relates to a method for producing candida for feed by fermenting high-salt amino acid waste water. Background technique [0002] According to the World Health Organization, 70% of people in the world do not have access to safe and hygienic drinking water, and 25,000 people die every day from diseases related to sewage or poor sanitation; by 2050, it is estimated that there will be about 700,000 people in the world Millions of people face water shortages. 82% of the people in our country drink water from shallow wells and rivers, 75% of which are polluted by bacteria exceeding the hygienic standard, and the drinking water population polluted by organic matter is about 160 million. The sewage discharged by industry is the main source of water pollution. According to survey statistics, the national annual wastewater discharge is 65.92 billion ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/72
Inventor 胡征杨波刘志刚
Owner HUBEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap