Application of osmanthus essential oil in anti-caries
A technology of osmanthus essential oil and caries, applied in the application field of osmanthus essential oil in anti-caries, can solve the problems of insignificant anti-caries effect, easy volatility, poor safety, etc., and achieve the effect of obvious osmanthus fragrance, no toxic side effects, and simplified formula
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Embodiment 1
[0018] Accurately weigh 10 kg of washed and dried osmanthus samples, add 30 L of food-grade petroleum ether, extract on a shaking table for 1 hour, and extract twice. Collect the filtrate, distill under reduced pressure to recover petroleum ether, add 100 times of absolute ethanol to the remaining filtrate, reflux at 50°C for 1 hour, collect the liquid at -20°C overnight, freeze and suction filter, evaporate the solvent, and obtain a bright yellow liquid, which is Osmanthus essential oil.
[0019] After detection and analysis, the quality of the obtained osmanthus essential oil is: clear brown-yellow flowing liquid, with the aroma of natural osmanthus; ) was not detected, the arsenic content (As) was not detected, and the characteristic components were 4.0% dihydro-β-ionone and 1.0% ionone.
Embodiment 2
[0021] Accurately weigh 10 kg of washed and dried osmanthus samples, add to 30 L of water, condense and reflux for extraction for 6 hours, and extract twice. Collect the filtrate, extract it with food-grade petroleum ether, and dry it overnight with anhydrous sodium sulfate to obtain a bright yellow liquid, which is osmanthus essential oil.
[0022] After detection and analysis, the quality of the obtained osmanthus essential oil is: clear brown yellow flowing liquid, with the aroma of natural osmanthus; relative density (25 / 25°C) 0.900, refractive index (20°C) 1.440, ester value 25, heavy metal (calculated as Pb) ) was not detected, the arsenic content (As) was not detected, and the characteristic components were 2.5% dihydro-β-ionone and 0.5% ionone.
Embodiment 3
[0024] Accurately weigh 10 kg of washed and dried osmanthus samples, add 30 ml of an aqueous solution containing β-glucosidase, control the pH value at 5.0, and perform enzymatic hydrolysis at 50°C for two hours. After washing with water, dry, add 400 times absolute ethanol, condense and reflux at 55°C for 1 hour, collect the liquid at -20°C overnight, freeze and suction filter, evaporate the solvent, and obtain a bright yellow liquid, which is osmanthus essential oil.
[0025] After detection and analysis, the quality of the obtained osmanthus essential oil is: clear brown yellow flowing liquid, with the aroma of natural osmanthus; ) was not detected, the arsenic content (As) was not detected, and the characteristic components were 0.5% dihydro-β-ionone and 0.06% ionone.
[0026] Therefore, the quality requirements of the osmanthus essential oil used in this example are: clear brownish-yellow flowing liquid with natural osmanthus fragrance; relative density (25 / 25°C) 0.900-0....
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