Wheat daily ration enzyme containing alkaline xylanase and preparation method of wheat daily ration enzyme
A technology of acidic xylanase and xylanase, which is applied in the field of wheat dietary enzymes containing alkaline xylanase and its preparation, can solve problems such as unmeasured and complex structures, and achieve high activity and tolerance The effect of high temperature and strong stability
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Embodiment 1
[0054] A wheat dietary enzyme containing alkaline xylanase, consisting of the following raw materials in parts by weight:
[0055] Thermophilic Bacillus culture 50 parts, acid xylanase 25 parts, β-glucanase 25 parts, acid protease 25 parts, cellulase 12 parts, amylase 12 parts, β-mannanase 12 parts , 12 parts of Chinese herbal medicine extract, 12 parts of protective agent, 12 parts of activator.
[0056] The acid xylanase, β-glucanase, acid protease, cellulase, amylase and β-mannanase are all food-grade enzyme preparations;
[0057] The preparation method of described thermophilic bacillus culture comprises the steps:
[0058] (1) Activation of strains
[0059] Inoculate the slant strain of the well-preserved thermophilic Bacillus 701 on the slant medium, culture at 37°C for 24 hours to activate the strain, and activate twice in this way;
[0060] The slant medium consists of: 3g of beef extract, 5g of sodium chloride, 10g of peptone, 2g of glucose, 15g of agar, 5g of Chin...
Embodiment 2
[0096] A wheat dietary enzyme containing alkaline xylanase, consisting of the following raw materials in parts by weight:
[0097] 40 parts of thermophilic Bacillus culture, 20 parts of acid xylanase, 20 parts of β-glucanase, 20 parts of acid protease, 10 parts of cellulase, 10 parts of amylase, 10 parts of β-mannanase , 10 parts of Chinese herbal medicine extract, 10 parts of protective agent, 10 parts of activator.
[0098] The acid xylanase, β-glucanase, acid protease, cellulase, amylase and β-mannanase are all food-grade enzyme preparations;
[0099] The preparation method of described thermophilic bacillus culture comprises the steps:
[0100] (1) Activation of strains
[0101] Inoculate the slant strain of the well-preserved thermophilic Bacillus 701 on the slant medium, culture at 41°C for 30 hours to activate the strain, and activate it 3 times;
[0102] The slant medium consists of: 6g of beef extract, 8g of sodium chloride, 15g of peptone, 5g of glucose, 20g of agar...
Embodiment 3
[0137] A wheat dietary enzyme containing alkaline xylanase, consisting of the following raw materials in parts by weight:
[0138] 60 parts of thermophilic Bacillus culture, 30 parts of acid xylanase, 30 parts of β-glucanase, 30 parts of acid protease, 15 parts of cellulase, 15 parts of amylase, 15 parts of β-mannanase , 15 parts of Chinese herbal medicine extract, 15 parts of protective agent, 15 parts of activator.
[0139] The acid xylanase, β-glucanase, acid protease, cellulase, amylase and β-mannanase are all food-grade enzyme preparations;
[0140] The preparation method of described thermophilic bacillus culture comprises the steps:
[0141] (1) Activation of strains
[0142] Inoculate the slant strain of the well-preserved thermophilic Bacillus 701 on the slant medium, culture at 45°C for 36 hours to activate the strain, and activate it 3 times;
[0143] The slant medium consists of: 10g of beef extract, 12g of sodium chloride, 20g of peptone, 5g of glucose, 20g of ...
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