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Method for extracting DHA (Docosahexaenoic acid) from marine microalgae fermentation liquor

A technology of marine microalgae and fermentation broth, which is applied in the separation/purification of carboxylic acid compounds, fat production, fat oil/fat production, etc., and can solve problems such as toxicity, low extraction efficiency, and poor separation effect

Active Publication Date: 2014-05-14
QINGDAO LANGYATAI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the low-temperature classification method requires large-scale refrigeration equipment, high energy consumption, and low extraction efficiency; the urea inclusion method is a relatively simple and effective separation method, but when used in actual production, there are large solvent losses, drainage and urea addition. The waste treatment and other problems caused by substances; the supercritical gas extraction method can separate high-purity DHA, but the separation effect on fatty acids with the same carbon number and different double bond numbers is poor, and the equipment investment is large, the technical requirements are high, and the consumption is high. The energy is also large; the organic solvent extraction method is the microalgae oil extraction method widely used at home and abroad at present, but most of the solvents used are flammable, and the solvent vapor has certain toxicity. In addition, there are solvents in the oil extracted by the solvent method, so its poor product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Adjust the temperature of 1L of DHA microalgae fermentation liquid (initial total ester content: 9.7%) after fermentation to 50°C and pH 8.0, add alkaline protease according to 1% of the dry matter of the fermentation liquid to carry out cell wall breaking pretreatment, stir to break After 9 hours, the wall-breaking rate of the wall is 90%; according to the volume of the fermentation broth as 100%, add 1L of 95% alcohol and mix; use a centrifuge at 3000rpm to remove water, and get broken cells and algae oil after centrifuging for 10 minutes; The algae oil and cell fragments are separated by a high-speed centrifuge at 10,000 rpm to remove solid residues such as broken cells, and centrifuged for 10 minutes to obtain DHA crude oil; then the DHA crude oil is refined and purified, and after degumming, deacidification, decolorization, deodorization and other processes, finally Obtain 80g of DHA algae oil product, and the total ester extraction rate is 82.5%.

Embodiment 2

[0030] Adjust the temperature to 57°C and pH 8.4 of 1L of the fermented DHA microalgae fermentation liquid (initial total ester content is 9.7%), add alkaline protease at 0.5% of the dry matter of the fermentation liquid to carry out cell wall pretreatment, and stir to break the cell wall. After 7 hours, the wall-breaking rate of the wall is 100%; according to the volume of the fermentation broth as 90%, add 1L of 85% alcohol and mix; use a centrifuge at 3000rpm to remove water by centrifugation, and get broken cells and algae oil after centrifugation for 5 minutes; The algae oil and cell debris were separated by a high-speed centrifuge at 10,000 rpm to remove solid residues such as broken cells, and centrifuged for 5 minutes to obtain DHA crude oil. Then the DHA crude oil was refined and purified, and after degumming, deacidification, decolorization, deodorization and other processes, finally 78g of DHA algae oil was obtained, and the total ester extraction rate was 80.4%.

Embodiment 3

[0032] Adjust the temperature of 1L of DHA microalgae fermentation broth (initial total ester content is 10.2) to 60°C and pH 8.4 after fermentation, add alkaline protease at 0.5% of the dry matter of the fermentation broth for cell wall pretreatment, and stir to break the wall After 8 hours, the wall-breaking rate was 100% under microscope; according to the volume of the fermentation broth as 100%, add 1 L of 95% ethanol to mix; use a centrifuge at 4000 rpm to remove water, and get broken cells and algae oil after centrifuging for 10 minutes; The algae oil and cell fragments are separated by a high-speed centrifuge at 12000rpm to remove solid residues such as broken cells, and centrifuged for 10 minutes to obtain DHA crude oil; then the DHA crude oil is refined and purified, and after degumming, deacidification, decolorization, deodorization and other processes, the final product is obtained The DHA algae oil product is 85.6g, and the total ester extraction rate is 83.9%.

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Abstract

The invention discloses a method for extracting DHA from a marine microalgae fermentation liquor, and belongs to the technical field of extraction of microbial oil and the method can be used for solving the problems that the DHA extraction rate is low and the product is poor in quality in the prior art. The method disclosed by the invention is used for extracting the DHA by using the technical steps such as an enzyme-method wall breaking treatment, extraction of crude oil through centrifugal separation, refining and purification, wherein by virtue of the enzyme-method wall breaking treatment, the cell-wall breaking ratio can be increased and the DHA contained in cells can be fully released, so that the DHA extraction rate is greatly increased, loss and waste are reduced, and the production cost is lowered; in the centrifugal separation step, other harmful substances are not added, so that the quality of the DHA is guaranteed; by virtue of the refining step and the purification step, the purity and quality of a DHA finished product can be further improved. The method disclosed by the invention can be used for increasing the extraction rate of the DHA, the total extraction rate is over 80%, waste is reduced, the production cost is lowered, the quality of DHA algae oil is also improved, and the product performance is safe.

Description

technical field [0001] The invention relates to the technical field of extracting microbial oils, in particular to a method for extracting DHA from marine microalgae fermentation broth. Background technique [0002] DHA, the full name of docosahexaenoic acid, commonly known as brain gold, is a polyunsaturated fatty acid that is very important to the human body, and is an important member of the Omega-3 unsaturated fatty acid family. DHA is a main element for the growth and maintenance of nervous system cells. It is an important component of the brain and retina. Its content in the human cerebral cortex is as high as 20%, and it accounts for the largest proportion in the retina of the eye, accounting for about 50%. Therefore, for Intellectual and vision development of the fetus is very important. The main functions of DHA: promote fetal brain development, promote the maturation of retinal photoreceptor cells, promote the development of brain cells, be used for cancer treatme...

Claims

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Application Information

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IPC IPC(8): C07C57/03C07C51/42
CPCC11B1/00C11B1/025
Inventor 崔球李悦明宋晓金徐建春李霞夏修峦王道会
Owner QINGDAO LANGYATAI GRP
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