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A blood glucose detection method and detection kit

A detection method and technology for glucose, applied in the field of medical diagnostic preparations, can solve the problems of shortened reaction time, interference, long reaction time, etc., and achieve the effects of wide application range, simple operation and improved accuracy

Active Publication Date: 2016-06-29
潍坊泽成生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a detection method of human serum blood glucose by enzymatic rate method, which is used to quantitatively measure the concentration of blood glucose in human serum and plasma in vitro, and overcomes the susceptibility of existing detection methods to the above deficiencies. Due to the defects of serum background interference and long reaction time, the detection method of the present invention adopts the enzyme catalysis method and innovatively uses the enzymatic rate measurement method, which completely eliminates the interference of serum background, greatly shortens the reaction time, is easy to operate, and the result is accurate and reliable. Applicable to various biochemical analysis instruments

Method used

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  • A blood glucose detection method and detection kit
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  • A blood glucose detection method and detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1, a detection kit, including a packaging box, a buffer, an enzyme reagent, and a standard solution; the packaging box is the outer packaging of the kit; the buffer solution is hereinafter referred to as reagent 1, and the enzyme reagent is referred to as reagent 2.

[0038] The buffer was 0.1 mol / L phosphate buffer containing 25 mmol / L paraben.

[0039] The enzyme reagent is glucose oxidase and peroxidase solution, the concentration of glucose oxidase is 5-200U / ml; the concentration of peroxidase is 2-30U / ml.

[0040] The volume ratio of reagent 1 to reagent 2 is 5-1:1.

[0041] The standard solution is a glucose solution with a concentration of 2-7 mmol / L, which is calibrated with the national standard value assignment.

[0042] The storage conditions and validity period are: the original reagents are stored at 2-8°C, and the validity period is 12 months; after the buffer solution and enzyme reagent are made into working solution, they can be stable at roo...

Embodiment 2

[0062] Embodiment 2, a detection kit, except that the following is different from Example 1, all the other are the same as the detection kit in Example 1; the buffer is 0.1mol / L phosphate buffer, containing 25mmol / L p-hydroxybenzene Formate, the pH value is 7.3; the enzyme reagent is glucose oxidase solution, the concentration is 10U / ml, containing 5U / ml peroxidase and 3mmol / L 4-aminoantipyrine; the volume of reagent 1 and reagent 2 The ratio is 4:1; the standard solution is a blood glucose solution with a concentration of 5.55mmol / L, which is calibrated with the national standard.

[0063] Use the above detection kit to detect blood glucose. The detection method is carried out according to the following steps: the absorbance rise rate measurement step is carried out according to the double reagent mode operation. First, take the required blank tube, standard tube and sample tube according to the measurement, and add buffer respectively. ; Then add the sample to be tested, dis...

Embodiment 3

[0079] Embodiment 3, a detection kit, except that the following is different from Example 1, all the other are the same as the detection kit in Example 1; the buffer is 0.1mol / L phosphate buffer, containing 40mmol / L p-hydroxybenzene Formate, pH value is 7.3; Enzyme reagent is glucose oxidase solution, concentration is 12U / ml, contains the 4-aminoantipyrine of 2U / ml peroxidase and 1.2mmol / L; Reagent 1 and reagent 2 The volume ratio is 1:1; the standard solution is blood glucose solution with a concentration of 5.55mmol / L, which is calibrated with the national standard.

[0080] Use the above detection kit to detect blood glucose. The detection method is carried out according to the following steps: the absorbance rise rate measurement step is carried out according to the double reagent mode operation. First, take the required blank tube, standard tube and sample tube according to the measurement, and add buffer respectively. ; Then add the sample to be tested, distilled water a...

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Abstract

The invention discloses a blood glucose detection method. The method comprises the following step: by adopting an enzyme rate measurement method, measuring the absorbance rise rate within 30-180 seconds after delay of 30-60 seconds according to the relationship that the absorbance rise rate in a reaction system is in direct proportion to the concentration of blood glucose in a sample at the wavelength of 500nm, thus obtaining the content of the blood glucose. The reaction rate is measured by using the enzyme rate measurement method, reading is performed in a linear phase of the reaction process, interference of serum sample background can be completely eliminated, and the result accuracy is improved. The delay time is short, and the reading time is also short, so that the whole reaction time is greatly shortened to be within 3 minutes from the original 5-15 minutes, and the efficiency is improved.

Description

technical field [0001] The invention relates to a method for detecting blood glucose, in particular to a method and a detection kit for detecting human serum blood glucose by enzymatic rate method, which are used for quantitatively measuring the concentration of blood glucose in human serum and plasma in vitro, belonging to The technical field of medical diagnostic preparations. Background technique [0002] Under normal circumstances, the decomposition and anabolism of sugar in the human body are in a dynamic balance and remain relatively constant. Serum glucose refers to the glucose concentration in the blood. Generally, venous blood is drawn on an empty stomach after fasting for 8 to 12 hours, and the specimen is sent for inspection within 1 hour. Blood glucose measurement is one of the most important inspection items for the diagnosis of diabetes. [0003] The normal reference value is 3.9-6.1 mmol / L for adults and 2.8-4.5 mmol / L for full-term neonates. Analysis of abn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/66G01N21/31
CPCC12Q1/26C12Q1/54
Inventor 孙希武郝树彬王春光彭新国王永庆
Owner 潍坊泽成生物技术有限公司
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