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Pseudomonasalcaliphila AD-28 and application thereof to denitrification of water body

A technology of Pseudomonas alkalophilus and AD-28, which is applied to the treatment of bacteria, water pollutants, and animal husbandry wastewater to achieve good denitrification effect

Active Publication Date: 2014-07-16
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of the existing biological denitrification technology for polluted water bodies, aiming to develop a denitrification bacterium to provide a high-efficiency, safe and practical biological denitrification technology for the majority of farmers

Method used

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  • Pseudomonasalcaliphila AD-28 and application thereof to denitrification of water body
  • Pseudomonasalcaliphila AD-28 and application thereof to denitrification of water body
  • Pseudomonasalcaliphila AD-28 and application thereof to denitrification of water body

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Isolation, screening and identification of strains

[0036] (1) Strain isolation:

[0037] Sample collection: In December 2012, samples of bottom sediment from fish and shrimp breeding ponds in Xiqing District, Tianjin were collected.

[0038] Sample enrichment: configure liquid enrichment medium:

[0039] DM medium: 5 g sodium citrate, (NH 4 ) 2 SO 4 2g, KH 2 PO 4 1 g, MgSO 4 ·7H 2 O 0.2 g, distilled water 1000 ml, pH7.2. Take 3-5 g of the sediment sample and add it to the enrichment medium, and incubate at 30°C for 5 days.

[0040] Separation medium: add 12-15g agar powder to DM ammonia nitrogen medium, and pour the plate to separate.

[0041] The enriched bacterial liquid was streaked and separated on the separation medium, and a single strain was obtained after 4-5 times of purification.

[0042] (2) Screening of excellent strains:

[0043] Screening medium: sodium citrate 5 g, KH 2 PO 4 1 g, MgSO 4 ·7H 2O 0.2 g, deionized water 1L, pH7.2

[0044...

Embodiment 2

[0055] Nitrogen removal effect of AD-28 strain in simulated aquaculture wastewater

[0056] The Removal Effect of AD-28 Strain on Nitrate Nitrogen

[0057] Based on the DM liquid medium, adjust the initial concentration of nitrate nitrogen to 100-400mg / L. The experimental container is a 250mL Erlenmeyer flask, with 100mL medium in each bottle, and sterilize it in an autoclave at 121°C for 20 minutes. The inoculation volume was 1%. Regularly measure the nitrate nitrogen content. The result is as figure 1 .

[0058] Depend on figure 1 It can be seen that within the concentration range of the initial concentration of nitrate nitrogen of 100-400 mg / L, the concentration of nitrate nitrogen in each treatment decreased significantly after 24 h of cultivation. When the initial concentration was 100 mg / L, the removal rate of nitrate nitrogen reached 99%, which was basically completely removed; and when the initial concentration was 200, 300, and 400 mg / L, the removal rate of nit...

Embodiment 3

[0069] Nitrogen removal effect of AD-28 strain at low temperature

[0070] Based on the DM liquid medium, adjust the initial concentration of nitrate nitrogen to 128 mg / L, the experimental container is a 250mL Erlenmeyer flask, each bottle contains 100mL medium, sterilize in an autoclave at 121°C for 20 min, and inoculate The amount of 1% was cultured at 10°C, 15°C, 20°C, 25°C, 30°C, and 35°C respectively, and the content of nitrate nitrogen was measured regularly. The result is as Figure 5 .

[0071] Depend on Figure 5 It can be seen that the AD-28 strain at 20-35 °C can remove 99% of the nitrate nitrogen with an initial concentration of 128 mg / L within 48 h, and the AD-28 strain at 15 °C can remove nitrate nitrogen with an initial concentration of 128 mg / L within 72 h. The nitrate nitrogen removal rate of L reaches 99%.

[0072] Based on DM liquid medium, the initial concentration of nitrite nitrogen was adjusted to 13 mg / L, and the experimental method was the same as ...

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Abstract

The invention discloses Pseudomonasalcaliphila AD-28 for rapid denitrification of a cultivation water body, and the preservation number of the Pseudomonasalcaliphila AD-28 is CGMCC No.8991. The Pseudomonasalcaliphila disclosed by the invention can be used for rapidly removing nitrate nitrogen, nitrite nitrogen and ammonia nitrogen from the water body so as to improve the environment of the water body and reduce harms caused by nitrogen-containing pollutants to the organisms in the water body, thus an efficient, safe and practical biological denitrification technology is provided.

Description

technical field [0001] The invention belongs to the technical field of biological bacteria preparation and the technical field of environmental microorganism application. It involves a strain of alkalophilic Pseudomonas AD-28 and its application in water denitrification. Background technique [0002] According to the State Environmental Protection Administration’s 2010 Bulletin on the State of the Environment in China, the main lakes in my country are heavily polluted with nitrogen and phosphorus. Due to the increase in breeding density and excessive feed input, the problem of eutrophication in aquaculture water is prominent. High concentrations of ammonia nitrogen and (sub)nitrate nitrogen not only have a direct toxic effect on many aquatic animals, but also reduce their immunity, resulting in frequent occurrence of aquatic diseases and bringing huge losses to the aquaculture industry. Therefore, to solve the problem of eutrophication of water body faster and better has be...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38C02F103/20
Inventor 张峰峰谢凤行周可孙海波赵玉洁
Owner 天津市农业科学院
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