Primer combination for guiding application of beta-receptor blocker, multi-gene detection kit and using method thereof
A primer composition and receptor blocking technology, applied in biochemical equipment and methods, microbial measurement/testing, recombinant DNA technology, etc., can solve problems such as high technical cost, affecting sensitivity, and difficult conditions to control, and achieve detection High sensitivity, lower false positive rate, and cost-saving detection effects
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[0040] Example 1
[0041] The present invention is a multiple gene detection kit for guiding β-receptor blocking medicinal drugs. When in use, a patient’s blood or buccal swab sample is collected to extract nucleic acid, the patient’s nucleic acid is used as a template for PCR reaction, and the sample is finally separated by capillary electrophoresis. Specific steps are as follows:
[0042] 1. Production of multiple gene detection kits guided by β-receptor blocking medicinal drugs, the components included in the kit:
[0043] 1) PCR Primer Mix
[0044] 2) 25mM magnesium chloride (MgCl 2 )
[0045] 3) Taq DNA Polymerase
[0046] 4) X solution (Solution X)
[0047] 5) PCR Buffer
[0048] 6) Positive Control
[0049] 7) Ultra-pure water (ddH 2 O)
[0050] The above-mentioned PCR primers include the forward and reverse amplification primers of different genotypes at the 3 SNP sites of the following 3 genes related to β-receptor blocking drugs and the forward and reverse amplification primers o...
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[0075] Example 2
[0076] Detection kit specific analysis: single-plex PCR amplification is detected as a single peak of target fragment size by capillary electrophoresis.
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