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Heat-resistant freeze-drying protective agent for animal live vaccine, preparation method and application thereof

A heat-resistant freeze-drying and live vaccine technology, applied in freeze-drying delivery, antiviral agents, pharmaceutical formulations, etc., can solve the problems of high production cost, complex heat-resistant protective agent components, and unsatisfactory protective performance, and achieve preservation. Long time, good protective effect, suitable for mass production

Active Publication Date: 2014-03-26
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used heat-resistant protective agent components are too complex; the production cost is high; the protection performance is not ideal

Method used

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  • Heat-resistant freeze-drying protective agent for animal live vaccine, preparation method and application thereof
  • Heat-resistant freeze-drying protective agent for animal live vaccine, preparation method and application thereof
  • Heat-resistant freeze-drying protective agent for animal live vaccine, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of embodiment 1 heat-resistant porcine reproductive and respiratory syndrome virus (CH1R strain) live vaccine

[0024] (1) Formula and preparation method of lyoprotectant

[0025] The formula of lyoprotectant is: lactose 8%, hydrolyzed lactalbumin 1%, polyvinylpyrrolidone 2%, sodium glutamate 0.5%, arginine hydrochloride 0.5%, hydrolyzed silk protein 3%, double distilled water 100mL; Sterilize at 110°C for 15 minutes to obtain a heat-resistant lyoprotectant.

[0026] (2) Shake the heat-resistant lyoprotectant prepared in (1) and the qualified PRRSV-CH1R virus antigen according to the volume ratio of 1:1, and freeze-dry after quantitatively dispensing. At the same time, the sucrose skim milk protectant vaccine was set as the control group.

[0027] (3) Vaccine technical parameters and storage potency determination

[0028] The physical properties, sterility test, mycoplasma test, exogenous virus test, safety test, residual moisture test and vacuum degr...

Embodiment 2

[0035] Embodiment 2 Preparation of heat-resistant highly pathogenic porcine reproductive and respiratory syndrome virus (JXA1-R strain) live vaccine

[0036] (1) Formula and preparation method of lyoprotectant

[0037] The formula of lyoprotectant is: lactose 20%, hydrolyzed lactalbumin 4%, polyvinylpyrrolidone 0.5%, sodium glutamate 2%, arginine hydrochloride 0.5%, hydrolyzed silk protein 1%, double distilled water 100ml; Sterilize at 110°C for 15 minutes to obtain a heat-resistant lyoprotectant.

[0038] (2) Shake the heat-resistant lyoprotectant prepared in (1) and the qualified PRRSV-JXA1-R virus antigen according to the volume ratio of 1:1, and freeze-dry after quantitatively dispensing. At the same time, the sucrose skim milk protectant vaccine was set as the control group.

[0039] (3) Vaccine technical parameters and storage potency determination

[0040] The physical properties, sterility test, mycoplasma test, exogenous virus test, safety test, residual moisture t...

Embodiment 3

[0047] Preparation of embodiment 3 heat-resistant pseudorabies virus (Bartha-K61 strain) live vaccine

[0048] (1) Formula and preparation method of lyoprotectant

[0049] The formula of lyoprotectant is: lactose 16%, hydrolyzed lactalbumin 4%, polyvinylpyrrolidone 0.5%, sodium glutamate 2%, arginine hydrochloride 2%, hydrolyzed silk protein 1%, mannitol 2%, Double-distilled water 100ml; sterilized at 110°C for 15 minutes, it is a heat-resistant freeze-drying protective agent.

[0050] (2) Shake the heat-resistant lyoprotectant prepared in (1) and the qualified PRV-Bartha-K61 virus antigen according to the volume ratio of 1:1, and freeze-dry after quantitatively dispensing. At the same time, the sucrose skim milk protectant vaccine was set as the control group.

[0051] (3) Vaccine technical parameters and storage potency determination

[0052] The physical properties, sterility test, mycoplasma test, exogenous virus test, safety test, residual moisture test and vacuum degree...

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Abstract

The invention provides a heat-resistant freeze-drying protective agent for an animal live vaccine. Each liter of the heat-resistant freeze-drying protective agent comprises the components by weight: 80-200g / L of lactose, 10-40g / L of lactalbumin hydrolysate, 5-20g / L of polyvinylpyrrolidone, 0-20g / L of mannitol, 5-20g / L of sodium glutamate, 5-15g / L of arginine monohydrochloride and 10-30g / L of hydrolyzed silk protein and the agent is prepared by autoclaved sterilization after water is added to dissolve the above components. The freeze-drying protective agent is mixed with a virus solution, and frozen and dried, so as to obtain the freeze-dried vaccine. The heat-resistant freeze-drying protective agent disclosed by the invention is simple in formula, easy to prepare, and applicable to large-scale production, and has a good protection effect on the vaccine. The prepared vaccine is safe and sterile, and stable in quality, and has the characteristics of being heat-resistant and long in preservation time, and the problems that the vaccine needs to be frozen at a low temperature in the transportation process, and is inconvenient to store and the like are solved. By adopting the heat-resistant freeze-drying protective agent provided by the invention, a porcine reproduction and respiratory syndrome vaccine and a pseudorabies live vaccine can be stored for 24 hours at 2-8 DEG C.

Description

technical field [0001] The invention relates to the field of veterinary biological products, in particular to a heat-resistant lyoprotectant for live animal vaccines, a preparation method and application thereof. Background technique [0002] With the development of bioengineering technology, biochemistry, and molecular biology, important progress has been made in the types and types of animal vaccines of veterinary biological products, and various new vaccines have been successfully developed. It can be roughly divided into: inactivated vaccines, attenuated vaccines, monovalent vaccines, multivalent vaccines, mixed vaccines, homologous vaccines, subunit vaccines, and genetically engineered vaccines. [0003] Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is an acute, highly pathogenic infectious disease caused by a variant strain of porcine reproductive and respiratory syndrome virus (PRRSV), which can cause reproductive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/32A61K47/26A61K47/18A61K9/19A61K39/245A61K39/12A61P31/14A61P31/20
Inventor 马良刘萌邵葳周建民潘春刚
Owner CHINA ANIMAL HUSBANDRY IND
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