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Composite medium for producing functional red yeast rice and its preparation method and fermentation method

A technology of functional red yeast rice and culture medium, which is applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc. Problems such as agglomeration of bases, to achieve the effect of being conducive to rapid growth, improving fermentation effect and product quality, and short fermentation period

Active Publication Date: 2015-11-18
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Functional red yeast rice is traditionally fermented and cultivated using rice as a substrate, and oats, barley, and yam are also used as substrates for cultivation. However, the nutritional content of a single granular substrate is not completely suitable for the growth and development of red yeast rice. The metabolism of Monacolin K, and the powdery material is likely to cause the solid medium to agglomerate during the fermentation process, which is not conducive to heat dissipation, ventilation, and mass transfer and heat transfer, resulting in the existence of some raw materials after fermentation. Lin K's metabolism is slow and the content of the product is low
According to current research reports, the maximum content does not exceed 25mg / g, which restricts the development of functional red yeast rice industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: The raw material ratio, preparation method and the fermentation method of the functional red yeast rice using this compound medium for the production of functional red yeast rice are as follows.

[0024] Weigh indica rice, oats, soybeans and grind them, pass through a 40-mesh sieve, mix 2000g powder according to the ratio of indica rice flour 24wt%, oat flour 42wt%, soybean flour 34wt%; Peptone 5g / L, compound amino acid 1g / L, magnesium sulfate 2g / L, sodium nitrate 1g / L (that is, the number of raw materials contained in each liter of nutrient solution) and water to prepare 700mL nutrient solution, use acidity regulator glacial acetic acid Adjust the pH to 3.5, mix the powder and nutrient solution at a weight ratio of 1:0.35, mix the nutrient solution and powder evenly, control the granulation temperature to 55°C, the particle size to 3mm, and the particle length to 2mm, and granulate through a ring die The equipment produces cylindrical granules with a hard...

Embodiment 2

[0025] Embodiment 2: The raw material ratio, preparation method and the fermentation method of the functional red yeast rice using this compound medium for the production of functional red yeast rice are as follows.

[0026] Weigh indica rice, oats, and soybeans to crush them, pass through a 40-mesh sieve, mix 4000g of powder according to the ratio of indica rice flour 38wt%, oat flour 55wt%, and soybean flour 7wt%; The content of peptone 12g / L, compound amino acid 5g / L, magnesium sulfate 5g / L, sodium nitrate 5g / L is mixed with 2200mL nutrient solution with water, the pH is adjusted to 4.5 with acidity regulator malic acid, and the powder and nutrient solution are mixed according to the weight ratio The ratio of 1:0.55 was mixed evenly with the nutrient solution and the powder, the granulation temperature was controlled at 90°C, the particle size was 5mm, and the particle length was 8mm, and the granules with a hardness of 6.5kg were made through the ring die granulation equipm...

Embodiment 3

[0027] Embodiment 3: The raw material ratio, preparation method and the fermentation method of the functional red yeast rice using this compound medium for the production of functional red yeast rice are as follows.

[0028] Weigh indica rice, oats, and soybeans to crush them, pass through a 70-mesh sieve, mix 2000g powder according to the ratio of indica rice flour 35wt%, oat flour 50wt%, soybean flour 15wt%; Peptone 10g / L, compound amino acid 3g / L, magnesium sulfate 3g / L, sodium nitrate 3g / L content configuration 1200mL nutrient solution, adjust pH to 4.0 with acidity regulator citric acid, powder and nutrient solution according to weight ratio Mix the nutrient solution and powder evenly at a ratio of 1:0.6, control the granulation temperature to 75°C, the particle size to 4mm, and the particle length to 4mm, and make granules with a hardness of 4kg through the ring die granulation equipment; culture the granules Put the base into 1000mL glass triangular flasks, 200g per bot...

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Abstract

The invention discloses a complex medium for producing functional red yeast rice and a preparation method and fermentation method thereof. The complex medium is prepared by granulating powder and nutrient solution, wherein the powder comprises the following raw materials in percentage by weight: 24-38% of long-shaped rice powder, 42-55% of oat powder and 7-34% of soybean meal; the nutrient solution is prepared from water and the following raw materials: 38-76g / L sucrose, 5-12g / L yeast powder, 5-12g / L ox bone peptone, 1-5g / L compound amino acid, 2-5g / L magnesium sulfate, 1-5g / L sodium nitrate and an acidity regulator for regulating the pH value of the nutrient solution to 3.5-4.5; the weight ratio of the powder to the nutrient solution is 1:(0.2-0.6). The complex medium with nutritional ingredients and material characteristics better conforming to monascus fermentation is prepared by optimizing the ratio of raw materials; the looseness, air permeability and mass and heat transfer effect of the medium are improved through raw material granulation, and the complex medium adapts to the growth of monascus strains and the metabolism of monacolin K, thus the fermentation effect and product quality of the functional red yeast rice are effectively improved.

Description

technical field [0001] The invention belongs to the field of microbial solid-state fermentation, in particular to a compound medium for producing functional red yeast rice and a preparation method and fermentation method thereof. Background technique [0002] Red yeast rice, known as Danqu in ancient times, has been used in my country for more than one thousand years. It is a fermented product with the same source of medicine and food. With the discovery of Monacolin K, a metabolite of Monascus, more and more people pay attention to the fermented products of Monascus. [0003] Functional red yeast rice has the effect of lowering blood lipids. It is produced by inoculating Monascus mold on grain medium through solid-state fermentation, and the monacrine K content is greater than 4mg / g. Monascus fermented product. In addition to Mona Jungle K, functional red yeast rice also contains a variety of biologically active ingredients such as ergosterol, γ-aminobutyric acid, and natu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 武亚明王轩李静静张晓武
Owner CHENGUANG BIOTECH GRP CO LTD
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