A kind of composite flora for degrading electroplating wastewater and its preparation method

A technology of complex bacterial flora and electroplating wastewater, applied in the direction of microbial-based methods, biochemical equipment and methods, metallurgical wastewater treatment, etc., can solve the problems of species, small quantity, complex wastewater components, etc., and achieve good adsorption effect

Inactive Publication Date: 2016-05-04
SOUTH CHINA UNIV OF TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organic substances in the environment, and it At a disadvantage in interspecies competition, traditional biological treatment technologies face great challenges

Method used

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  • A kind of composite flora for degrading electroplating wastewater and its preparation method
  • A kind of composite flora for degrading electroplating wastewater and its preparation method
  • A kind of composite flora for degrading electroplating wastewater and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0025] Agrobacterium sp., Bacillus sp., Pseudomonasputida., Pseudomonasstutzeri. were picked respectively (isolated from the sludge at the outlet of the electroplating factory 1 ring of the obtained) four kinds of bacteria, which were transferred to 20mL nutrient solution containing 1.5g / L beef extract, 1.0g / L glucose, 5.5g / L tryptone, 3.0g / L yeast powder, pH6 .5, and the rest is water), each type of bacteria was cultured at 27°C for 1 day, and then the cultured four kinds of bacteria were inoculated at a volume ratio of 1:9 (bacteria and proliferation medium) The suspension was placed in a container containing 500 mL of sterile proliferation medium, cultured at 35° C. for 1 day, centrifuged at 6000 rpm for 10 min, and logarithmic growth phase cells of the above four bacteria were obtained respectively. Adopt different proliferation nutrient medium to cultivate, wherein, the nutrient medium of Agrobacterium and bacillus is beef extract 3.0g / L, tryptone 4.0g / L, the rest is wate...

Embodiment 2

[0033] Pick 2 rings of four kinds of bacteria: Agrobacterium sp., Bacillus sp., Pseudomonasputida., Pseudomonasstutzeri. (from the drainage of the electroplating factory mouth

[0034] Separated from the sludge), transfer them to 30mL nutrient solution (the composition is 2.0g / L beef extract, 1.5g / L glucose, 6.5g / L tryptone, 3.5g / L yeast powder, pH7 .5, the rest is water and essential impurities), each type of bacteria was cultured at 30°C for 2 days, and then the cultured four kinds of bacteria were respectively mixed in a volume ratio of 1:10 (bacteria and Proliferation medium) Inoculate the bacterial suspension into a container containing 500mL of sterile proliferation medium, culture at 35°C for 2 days, centrifuge at 7000rpm for 15min, and obtain the logarithmic growth phase cells of the above four bacteria respectively . Among them, four different bacteria adopt different proliferation medium, wherein the nutrient medium of Agrobacterium and rod-shaped bacteria is beef ...

Embodiment 3

[0043] Pick four rings of bacteria respectively: Agrobacterium sp., Bacillus sp., Pseudomonasputida., Pseudomonasstutzeri. (from the drainage of the electroplating factory Separation from the sludge in the mouth), transfer them to 25mL nutrient solution (the composition is 1.7g / L beef extract, 2.0g / L glucose, 6.0g / L tryptone, 4.0g / L yeast powder, pH 7.0, the rest is water), each type of bacteria was cultured at 27°C for 2 days, and then the cultured four kinds of bacteria were inoculated at a volume ratio of 1:12 (bacteria and proliferation medium) The bacterial suspension was cultured at 30°C for 2 days in a container containing 450 mL of sterile growth medium, centrifuged at 6000 rpm for 15 min, and the logarithmic growth phase cells of the above four bacteria were obtained respectively. Among them, four different bacteria adopt different proliferation medium, wherein the nutrient medium of Agrobacterium and bacillus is beef extract 4.0g / L, tryptone 4.5g / L, and the rest is w...

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Abstract

The invention discloses a complex bacterium for degrading electroplating wastewater and a preparation method of complex bacterium. The method comprises the following steps: respectively picking Agrobacterium sp., Bacillus sp., Pseudomonas putida., Pseudomonas stutzeri. for inoculated culture according to volume percent, respectively activating 15-17% of Agrobacterium sp., 3-17% of Bacillus sp., 23-32% of Pseudomonas putida. and 34-59% of Pseudomonas stutzeri., mixing and feeding into wastewater. The degrading efficiency of microbiologically treating the electroplating wastewater can be effectively improved through synergism among bacterium.

Description

technical field [0001] The invention relates to the field of electroplating wastewater treatment, in particular to a composite bacterial group for degrading electroplating wastewater and a preparation method thereof. Background technique [0002] Due to the non-biodegradability and bioaccumulation of heavy metal ions, as a key industry polluted by heavy metals, electroplating is one of the most serious polluting industries in the world today, and the treatment of heavy metal wastewater has become the focus of attention in the field of environmental protection. Traditionally, the methods for treating heavy metal wastewater are mainly physical and chemical methods, such as adsorption, ion exchange, chemical precipitation, membrane separation, redox and so on. However, these methods all have serious secondary pollution and high processing costs. [0003] The biological treatment process of electroplating wastewater is mainly through the natural adsorption of metals by organism...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/40C12R1/38C02F103/16
Inventor 陈元彩吕源财胡勇有
Owner SOUTH CHINA UNIV OF TECH
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