Method for improving output of alpha-arbutin

A technology of arbutin and yield, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of low conversion rate of α-arbutin, large equipment investment and operating costs, and poor The problem of low tolerance of diphenols is to achieve the effect of simplifying the separation and purification process and its equipment investment, improving tolerance and low production cost

Inactive Publication Date: 2014-05-07
BEIJING UNIV OF CHEM TECH
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The tolerance of the catalyst (bacteria or enzyme) to hydroquinone is not high, resulting in a low yield of α-arbutin;
[0007] 2. The conversion rate of α-arbutin is not high, resulting in a low utilization rate of raw materials;
[0008] 3. The subsequent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040] Example 1

[0041] (1) Preparation of immobilized hydroquinone:

[0042] First prepare a hydroquinone aqueous solution with a concentration of 10% by mass; then add 10g of non-polar macroporous adsorption resin such as D201, D4020, D4006, D3520, H1020, H103, H107, HPD- to each 100ml hydroquinone aqueous solution. 100. PS, NKA, X-5 or YWD01B for adsorption; filter the adsorbed resin with filter paper, and then wash the hydroquinone solution on the resin surface with deionized water to obtain an adsorption capacity of 1g hydroquinone / g Resin immobilized hydroquinone.

[0043] (2) Fermentation of Xanthomonas campestris CGMCC NO.1243:

[0044] Pick a ring of Xanthomonas campestris CGMCC NO.1243 strain preserved on a slant, add it to a liquid medium with a volume of 250ml, and cultivate it in a shaker with a temperature of 30°C and a rotating speed of 100r / min for 15h After that, the first-level seed solution is obtained; then the first-level seed solution is inoculated into a fe...

Example Embodiment

[0053] Example 2

[0054] (1) Preparation of immobilized hydroquinone:

[0055] First prepare a hydroquinone aqueous solution with a concentration of 5% by mass; then add 10g of polar macroporous adsorption resin such as NKA-9, NKA-II, S-8, HPD-600 or YWD06 to every 100ml of hydroquinone aqueous solution Adsorption; filter the adsorbed resin with filter paper, and then wash the hydroquinone solution on the resin surface with deionized water to prepare immobilized hydroquinone with an adsorption capacity of 0.5 g hydroquinone / g resin.

[0056] (2) Fermentation of Xanthomonas campestris CGMCC NO.1243:

[0057] Pick a ring of Xanthomonas campestris CGMCC NO.1243 strain preserved on a slant, add it to a liquid medium with a volume of 250ml, and cultivate for 10h in a shaker at a temperature of 30℃ and a rotating speed of 200r / min A first-level seed liquid is obtained; then the first-level seed liquid is inoculated into a fermenter with a volume of 1000 liters according to the 10% inoculu...

Example Embodiment

[0066] Example 3

[0067] (1) Preparation of immobilized hydroquinone:

[0068] First prepare a hydroquinone aqueous solution with a concentration of 2% by mass; then add 10g weakly polar macroporous adsorption resin such as AB-8 or YWD03F to each 100ml hydroquinone aqueous solution for adsorption; filter the adsorbed resin with filter paper , And then wash the hydroquinone solution on the resin surface with deionized water to prepare immobilized hydroquinone with an adsorption capacity of 0.2g hydroquinone / g resin.

[0069] (2) Fermentation of Xanthomonas campestris CGMCC NO.1243:

[0070] Pick a ring of Xanthomonas campestris CGMCC NO.1243 strain preserved on a slant, add it to a liquid medium with a volume of 250ml, and cultivate for 10h in a shaker at a temperature of 30°C and a rotating speed of 150r / min The first-level seed liquid is obtained; the first-level seed liquid is inoculated into a fermenter with a volume of 1000 liters according to the inoculum of 2%, and cultivated ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method for synthesizing alpha-arbutin through immobilizing hydroquinone by a macro-porous adsorption resin and carrying out fermentation catalysis of the immobilized hydroquinone and disaccharide by Xanthomonas campestris having a preservation number of CGMCC NO.1243. The method comprises the steps of immobilized hydroquinone preparation, microbial fermentation, reaction condition optimization, and product separation and extraction. The alpha-arbutin output of the method is 20-235g each liter of a broth, and is 14 times higher than the output of alpha-arbutin obtained by directly using hydroquinone as a reactant, the hydroquinone conversion rate of the method reaches 90.5-95.2%, and the purity of the above obtained separated and purified product can reach above 99.5%. The immobilization of the hydroquinone improves the tolerance of a thallus to hydroquinone, simplifies the subsequent separating and purifying technology of the product, and reduces the phenol-containing wastewater emission of the above system; and the method has the advantages of high output, simple production technology, low production cost, environmental protection, and substantial reduction of the alpha-arbutin production cost, and lays a foundation for the large-scale low-cost production of alpha-arbutin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the use of macroporous adsorption resin to immobilize hydroquinone, and to synthesize α-bear from xanthomonas campestris (Xanthomonas campestris) CGMCC NO.1243 fermentation catalyzed immobilized hydroquinone and disaccharides The preparation method of fruit glycosides. Background technique [0002] α-Arbutin has the activity of significantly inhibiting tyrosinase, can reduce the deposition of tyrosinase in the skin, and has the effects of bleaching, preventing discoloration and freckle on the skin. When comparing the whitening effect of α-arbutin and its isomer β-arbutin, Kazuhisa Sugimotod et al. found that the whitening effect of α-arbutin was β-arbutin through experiments on human melanoma cells. more than 10 times. At present, α-Arbutin is an efficient and safe whitening agent recognized internationally and is being vigorously promoted. It is an ideal additive ingredient for whit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/44C12N1/20C07H15/203C07H1/06C12R1/64
Inventor 王芳刘春巧邓利张鹏张淑荣谭天伟聂开立刘珞
Owner BEIJING UNIV OF CHEM TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap