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Effective part of traditional Chinese medicine for treating Alzheimer disease and preparation method and application thereof

A technology for senile dementia and effective parts, applied in the field of medicine, can solve the problems of complex pathogenesis of AD and failure to achieve better therapeutic effects, and achieve good application prospects

Active Publication Date: 2014-07-02
合肥安硕医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Antipsychotic drugs are the main treatment for AD with western medicine. However, because the pathogenesis of AD is complex and involves multiple signaling pathways, although single-targeted drugs can improve or alleviate the symptoms of patients to a certain extent, they cannot achieve a good therapeutic effect.

Method used

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  • Effective part of traditional Chinese medicine for treating Alzheimer disease and preparation method and application thereof
  • Effective part of traditional Chinese medicine for treating Alzheimer disease and preparation method and application thereof
  • Effective part of traditional Chinese medicine for treating Alzheimer disease and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing an effective part of a traditional Chinese medicine for treating senile dementia, comprising the following steps:

[0032] (1) Take 6 parts of Uncaria, 4 parts of Bupleurum, 6 parts of Angelica, 6 parts of Rhizoma Chuanxiong, 8 parts of Atractylodes atractylodes, 8 parts of Poria cocos, and 3 parts of licorice, add 70% ethanol 10 times the weight of the total medicinal materials, and reflux at 80 °C Extract for 2 hours, filter the extract; then add 50% ethanol in an amount 10 times the weight of the total medicinal materials and extract at 80°C for 2 hours, and filter the extract; finally add water 10 times the weight of the total medicinal materials and extract for 2 hours at 100°C , the filtered extract; the three extracts were combined and concentrated to no alcohol smell to obtain a concentrate, i.e. the total extract extract;

[0033] (2) Get the concentrated solution obtained in step (1) on the D101 type macroporous resin column, and use the...

Embodiment 2

[0034] Example 2 The protective effect of the effective fraction group of Yigansan in the injury of PC12 cells induced by glutamate

[0035] 1 cell line

[0036] PC12 cells (PC12cell line): rat adrenal pheochromocytoma cell line (provided by the Physiological Laboratory of China Pharmaceutical University).

[0037] 2 Drugs, reagents and instruments

[0038] High-sugar DMEM dry powder medium: GIBCO Company, stored at 4°C.

[0039] Calf serum (NBCS): GIBCO company, specification: 500mL, stored at -20°C.

[0040]Penicillin: AMRESCO Company, specifications: 0242-100MU, 1500-1750U / mg, stored at 4°C.

[0041] Streptomycin: AMRESCO Company, specification: 0382-100G, 1000U / mg, stored at room temperature.

[0042] Trypsin (Trypsin 1:250): AMRESCO Company, Nanjing Boquan Technology Co., Ltd. aliquoted, specification: 10g, stored at 4°C.

[0043] MTT: Shanghai Yuanye Biotechnology Co., Ltd., specification: 1g, stored at 4°C.

[0044] Glutamic acid: Shanghai Yuanye Biotechnology Co....

Embodiment 3

[0085] The content determination of main component in the effective part YGS40 of embodiment 3

[0086] 1 Reagents and instruments

[0087] Acetonitrile (American Tiandi Company, chromatographically pure), methanol (Jiangsu Hanbang Technology Co., Ltd., chromatographically pure); experimental water was purified water; other reagents such as formic acid and ammonium acetate were produced by Nanjing Chemical Reagent Factory (analytical pure).

[0088] Shimadzu LC-2010 high performance liquid chromatography (Shimadzu Corporation), equipped with: quaternary gradient pump, ultraviolet detector, online vacuum degasser, automatic temperature control sampler, column thermostat, LC-Solution chemical workstation; Chromatographic column Hanbon Megres C18 column (4.6mm×250mm, 5μm); pHS-25 acidity meter (Shanghai Weiye Instrument Factory); KH-250DB numerical control ultrasonic cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.); BS110S electronic balance ( Beijing Sartorius Co., Lt...

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Abstract

The invention discloses an effective part of a traditional Chinese medicine for treating Alzheimer disease and a preparation method and an application thereof. The effective part of the traditional Chinese medicine for treating Alzheimer disease is prepared by a method consisting of the following steps of: taking 6 parts of gambir plant, 4 parts of radix bupleuri, 6 parts of angelica sinensis, 6 parts of ligusticum wallichii, 8 parts of rhizoma atractylodis, 8 parts of poria cocos and 3 parts of liquorice; adding alcohol or water of which the weight is 5-15 times more than the total weight of all medicine materials, and extracting for 2-4 times by refluxing, wherein each extraction is performed for 1.5-4 hours; combining extracting solutions, concentrating to be without the smell of alcohol, and thus obtaining the concentrated solution; and putting the concentrated solution on a macroporous resin column, eluting in gradient by using ethyl alcohol solutions with the ethyl alcohol concentrations of 0-95%, collecting ethyl alcohol eluent with the concentration of 30%-50%, recovering solvent, and thus obtaining the effective part of the traditional Chinese medicine. According to the preparation method of the effective part of the traditional Chinese medicine for treating Alzheimer disease, a certain separating means is utilized to separate the effective part from liver restricting powder, and a modern pharmacological experiment method is used for proving that the neuroprotective effect of the effective part is obviously superior to that of the extract of the liver restricting powder, and thus the preparation method can be used for preparing medicines for resisting Alzheimer disease.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an effective part of a traditional Chinese medicine for treating senile dementia, a preparation method and application thereof, in particular to an effective part of Yigan powder, a preparation method and application thereof. Background technique [0002] Senile dementia, also known as Alzheimer's disease (AD), is a degenerative disease of the central nervous system with an insidious onset and a chronic progressive course. Neuropsychiatric symptoms such as personality changes and language barriers seriously affect social, occupational, and life functions. The etiology and pathogenesis of AD have not yet been elucidated, and the characteristic pathological changes are extracellular senile plaques formed by the deposition of β-amyloid protein, neurofibrillary tangles formed by hyperphosphorylation of tau protein, and neuron loss with glial cell proliferation wait. [0003] Accordi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P25/28
Inventor 柳文媛刘佳赵玉荣陈涵盖亚男冯锋
Owner 合肥安硕医药科技有限公司
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