A kind of preparation method of yogurt

A technology for yogurt and milk volume, applied in milk preparations, dairy products, applications, etc., can solve the problems of insufficient nutritional value and health care function of yogurt, unremarkable typical flavor of fermented milk, long yogurt solidification time, etc., and achieve the best taste Small changes in the amount of whey precipitation, shorten the coagulation time, and reduce the effect of whey separation

Active Publication Date: 2016-04-27
广州华农大食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Generally, when the production raw materials and equipment processes of yogurt are fixed, the quality of the starter becomes the dominant factor in determining the quality of the yogurt, and the quality of the bacteria mainly depends on the stability of the starter in the yogurt. During the preservation process, unstable phenomena are prone to occur, while during the fermentation process, the bacteria grow slowly, produce less acid, and the yogurt coagulates for a long time. The finished yogurt produced has poor sensory quality, severe whey separation, sour taste, Insufficient aroma and acid production, the typical flavor of fermented milk is not outstanding, and the shelf life is short. At the same time, the number of beneficial bacteria contained in yogurt is small and their vitality is low, which cannot achieve the nutritional value and health care function of yogurt itself

Method used

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  • A kind of preparation method of yogurt

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The specific breeding process of described Lactobacillus plantarum is as follows:

[0036] 1. Diethyl Sulfate (DES) Mutagenesis Breeding

[0037] (1) Take a ring of Lactobacillus plantarum on the slant of the test tube on the ultra-clean bench, put it into a 250 mL conical flask containing 50 mL of liquid MRS xylan medium, and cultivate it at 200 rpm and 40 °C for about 12 hours, so that the bacteria are in the opposite direction. number of pre-growth stages.

[0038] (2) Take 5 mL of bacterial liquid, centrifuge at 5000 rpm for 10 min to collect bacterial cells, and wash twice with normal saline.

[0039] (3) Dilute to 10 with pH 7.0 phosphate buffer 7 cells / mL bacterial suspension.

[0040] (4) Take 32 mL of potassium phosphate buffer at pH 7.0, 8 mL of bacterial suspension, and 0.4 mL of DES, and mix them thoroughly in a 150 mL Erlenmeyer flask that was placed in the rotor beforehand, so that the final concentration of DES is 1% (v / v).

[0041] (5) React at 150 r...

Embodiment 2

[0057] A preparation method of yogurt, comprising the steps:

[0058] Raw material processing: acceptance, sterilization, storage, standardization, batching, degassing and homogenization of raw milk;

[0059] Secondary sterilization: After homogenization, the material is sterilized through a heat exchanger, and the conditions are 95°C, 300s;

[0060] Cooling and inoculation: The sterilized material is cooled to 44°C, and the mixed working fermentation inoculum is added in an amount of 3% of the raw milk.

[0061] The mixed working starter is composed of Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis;

[0062] The mass ratio of the Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis is 1:0.2:0.2;

[0063] Fermentation: After the inoculation work is completed, the stirring is started, and the stirring is stopped after 15 minutes. The temperature of the fermenter is kept at 43 ° C, and the fermentation is 3 hours. When ...

Embodiment 3

[0066] A preparation method of yogurt, comprising the steps:

[0067] Raw material processing: acceptance, sterilization, storage, standardization, batching, degassing and homogenization of raw milk;

[0068] Secondary sterilization: After homogenization, the material is sterilized through a heat exchanger, and the conditions are 95°C, 300s;

[0069] Cooling and inoculation: The sterilized material is cooled to 44°C, and the mixed working fermentation bacterial agent is added, and the amount added is 4% of the raw milk.

[0070] The mixed working starter is composed of Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis;

[0071] The mass ratio of the Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis is 1:0.4:0.3;

[0072] Fermentation: After the inoculation work is completed, the stirring is started, and the stirring is stopped after 15 minutes. The temperature of the fermenter is kept at 45 ° C, and the fermentation i...

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PUM

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Abstract

The invention discloses a preparation method of yoghourt, and belongs to the field of processing and manufacturing of dairy products. The preparation method of the yoghourt comprises the following steps: material treatment, secondary sterilization, cooling, inoculating, fermenting, cooling, crushing curd, bulking, cooling and after-ripening, wherein the added mixing work leavening agent is prepared from lactobacillus plantarum, lactobacillus bulgaricus and bifidobacterium animalis in the cooling and inoculating steps. The yoghourt is produced by mixed strains of the lactobacillus plantarum, the lactobacillus bulgaricus and the bifidobacterium animalis, the mixed strains can greatly grow and breed, and produce acids at the early stage of yogurt fermentation. Thus, the coagulation time of the yoghourt is shortened, the coagulation state of the yoghourt is improved, whey separation is reduced, the yoghourt is appropriate and refresh in tart flavor, has typical outstanding fermented milk flavor, not only is the fermentation speed of sour milk quickened, but also the production cycle is also shortened by strong acid yield of the lactobacillus plantarum, and the costs in the aspects such as manpower, energy consumption, equipment and the like are saved.

Description

technical field [0001] The invention belongs to the field of dairy product processing and manufacturing, and in particular relates to a preparation method of yogurt. Background technique [0002] Yogurt is a fermented milk product prepared by adding fresh milk or reconstituted milk as raw material, adding lactic acid bacteria starter after pasteurization, and fermenting at about 40°C. Yogurt is divided into pure yogurt, flavored yogurt, and fruit yogurt. Pure yogurt refers to a product made of milk or reconstituted milk after inoculation and fermentation; flavored yogurt is made from 65-80% milk or reconstituted milk, inoculated and fermented or added with other ingredients such as flavoring agents. Fruit yogurt refers to products made from 50-80% or more of milk or reconstituted milk as raw materials, adding flavorings, fruits and vegetables, grains and other raw materials before or after inoculation and fermentation. All kinds of yogurt products are very popular among co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C9/123
Inventor 郭建峰史楠马文兵马忠平
Owner 广州华农大食品科技有限公司
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