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Compound probiotic micro-ecological feed additive and preparation method thereof

A compound probiotic and micro-ecological technology, applied in the field of livestock and poultry feed, can solve problems such as the decline of feed safety, and achieve the effects of improving micro-ecological balance, enhancing immunity, and improving the quality of animal products

Inactive Publication Date: 2014-06-25
吉林省泰新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of a large number of antibiotics in feed makes the safety of feed greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] 1) Preparation of probiotic starter

[0023] ① Preparation of Bacillus

[0024] Streak culture the frozen Bacillus, isolate typical colonies and inoculate them in liquid culture medium at 37°C, and culture them with shaking for 18 hours. Microscopic examination conforms to the standard form of Bacillus, and the growth state is good. The inoculation amount is 3-6% and inoculated in The liquid medium was activated, and cultured with shaking at 37°C for 18-24 hours and stored for later use.

[0025] ② Preparation of Kefir starter

[0026] Use skim milk powder to prepare 10% skim milk solution, after sterilizing at 90-115°C for 10-30 minutes, cool to 20-30°C, inoculate kefir grains according to 0.1%-0.5% of the volume of skim milk, at 23 Ferment at ~28°C for 14-18 hours and store for later use.

[0027] 2) Mixed fermentation

[0028] Take concentrated whey protein 10-20g, glucose 6-10g, yeast powder 2.5-5g, calcium carbonate 2-5g, magnesium sulfate 0.05-0.3g, manganese ...

Embodiment 1

[0033] 1) Preparation of probiotic starter

[0034] ① Preparation of Bacillus

[0035] Streak culture the frozen Bacillus subtilis, isolate typical colonies and inoculate them in liquid medium at 37°C, and culture them with shaking for 18 hours. Microscopic examination conforms to the standard form of Bacillus subtilis, and the growth state is good. The inoculum is inoculated at 3% The liquid medium was activated, and cultured with shaking at 37°C for 24 hours and stored for later use.

[0036] ② Preparation of Kefir starter

[0037] Use skim milk powder to make 10% skim milk solution, after sterilizing at 115°C for 30 minutes, cool to 25°C, inoculate kefir direct-throwing starter at 0.1% of the volume of skim milk, and ferment at 23°C Save for later use after 18 hours.

[0038] 2) Mixed fermentation

[0039] Take 10g of concentrated whey protein, 6.7g of glucose, 3.8g of yeast powder, 3.4g of calcium carbonate, 0.2g of magnesium sulfate, 0.015g of manganese sulfate, and 1...

Embodiment 2

[0044] 1) Preparation of probiotic starter

[0045] ① Preparation of Bacillus

[0046] Streak culture the frozen Bacillus subtilis and Bacillus natto, isolate the typical colonies, inoculate them in liquid culture medium at 37°C, and culture them with shaking for 18 hours. Microscopic examination meets the standards of Bacillus subtilis and Bacillus natto The morphology and growth state are good, respectively inoculate 6% of the inoculum in liquid medium for activation, and culture with shaking at 37°C for 20 hours. Mix Bacillus subtilis and Bacillus natto at a volume ratio of 2:1, and store for future use.

[0047] ② Preparation of Kefir starter

[0048] Use skim milk powder to make 10% skim milk solution, after sterilizing at 90°C for 20 minutes, cool to 23°C, inoculate kefir direct-injection starter at 0.3% of the volume of skim milk, and ferment at 26°C Save for later use after 16 hours.

[0049] 2) Mixed fermentation

[0050] Take concentrated whey protein 18g, gluco...

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Abstract

The invention relates to a compound probiotic micro-ecological feed additive and a preparation method thereof, and belongs to the field of livestock feed. The compound probiotic micro-ecological feed additive is mainly and technically characterized in that the compound probiotic micro-ecological feed additive is prepared by inoculating a culture medium with a Kefir fermentation agent, bacillus and beer yeast and carrying out fixed fermentation, low-temperature concentration and drying. In the mixed fermentation step, the Kefir fermentation agent, the beer yeast and the activated bacillus are mixed and inoculated and then fermentation is carried out for 18-24 hours under a condition at a temperature of 28-36 DEG C; a ratio of the Kefir fermentation agent to the bacillus is (1-3):1 in the mixed culture; the inoculation amount of the beer yeast is 0.1%-0.5%. The compound probiotic micro-ecological feed additive prepared by the preparation method is rich in a variety of active probiotics, high in content of viable bacteria, stable in storage and capable of improving the utilization rate of the feed, promoting the growth of the animal, significantly improving microecological balance of the animal intestinal tract, maintaining the animal intestinal health, improving the immunity of the animal, reducing the morbidity of the animal and improving the quality of the animal product.

Description

technical field [0001] The invention relates to a compound probiotic micro-ecological feed additive rich in various active probiotics and a preparation method thereof, belonging to the field of livestock and poultry feed. Background technique [0002] With the increasing level of economic development, my country's animal husbandry is also developing rapidly, and people have put forward higher requirements for healthy and safe animal products. However, the use of a large number of antibiotics in feed makes the safety of feed greatly reduced. [0003] In recent years, probiotics have gradually become an important feed additive to replace antibiotics in animal breeding. Compared with traditional feed additives, probiotic microecological preparations can effectively inhibit the growth and reproduction of pathogenic bacteria and pathogenic microorganisms in the digestive tract, and make probiotics colonize in the intestinal tract, adjust the intestinal balance of animals, and pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/00
Inventor 张铁华张欣宇田木钟姝凝姜楠
Owner 吉林省泰新生物科技有限公司
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