A production method of feed additive "Acremonium acremonium culture"
A feed additive, Acremonium terrestris technology, which is applied in the production field of feed additive "Acerificus terrestris culture", can solve the problem that the content of active substances in the final product is not up to standard, the bacteria growth period is long and easy to contaminate, and the growth and heat dissipation are not good. Timely and other problems to achieve the effect of enhancing immune regulation ability, improving feed conversion rate, and eliminating the three wastes problem
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Embodiment 1
[0027] The production method of "Acremonium acremonium culture" of the present invention comprises the following production steps:
[0028] A. First-class seed tank production
[0029] Fill about 35L of liquid culture medium into a 50L air-lift fermenter, and sterilize at 121°C to 126°C for 30 minutes; after sterilization, inoculate when cooled to 23°C, the culture temperature is 23°C, and the ventilation rate is 1:1V / V min, holding pressure 0.05 ~ 0.1MPa, cultured with ventilation for 24 hours, after reaching the logarithmic growth phase;
[0030] The mass ratio of raw materials for the first-level seed tank liquid medium is: soybean powder 3%, white sugar 3%, corn flour 2%, yeast powder 1%, yeast extract powder 1%, silkworm chrysalis powder 0.5%, M g SO 4 0.05%, KH 2 PO 4 0.05%, defoamer 0.05%, the rest of water, and use saturated NaOH (sodium hydroxide) to adjust the pH value to 6.5.
[0031] B. Secondary seed tank production
[0032] Put 350L liquid culture medium i...
Embodiment 2
[0037] The production method of "Acremonium acremonium culture" of the present invention comprises the following production steps:
[0038] A. First-class seed tank production
[0039] Fill about 35L of liquid culture medium into a 50L air-lift fermenter, and sterilize at 121°C to 126°C for 30 minutes; after sterilization, inoculate when cooled to 23°C, the culture temperature is 23°C, and the ventilation rate is 1:1V / V min, holding pressure 0.05 ~ 0.1MPa, cultured with ventilation for 24 hours, after reaching the logarithmic growth phase;
[0040] The mass ratio of raw materials for the first-level seed tank liquid medium is: soybean powder 5%, white sugar 5%, corn flour 3%, yeast powder 2%, yeast extract powder 2%, silkworm chrysalis powder 0.8%, M g SO 4 0.06%, KH 2 PO 4 0.06%, defoamer 0.06%, the rest of water, and use saturated NaOH (sodium hydroxide) to adjust the pH value to 6.5.
[0041] B. Secondary seed tank production
[0042] Put 350L liquid culture medium i...
Embodiment 3
[0047] The production method of "Acremonium acremonium culture" of the present invention comprises the following production steps:
[0048] A. First-class seed tank production
[0049] Fill about 35L of liquid culture medium into a 50L air-lift fermenter, and sterilize at 121°C to 126°C for 30 minutes; after sterilization, inoculate when cooled to 23°C, the culture temperature is 23°C, and the ventilation rate is 1:1V / V min, holding pressure 0.05 ~ 0.1MPa, cultured with ventilation for 24 hours, after reaching the logarithmic growth phase;
[0050] The mass ratio of raw materials for the first-level seed tank liquid medium is: soybean powder 2%, white sugar 2%, corn flour 2%, yeast powder 1%, yeast extract powder 1%, silkworm chrysalis powder 0.4%, M g SO 4 0.04%, KH 2 PO 4 0.04%, defoamer 0.04%, the rest of water, and adjust the pH value to 6.5 with saturated NaOH (sodium hydroxide).
[0051] B. Secondary seed tank production
[0052] Put 350L liquid culture medium in ...
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