A method of comprehensively utilizing ginkgo exocarp
A technology of ginkgo testa and testa, which is applied in the direction of botany equipment and methods, applications, special forms of dry distillation, etc., can solve the problems of ginkgo testa polluting the environment, and achieve the effect of solving environmental pollution
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Embodiment 1
[0031] (1) Put the peeled testa of mature ginkgo fruit in a vacuum drying oven at 40°C for 24 hours; take 1kg of the dried testa and crush it through a 40-mesh sieve;
[0032] (2) Heat the crushed testa with ethanol at a material-to-liquid ratio of 1g:4ml to 50°C and reflux for 2 hours, then filter, repeat ethanol reflux and filter 3 times, and combine the filtrates;
[0033] (3) Evaporate the filtrate in vacuum at 20°C to obtain 89g of extract, and then add 2 times the mass of petroleum ether to dissolve and extract; evaporate the petroleum ether extract again in vacuum to obtain 56g of extract, dissolve and extract with 2 times the mass of methanol ;like image 3 Shown is the high performance liquid chromatogram of the methanol extract.
[0034] The HPLC conditions are as follows: HPLC: Shimadzu LC-20AT; column: GL Sciences Inc.Inertsil ODS-SP (4.6mm×150mm, 5μm); detector: SPD-20A UV / VIS ultraviolet-visible light detection detector (detection wavelength: 190-700nm); detect...
Embodiment 2
[0039] (1) Put the peeled testa of mature ginkgo fruit in a vacuum drying oven at 60°C for 36 hours; take 1 kg of the dried testa and crush it through a 60-mesh sieve;
[0040] (2) Heat the crushed testa with ethanol at a material-to-liquid ratio of 1g:5ml to 80°C for reflux for 3 hours, then filter, repeat ethanol reflux and filter 3 times, and combine the filtrates;
[0041] (3) Evaporate the filtrate in vacuum at 40°C to obtain 96g of the extract extract, then add 3 times the mass of petroleum ether to dissolve and extract; evaporate the petroleum ether extract again in vacuum to obtain 62g of extract, dissolve and extract with 3 times the mass of methanol ;
[0042] (4) Prepare 100ml of methanol extract, emulsifier (60wt% calcium dodecylbenzenesulfonate aqueous solution), and dimethyl sulfoxide at a volume ratio of 4:2:2, dilute 1000 times with water, and spray 102m bamboo willow forest , 82.1% mortality rate of Leaf beetle;
[0043](5) Vacuum-dry the filtered testa resi...
Embodiment 3
[0045] (1) Put the peeled testa of mature ginkgo fruit in a vacuum drying oven at 70°C for 48 hours; take 1kg of the dried testa and crush it through an 80-mesh sieve;
[0046] (2) Heat the crushed testa with ethanol at a material-to-liquid ratio of 1g:6ml to reflux at 60°C for 4 hours, then filter, repeat ethanol reflux and filter twice, and combine the filtrates;
[0047] (3) Evaporate the filtrate in vacuum at 40°C to obtain 87g of the extract, and then add 4 times the mass of petroleum ether to dissolve and extract; evaporate the petroleum ether extract again in vacuum to obtain 58g of the extract, dissolve and extract with 3 times the mass of methanol ;
[0048] (4) Methanol extract, emulsifier (30wt% aqueous solution of alkylphenol polyoxyethylene ether, 60wt% aqueous solution of calcium dodecylbenzenesulfonate v:v=5:5), N,N-dimethyl formazan Amide was prepared in 100ml at a volume ratio of 3:3:4, diluted 1000 times with water, sprayed on 102m bamboo willow forest, and ...
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