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Bacillus subtilis ansb0e1 and its application

A technology of ANSB0E1 and Bacillus subtilis, which is applied in the field of microbiology, can solve the problems of less zearalenone and no simultaneous degradation of zearalenone by Bacillus subtilis. 1. Moderate effect

Active Publication Date: 2015-08-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on bacterial degradation of zearalenone
[0004] So far, there is no report about the simultaneous degradation of zearalenone, α-zearalenol and β-zearalenol by Bacillus subtilis and its metabolites

Method used

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  • Bacillus subtilis ansb0e1 and its application
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  • Bacillus subtilis ansb0e1 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The cultivation method of embodiment 1 bacillus subtilis ANSBOE1

[0036] Take 1 mL of Bacillus subtilis ANSB0E1 (CGMCC No.8515) (the concentration of live bacteria is 10 9 CFU / mL), inoculated in 50mL medium for shake flask fermentation, the fermentation temperature was 37°C, the pH value was 7.2, the rotation speed was 200r / min, and the fermentation time was 24h.

[0037] Among them, the shake flask fermentation medium is composed of the following components: tryptone 10g, yeast extract 1.5g, glucose 5g, beef extract 3g, sodium chloride 6g, disodium hydrogen phosphate 2.5g, magnesium sulfate heptahydrate 1g and distilled water 1000mL, pH 7.0.

[0038] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

Embodiment 2

[0039] The cultivation method of embodiment 2 Bacillus subtilis ANSBOE1

[0040]Take 1 mL of Bacillus subtilis ANSB0E1 (CGMCC No.8515) (the concentration of live bacteria is 10 9 CFU / mL), inoculated in 80mL medium for shake flask fermentation, the fermentation temperature was 25°C, the pH value was 7.5, the rotation speed was 225r / min, and the fermentation time was 28h.

[0041] Among them, the shake flask fermentation medium is composed of the following components: tryptone 12g, yeast extract 2g, glucose 4g, beef extract 2g, sodium chloride 5g, disodium hydrogen phosphate 3g, magnesium sulfate heptahydrate 0.5g and distilled water 800mL , pH value is 7.2.

[0042] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

Embodiment 3

[0043] The cultivation method of embodiment 3 Bacillus subtilis ANSBOE1

[0044] Take 1 mL of Bacillus subtilis ANSB0E1 (CGMCC No.8515) (the concentration of live bacteria is 10 9 CFU / mL), inoculated in 100mL medium for shake flask fermentation, the fermentation temperature was 45°C, the pH value was 6.8, the rotational speed was 185r / min, and the fermentation time was 18h.

[0045] Among them, the shake flask fermentation medium is composed of the following components: tryptone 7g, yeast extract 2.5g, glucose 3g, beef extract 4g, sodium chloride 4g, disodium hydrogen phosphate 3.5g, magnesium sulfate heptahydrate 1.25g and Distilled water 1200mL, pH value is 7.4.

[0046] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

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Abstract

The invention provides a bacillus subtilis ANSB0E1 as well as a culture method and applications thereof, wherein the bacillus subtilis ANSB0E1 with the preservation number of CGMCC No.8515 is used for degrading zearalenone, alpha-zearalenol and beta-zearalenol. The invention also provides a method for degrading the zearalenone, alpha-zearalenol and beta-zearalenol by utilizing the fermentation liquor of the strain ANSB0E1. The fermentation liquor reacts with zearalenone for 72 hours, and the degradation rate can achieve more than 88%; the fermentation liquor reacts with alpha-zearalenol for 72 hours, and the degradation rate can achieve more than 83%; and the fermentation liquor reacts with beta-zearalenol for 72 hours, and the degradation rate can achieve more than 80%. The bacillus subtilis is high in degradation activity, strong in specificity, mild in reaction effect, incapable of damaging nutrition ingredients in a feed, applicable to removal of zearalenone, alpha-zearalenol and beta-zearalenol in the feed.

Description

technical field [0001] The invention belongs to the field of microbiology, and in particular relates to a strain of Bacillus subtilis ANSBOE1 and its application. Background technique [0002] Mycotoxin is a secondary metabolite produced during the growth of mold, which is highly toxic, mutagenic and carcinogenic, and seriously threatens the growth and development of animals and human health. At present, the mycotoxins that pose the greatest threat to animal husbandry and humans mainly include aflatoxin (Aflatoxin, AFT), zearalenone (Zearalenone, ZEN), trichothecenes (TRT), ochratoxin ( Ochratoxin A, OTA) and fumonisins (Fumonisins), etc. Zearalenone, also known as F-2 toxin, is a non-steroidal mycotoxin produced by Fusarium fungi, which can bind to estrogen receptors in the body to produce estrogenic effects. α-zearalenol and β-zearalenol are derivatives of zearalenone, which also have the same estrogenic effect as zearalenone, and are more toxic than zearalenone. In hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L1/015A23K1/16C12R1/125A23L5/20
Inventor 计成张建云雷元培马秋刚赵丽红
Owner CHINA AGRI UNIV
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