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Neutral protease-containing coarse grain daily ration enzyme and preparation method thereof

A technology of neutral protease and acid protease, which is applied in the field of miscellaneous grain dietary enzymes and its preparation, can solve the problems of complex structure and inability to measure, and achieve the effect of high temperature tolerance and wide range of applicable pH value

Active Publication Date: 2015-03-25
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the structure of the market is relatively complex, the market is diversified, and it is even impossible to measure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A miscellaneous grain dietary enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0058] 25 parts of Bacillus subtilis culture, 40 parts of acid xylanase, 25 parts of β-glucanase, 25 parts of acid protease, 12 parts of pectinase, 12 parts of cellulase, 12 parts of amylase, Chinese herbal medicine extract 12 parts, 12 parts of protective agent, 12 parts of activator.

[0059] Described acid xylanase, β-glucanase, acid protease, pectinase, cellulase, amylase are all food-grade enzyme preparations;

[0060] The preparation method of described bacillus subtilis culture comprises the steps:

[0061] (1) Inoculate the well-preserved slant strain of Bacillus subtilis 1398-2-12 on the slant medium, culture at 30°C for 24 hours to activate the strain, and activate it twice;

[0062] The slant medium consists of: beef extract 3g, sodium chloride 5g, peptone 10g, glucose 2g, (NH) 2 SO 4 3g, K 2 HPO 4 6g, CaCl 2 1g, 15g a...

Embodiment 2

[0096] A miscellaneous grain dietary enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0097] 20 parts of Bacillus subtilis culture, 30 parts of acid xylanase, 20 parts of β-glucanase, 20 parts of acid protease, 10 parts of pectinase, 10 parts of cellulase, 10 parts of amylase, Chinese herbal medicine extract 10 parts, 10 parts of protective agent, 10 parts of activator.

[0098]Described acid xylanase, β-glucanase, acid protease, pectinase, cellulase, amylase are all food-grade enzyme preparations;

[0099] The preparation method of described bacillus subtilis culture comprises the steps:

[0100] (1) Activation of strains

[0101] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 33°C for 30 hours to activate the strain, and activated twice in this way;

[0102] The slant medium consists of: beef extract 6g, sodium chloride 8g, peptone 15g, glucose 4g, (NH)...

Embodiment 3

[0135] A miscellaneous grain dietary enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0136] 30 parts of Bacillus subtilis culture, 50 parts of acid xylanase, 30 parts of β-glucanase, 30 parts of acid protease, 15 parts of pectinase, 15 parts of cellulase, 15 parts of amylase, Chinese herbal medicine extract 15 parts, 15 parts of protective agent, 15 parts of activator.

[0137] Described acid xylanase, β-glucanase, acid protease, pectinase, cellulase, amylase are all food-grade enzyme preparations;

[0138] The preparation method of described bacillus subtilis culture comprises the steps:

[0139] (1) Activation of strains

[0140] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 36°C for 36 hours to activate the strain, and activated 3 times in this way;

[0141] The slant medium consists of: beef extract 10g, sodium chloride 12g, peptone 20g, glucose 5g,...

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Abstract

The invention discloses a neutral protease-containing coarse grain daily ration enzyme and a preparation method thereof, belonging to the technical field of preparation of enzyme preparation. The coarse grain daily ration enzyme is prepared by scientifically compounding bacillus subtilis culture, acidic xylanase, beta-glucanase, acidic protease, pectinase, cellulose, amylase, Chinese herb extracts, a protective agent and an activating agent, wherein the bacillus subtilis culture has the functions of improving animal organism immunity, promoting the development of immune organs and the maturity of animal intestinal tract structure and function and improving the animal daily gain and the feed conversion rate. After the neutral protease-containing coarse grain daily ration enzyme is adopted, safe digestive enzyme is provided for livestock and poultry, the digestion burden is effectively lightened, the utilization rate and the growth rate of the raw materials are improved, and the environment is protected; furthermore, the right amount of activating agent gives full play to the effect of the enzyme preparation under the same condition, so that the add amount of the enzyme preparation is reduced; the Chinese herb extracts are scientifically compounded, so that the shelf life of the compound enzyme preparation can be prolonged, and the immunity of the raised livestock and poultry can be improved.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparation, and in particular relates to a miscellaneous grain dietary enzyme containing neutral protease and a preparation method thereof. Background technique [0002] There are four main reasons for adding enzyme preparations to feed: 1. Degradation of anti-nutritional factors present in animal feed. These substances cannot be degraded by animal endogenous enzymes, thereby interfering with the normal metabolism of animals, resulting in indigestion of animals and decreased production performance. 2. Improve the utilization rate of starch, protein and minerals. These substances are either surrounded by fiber-rich cell walls, or are present in certain structures that cannot be digested by animals (for example, in plant feed materials, a large amount of phosphorus is present in the form of phytate phosphorus.). 3. Degrade some specific chemical bonds in raw materials. These chemical bonds cannot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/165A23K1/14A23K1/00C12N1/20C12N9/42C12N9/26C12N9/24C12N9/18C12N9/50C12N9/56C12N9/88
Inventor 李洪兵张锦杰李海清朱永明刘文明
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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