Method for preparing AuNPs-PDMS composite micro film biosensor

A biosensor and micro-thin film technology, applied in the field of biosensors, can solve the problems of the structure processing complexity of the cantilever beam structure non-specific adsorption thin film capacitive sensor structure, and achieve the effect of high sensitivity, good repeatability and reasonable design

Active Publication Date: 2014-07-09
TAIYUAN UNIV OF TECH
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Problems solved by technology

[0004] In order to solve the main problems existing in the current surface stress biosensor, such as the non-specific adsorption of the cantilever beam structure and the processing complexity of the thin film capacitive sensor structure, the present invention provides an AuNPs-PDMS composite microfilm surface stress biosensor. Preparation

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  • Method for preparing AuNPs-PDMS composite micro film biosensor
  • Method for preparing AuNPs-PDMS composite micro film biosensor
  • Method for preparing AuNPs-PDMS composite micro film biosensor

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Embodiment Construction

[0030] Specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0031] A method for preparing an AuNPs-PDMS composite microfilm surface stress biosensor, comprising the steps of:

[0032] (1) Clean the glass sheet 2, and use TMCS to silanize the glass sheet; the specific method is as follows:

[0033] Prepare a glass slide with a size of 1.3cm×1.3cm. Prepare the cleaning solution according to the volume ratio (concentrated sulfuric acid: hydrogen peroxide = 4:1), and put the glass piece in. Use a cleaning machine to wash at room temperature for 15 minutes. After cleaning, rinse the glass piece with deionized water and dry it with nitrogen. Prepare the silylating reagent TMCS, put the glass slide into a petri dish, drop a certain amount of silanizing reagent, cover the petri dish, rinse with deionized water after 20 minutes, and blow dry with nitrogen. At this point, the silanization of the glass sheet i...

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Abstract

The invention discloses a method for preparing AuNPs (nanogold)-PDMS (polydimethylsiloxane) composite micro film surface stress biosensor. The method comprises the following steps: by taking a PDMS film as a substrate and using a chloroauric acid solution as a first-step reducing agent, reducing to generate a gold seed; by utilizing the gold seed generated on the film and taking glucose and potassium bicarbonate as auxiliary reducing agents, performing second-step reduction on the composite film by using the chloroauric acid; by utilizing an AuNPs-PDMS composite film as a sensitive element of the sensor and taking PDMS as a substrate, preparing a gold electrode of the sensor by adopting a sputtering technology, and finishing preparation of the sensor. The electric conductivity of the AuNPs-PDMS composite film generated by the method is obviously improved, so that the sensitivity of the sensor is improved. Moreover, the preparation method is simple, and possibility is provided for miniature, low-cost batch production.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to a method for preparing an AuNPs-PDMS composite microfilm surface stress biosensor. Background technique [0002] As a new type of sensor, surface stress biosensors convert the binding energy of intermolecular chemical bonds into measurable optical, electrical or mechanical signals, and then realize sensing and detection. This method can detect and analyze analytes from the molecular weight level, and has higher detection and analysis accuracy, which provides a good technical support for the realization of high-precision disease detection, food pesticide or heavy metal pollution detection, and biochemical pollution detection. [0003] The sensitive element of the sensor plays an important role in the whole sensor system. Currently, microcantilever beams and microfilms are two sensitive components based on surface stress biosensors. Among them, the micro-cantilever beam surface stress b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/04
Inventor 桑胜波张文栋张丹菅傲群段倩倩马文哲李朋伟胡杰李刚
Owner TAIYUAN UNIV OF TECH
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