A production method of feed additive "Acremonium acremonium culture"

A feed additive, Acremonium terrestris technology, which is applied in the production field of feed additive "Acerificus terrestris culture", can solve the problem that the content of active substances in the final product is not up to standard, the bacteria growth period is long and easy to contaminate, and the growth and heat dissipation are not good. Timely and other problems to achieve the effect of enhancing immune regulation ability, improving feed conversion rate, and eliminating the three wastes problem

Active Publication Date: 2016-05-11
合肥河川生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long-term pilot test found that there are problems in the constant temperature solid fermentation of "20℃~30℃ constant temperature solid fermentation": the germination period is long and easy to pollute, the logarithmic growth is too fast and the heat dissipation is not timely and easy to burn the plate
That is: if cultured at a low temperature of 20°C at a constant temperature, the growth of bacteria will be slow, the growth period will be prolonged and easy to be polluted, and the number of bacteria points will cause the growth of the later strains to be weak; if the culture at a high temperature of 30°C at a constant temperature will lead to slow growth and heat dissipation in the logarithmic phase, the temperature will increase. If it is too high, it will cause contamination of the burner and failure of fermentation; ④ solid fermentation inoculum amount 2%~20%
The long-term pilot test found that low inoculum amount can easily lead to problems such as slow germination, poor growth in the later stage, and substandard content of active substances in the final product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The production method of "Acremonium acremonium culture" of the present invention comprises the following production steps:

[0028] A. First-class seed tank production

[0029] Fill about 35L of liquid culture medium into a 50L air-lift fermenter, and sterilize at 121°C to 126°C for 30 minutes; after sterilization, inoculate when cooled to 23°C, the culture temperature is 23°C, and the ventilation rate is 1:1V / V min, holding pressure 0.05 ~ 0.1MPa, cultured with ventilation for 24 hours, after reaching the logarithmic growth phase;

[0030] The mass ratio of raw materials for the first-level seed tank liquid medium is: soybean powder 3%, white sugar 3%, corn flour 2%, yeast powder 1%, yeast extract powder 1%, silkworm chrysalis powder 0.5%, M g SO 4 0.05%, KH 2 PO 4 0.05%, defoamer 0.05%, the rest of water, and use saturated NaOH (sodium hydroxide) to adjust the pH value to 6.5.

[0031] B. Secondary seed tank production

[0032] Put 350L liquid culture medium i...

Embodiment 2

[0037] The production method of "Acremonium acremonium culture" of the present invention comprises the following production steps:

[0038] A. First-class seed tank production

[0039] Fill about 35L of liquid culture medium into a 50L air-lift fermenter, and sterilize at 121°C to 126°C for 30 minutes; after sterilization, inoculate when cooled to 23°C, the culture temperature is 23°C, and the ventilation rate is 1:1V / V min, holding pressure 0.05 ~ 0.1MPa, cultured with ventilation for 24 hours, after reaching the logarithmic growth phase;

[0040] The mass ratio of raw materials for the first-level seed tank liquid medium is: soybean powder 5%, white sugar 5%, corn flour 3%, yeast powder 2%, yeast extract powder 2%, silkworm chrysalis powder 0.8%, M g SO 4 0.06%, KH 2 PO 4 0.06%, defoamer 0.06%, the rest of water, and use saturated NaOH (sodium hydroxide) to adjust the pH value to 6.5.

[0041] B. Secondary seed tank production

[0042] Put 350L liquid culture medium i...

Embodiment 3

[0047] The production method of "Acremonium acremonium culture" of the present invention comprises the following production steps:

[0048] A. First-class seed tank production

[0049] Fill about 35L of liquid culture medium into a 50L air-lift fermenter, and sterilize at 121°C to 126°C for 30 minutes; after sterilization, inoculate when cooled to 23°C, the culture temperature is 23°C, and the ventilation rate is 1:1V / V min, holding pressure 0.05 ~ 0.1MPa, cultured with ventilation for 24 hours, after reaching the logarithmic growth phase;

[0050] The mass ratio of raw materials for the first-level seed tank liquid medium is: soybean powder 2%, white sugar 2%, corn flour 2%, yeast powder 1%, yeast extract powder 1%, silkworm chrysalis powder 0.4%, M g SO 4 0.04%, KH 2 PO 4 0.04%, defoamer 0.04%, the rest of water, and adjust the pH value to 6.5 with saturated NaOH (sodium hydroxide).

[0051] B. Secondary seed tank production

[0052] Put 350L liquid culture medium in ...

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PUM

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Abstract

The invention discloses a production method of a feed additive "acremonium acremonium culture". This production method improves the production process of Acremonium acremonium culture through the research on the growth characteristics of Acremonium acremonium, and shortens the production cycle of Acremonium acremonium culture from the original 11 to 15 days to 5 days after improvement. , greatly reducing the production cost of Acremonium acremonium culture, which is of great significance to the market promotion of this high-end feed additive; at the same time, it greatly reduces the energy consumption of water, electricity and electricity in the production process. The production process of "Acremonium acremonium culture" in the present invention mainly includes "liquid strain fermentation" and "solid fermentation". The results of animal experiments show that the feed additive can significantly improve animal immunity, lower feed-to-meat ratio, and improve meat quality. The method for producing the "acremonium acremonium culture" feed additive has the advantages of low cost, short production cycle, controllable quality, good application effect and the like.

Description

technical field [0001] The invention belongs to the technical field of feed additive processing, and in particular relates to a production method of a feed additive "Acremonium acremonium culture", more specifically using acremonium acremonium ( Acremonium terricola , preservation number CGMCC0346) and a preparation method of acremonium acremonium culture obtained by liquid strain fermentation and solid fermentation. Background technique [0002] Most of the feed additives in the "Catalogue of Feed Additives" issued by the Ministry of Agriculture of my country are traditional antibiotics and chemically synthesized drugs. The transfer of antibiotics has a huge impact on human health, and even threatens the safety of human life; and the human body often ingests low doses of antibiotic residues from meat, which will gradually accumulate in the body and cause lesions in various organs, leading to allergic reactions , immunosuppressive, teratogenic, carcinogenic, mutagenic and oth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K20/195C12N1/14C12R1/645
Inventor 史晓宇谢韬樊美珍黄勃
Owner 合肥河川生物医药科技有限公司
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