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Alcohol-free extraction method of polymalic acid

A technology of polymalic acid and its extraction method, which is applied in the field of polymalic acid extraction and polymalic acid industrial production, to achieve the effects of reduced production cost, good quality, and safe and reliable production process

Active Publication Date: 2014-04-09
天津慧智百川生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the existing polymalic acid extraction method, and provide an advanced polymalic acid extraction method: the whole process does not need to add organic solvent alcohol precipitation, and does not need to add organic or inorganic chemical flocculant, mainly It uses physical methods to remove impurities in the fermentation broth. When the content of polymalic acid in the fermentation broth reaches the standard content, vacuum drying is used to obtain polymalic acid with high content and purity, which effectively avoids the potential harm caused by organic solvents. Improve product quality and yield, reduce the high production cost caused by the consumption of strong acid and alkali when using organic solvents or ion exchange resins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] ⑴ Bacteria removal: set the viscosity to 300mm 2 / s fermentation broth is diluted with deionized water twice, and 0.7g of 100-mesh diatomite is added to every 100mL of diluted fermentation broth, and a 500-mesh filter cloth is used to pre-coat diatomite, and a plate-and-frame filter is used for filtration. The operating pressure is 0.22Mpa. Remove bacteria.

[0031] (2) One-time ultrafiltration to remove polysaccharides: Pass the fermented broth after removing bacteria through a membrane module with a molecular weight cut-off of 10,000 daltons, add 0.5 times the volume of deionized water to the fermented broth, circulate ultrafiltration, and operate at a pressure of 0.1MPa, and pour it into the permeate until the conductivity no longer changes.

[0032] (3) Further removal of polysaccharides: add 3% (V / V) pullulanase to the permeate, the operating temperature is 55°C, the pH is 7, and the treatment time is 1 hour to further remove pullulan.

[0033] (4) One-time activ...

Embodiment 2

[0039]⑴ Bacteria removal: set the viscosity to 300mm 2 / s fermentation broth is diluted with deionized water by one time, every 100mL diluted fermentation broth is added with 1g of 300-mesh diatomite, pre-coated with diatomite with 600-mesh filter cloth, and filtered by a plate and frame filter with an operating pressure of 0.14Mpa. bacteria.

[0040] (2) One-time ultrafiltration to remove polysaccharides: pass the fermented broth after removing the bacteria through the membrane module with a molecular weight cut-off of 30,000daltons, add 0.5 times the volume of deionized water to the fermented broth, and circulate the ultrafiltration with an operating pressure of 0.1MPa to the permeate until the conductivity no longer changes.

[0041] (3) Further removal of polysaccharides: Add 2.5% (V / V) pullulanase to the permeate, operate at 55°C, pH 5, and treat for 1 hour to further remove pullulan.

[0042] (4) One-time activated carbon decolorization: heat the fermentation broth tre...

Embodiment 3

[0048] ⑴ Bacteria removal: set the viscosity to 180mm 2 / s fermentation broth was diluted 0.5 times with deionized water, and 1.5g of 200-mesh diatomite was added to every 100mL of diluted fermentation broth, and 800-mesh filter cloth was used to pre-coat diatomite, and a plate-and-frame filter was used for filtration. The operating pressure was 0.24Mpa. Remove bacteria.

[0049] (2) One-time ultrafiltration to remove polysaccharides: pass the fermented broth after removing the bacteria through a membrane module with a molecular weight cut-off of 50,000 daltons, add 0.5 times the volume of deionized water to the fermented broth, and circulate ultrafiltration at an operating pressure of 0.1MPa to the permeate until the conductivity no longer changes.

[0050] (3) Further removal of polysaccharides: Add 1% (V / V) pullulanase to the permeate, the operating temperature is 55°C, the pH is 5, and the treatment time is 1 hour to further remove pullulan.

[0051] (4) One-time activat...

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Abstract

The invention discloses an alcohol-free extraction method of polymalic acid and belongs to the technical field of biology. The alcohol-free extraction method comprises the following major steps of: taking kieselguhr as a filter aid and filtering out bacteria by using a plate and frame filter press; removing polysaccharides by using pullulanase in combination with ultrafiltration; combining activated carbon with plate and frame filtering, decoloring and removing the added activated carbon and denatured protein flocculate; desalting through nanofiltration and concentrating; heating to the range from 60 to 90 DEG C and carrying out rotary evaporation by using a rotary evaporator until the content of the polymalic acid is 20-30g / 100mL, and then spreading on a glass vessel by the thickness ranging from 0.1 to 1.0mm for drying until constant weight, and finally, crushing and packaging to obtain the finished product of the polymalic acid. No organic solvent is added in the whole process; impurities in the fermentation liquid are removed mainly by a physical method, so that potential hazards caused by the organic solvent can be effectively avoided; and a result, the quality and yield of the product are improved, and high production cost due to the consumption of a strong acid and a strong base when the organic solvent or ion exchange resin is used is reduced.

Description

technical field [0001] The invention relates to the industrial production technology of polymalic acid, belongs to the field of biotechnology, in particular to an extraction method of polymalic acid. Background technique [0002] Nowadays, most synthetic polymers are petrochemical products, and their disposal has many adverse effects on the environment because they are not degradable. With the increasing awareness of environmental protection, the proportion of traditional non-degradable polymers in polymer production is gradually decreasing. At present, people are focusing on environmentally friendly biodegradable polymers. The production of these polymers is conducive to saving energy and resources, slowing down the greenhouse effect, reducing environmental pollution, and is conducive to the development of ecological protection methods. [0003] Polymalic acid (polyhydroxysuccinate, Polymalicacid or Polymalate, PMLA for short) is a homopolymer polymer synthesized from mali...

Claims

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Application Information

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IPC IPC(8): C08G63/06
Inventor 乔长晟孟迪宋玉民范栩嘉刘艳丽
Owner 天津慧智百川生物工程有限公司
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