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Preparation method and application of molecular imprinting sensor for detecting apigenin

A molecular imprinting and apigenin technology, applied in the direction of electrochemical variables of materials, can solve the problems of poor regeneration and reversibility, high detection limit, affecting the application of molecular imprinting technology, etc., to achieve improved response, high affinity and selectivity. Effect

Inactive Publication Date: 2014-07-16
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of oxidized carbon nanotubes: In the reactor, add 22mL concentrated sulfuric acid, 0.5g carbon nanotubes, 0.5g sodium nitrate, 3.0g potassium permanganate, ultrasonic dispersion for 80min, slowly add 40mL double distilled water, Stir and react at 85°C for 5 h, cool to room temperature, filter, wash with 1 mol / L HCl, and then repeatedly wash with double distilled water until the filtrate is neutral, dry to obtain carbon dioxide nanotubes;

[0019] (2) Preparation of carbon nanotube-modified glassy carbon electrodes: the glassy carbon electrodes were sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with twice distilled water, dried with nitrogen, and 6 μL of N,N-dimethylformamide dispersion (0.5 g / L) of carbon oxide nanotubes was dropped on the surface of the glassy carbon electrode, and placed After evaporating the solvent under infrared light, the carbon nanotube-modified glassy carbon...

Embodiment 2

[0023] (1) Preparation of oxidized carbon nanotubes: In the reactor, add 10mL concentrated sulfuric acid, 0.5g carbon nanotubes, 1.0g sodium nitrate, 2.0g potassium permanganate, ultrasonically disperse for 90min, slowly add 40mL double distilled water, Stir and react at 90°C for 4 h, cool to room temperature, filter, wash with 1 mol / L HCl, and then repeatedly wash with double distilled water until the filtrate is neutral, dry to obtain carbon dioxide nanotubes;

[0024] (2) Preparation of carbon nanotube-modified glassy carbon electrodes: the glassy carbon electrodes were sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with double-distilled water, dried with nitrogen, and 4 μL of N,N-dimethylformamide dispersion (0.5 g / L) of carbon oxide nanotubes was dropped on the surface of the glassy carbon electrode. After evaporating the solvent under infrared light, the carbon nanotube-modified glassy carbon electro...

Embodiment 3

[0028] (1) Preparation of oxidized carbon nanotubes: In the reactor, add 18mL concentrated sulfuric acid, 1.0g carbon nanotubes, 0.5g sodium nitrate, 0.6g potassium permanganate, ultrasonically disperse for 60min, slowly add 20mL double distilled water, Stir the reaction at 80°C for 6 hours, cool to room temperature, filter, wash with 1 mol / L HCl, and then repeatedly wash with twice distilled water until the filtrate is neutral, dry to obtain carbon dioxide nanotubes;

[0029] (2) Preparation of carbon nanotube-modified glassy carbon electrodes: the glassy carbon electrodes were sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with twice distilled water, dried with nitrogen, and 5 μL of N,N-dimethylformamide dispersion (0.5 g / L) of carbon oxide nanotubes was added dropwise on the surface of the glassy carbon electrode. After evaporating the solvent under infrared light, the carbon nanotube-modified glassy ca...

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Abstract

The invention discloses a preparation method of a molecular imprinting sensor for detecting apigenin. The preparation method is characterized by firstly modifying a glassy carbon electrode with a carbon nanotube, adding the following components to a reactor according to the mass percentage concentration: 10-30% of ethylene glycol dimethacrylate, 2.0-12% of itaconic acid, 54-80% of absolute ethyl alcohol, 1.0-6% of azodiisobutyronitrile and 0.1-2.5% of apigenin, stirring the components at 60-70 DEG C in the atmosphere of argon to react for 30-36 hours, soaking the obtained product with a mixed solution of methanol and acetic acid for 6-8 hours, removing template molecules and drying the product, thus obtaining an apigenin molecularly imprinted polymer. The imprinting sensor shows relatively high affinity and selectivity towards apigenin. A sensor capable of specially recognizing template molecules is formed by connecting the apigenin molecular imprinting sensor with an electrochemical workstation. The sensor prepared by the preparation method is low in cost, has high sensitivity and good specificity, is quick in detection and can be reused. The response of the prepared molecular imprinting sensor to apigenin is greatly improved.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of an apigenin molecularly imprinted sensor, which is based on the specific recognition of molecular imprinting and is used to detect medicines, food, Apigenin technology in biological samples. Background technique [0002] Apigenin (apigenin) is a flavonoid compound, also known as apigenin and apigenin, which is widely distributed in vegetables and fruits in warm tropical regions, especially in celery; in some medicinal plants such as plantain There is also a high content in scorpion, terrazzo, etc., and it is also distributed in plant-derived beverages such as tea, wine, and some condiments. The chemical structure of apigenin is 5, 7, 4'-trihydroxyflavone, and its molecular formula is C 15 h 10 o 6 , The relative molecular mass is 270. Apigenin is insoluble in water,...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
Inventor 许崇娟李慧芝庄海燕
Owner UNIV OF JINAN
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