Method for quickly analyzing total diketone content of beer semi-finished product

A rapid analysis technology of combined diketones, applied in the measurement of color/spectral characteristics, etc., can solve the problems of excessive diacetyl in finished wine, high cost of chromatographic instruments, low detection value, etc., and achieve low safety and toxicity, fast detection speed, The effect of improving the recovery rate

Active Publication Date: 2014-06-25
东莞市珠江啤酒有限公司
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The national standard method is used to detect the total diketones of semi-finished products. As a result, during the process of sterilization and storage of beer packaging, the precursors in the wine body are slowly converted into diacetyl, resulting in excessive diacetyl in the finished wine; due to the high cost of gas chromatography, only a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly analyzing total diketone content of beer semi-finished product
  • Method for quickly analyzing total diketone content of beer semi-finished product
  • Method for quickly analyzing total diketone content of beer semi-finished product

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1

[0039] 1. Sample

[0040] 1) The source of the sample to be tested: fermentation broth-fermentation broth for beer production or fermentation broth from laboratory fermentation bottles / tubes;

[0041] 2) Sample pretreatment

[0042] If there are turbid samples such as yeast, use filter paper or centrifuge.

[0043] 2. Detection and analysis method

[0044] 1) Preparation of 5% hydrogen peroxide solution: Use a 20ml graduated pipette to accurately draw 16.7ml of commercially available 30% (m / m) hydrogen peroxide to a 100ml brown volumetric flask, and distilled water to the mark; the solution is required Store in the refrigerator at low temperature for one week.

[0045] 2) Take 100ml of fermentation broth supernatant in a 500ml long-necked flat-bottomed flask 30 with a graduated cylinder. If the fermentation broth is obviously turbid, it needs to be clarified by centrifugation or filtration. Use a pipette with a range of 10ul-100ul to accurately add 5% 100ul of hydrog...

Example Embodiment

[0066] Example 2

[0067] 1. Sample

[0068] 1) The source of the sample to be tested: fermentation broth-fermentation broth for beer production or fermentation broth from laboratory fermentation bottles / tubes;

[0069] 2) Sample pretreatment

[0070] If there are turbid samples such as yeast, use filter paper or centrifuge.

[0071] 2. Detection and analysis method

[0072] 1) Preparation of 0.2% peracetic acid solution: Use a pipette with a range of 1000ul-5000ul to accurately draw 1.33ml of commercially available 15% (m / m) peracetic acid into a 100ml brown volumetric flask, and distilled water to the mark . Both solutions need to be stored in the refrigerator at low temperature and can be stored for one week.

[0073] 2) Take 100ml of the fermentation broth supernatant in a 500ml long-necked flat-bottomed flask 30 with a graduated cylinder. If the fermentation broth is obviously turbid, it needs to be clarified by centrifugation or filtration. Use a pipette with a range of 10ul-100...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for quickly analyzing total diketone content of a beer semi-finished product. The method comprises the following steps: 1) preparing certain concentration of peroxide solution; 2) adding certain volume and certain concentration of peroxide solution to a sample before distillation; 3) distilling the sample for about 6 minutes to finish distillation by adopting a direct heating and distilling mode; 4) adding certain volume and certain concentration of hydrochloric acid solution to a 25ml distillate collecting volumetric flask before distillation, wherein the PH when the distillate is metered to the full scale is about 1.2, so that the diketone completely reacts with o-phenylenediamine to generate 2,3-dimethyl quinoxaline; and 5) carrying out colorimetric determination by using a spectrophotometer, and calculating the total diketone content. The quick analysis method disclosed by the invention has the advantages of being quick, simple to operate, convenient, accurate, high in sensitivity and the like, the variable coefficients of the repeatability and the reproducibility are smaller than 5% respectively, the recovery rate achieves over 80%, the analysis time is shortened to 30 minutes from 120 minutes in an electron beam curing (EBC) method, and the detection efficiency is improved by three times in comparison with that of the EBC method.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis of beer production, and in particular relates to a method for quickly analyzing the content of total diketones in beer semi-finished products. Background technique [0002] The total diketone content of beer includes the sum of free diacetyl and pentanedione, the precursors α-acetolactate and α-acetylhydroxybutyric acid converted to bidiketone. The content of pentanedione in beer is much lower than that of diacetyl, and its taste threshold (1mg / L) is much higher than that of diacetyl (0.1mg / L). The role is diacetyl, which is considered to be a decisive indicator to measure the maturity of beer. When the content exceeds its taste threshold of 0.10ppm, it will bring unpleasant sour rice flavor to beer. Diacetyl is the key control index of beer, which is ultimately related to the quality of the product. [0003] The premise of controlling diacetyl in finished beer is to control the t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/33
Inventor 钟晓盈涂京霞钟丽琴曾玉萍吴敏莲
Owner 东莞市珠江啤酒有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap