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Process for extracting cordycepin from Cordyceps militaris

An extraction method and technology of cordycepin, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of poor reusability, low purity of cordycepin, high production cost, etc., and achieve a reusable period Long, high product purity, short production cycle

Active Publication Date: 2015-10-21
邓华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of cordycepin obtained by this method is relatively low, generally around 92%. Although large-scale production can be realized, the process is relatively complicated, especially the use of macroporous resin as the adsorption material, which has poor reusability, resulting in high production costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 : Put the Cordyceps militaris particles crushed into 120 μm into the extraction tank, add 10Kg of Cordyceps militaris particles to every 100Kg of 85% ethanol solvent to extract 3 times, stir and heat during extraction, keep the temperature at 50°C for 60 minutes, take extract.

[0047] Combine the extracts obtained from the alcohol extraction into a falling film evaporator, suction filter and cool under a negative pressure of 0.05MPa, and heat the filtrate to recover the solvent. When the solid-liquid ratio of the concentrate is lower than 1:10, the concentrated extract is obtained.

[0048] Add petroleum ether in an amount twice the weight of the concentrated extract, put it into a 3-5 stage extraction system for extraction, control the temperature at 40-60°C, extract for 2-6 hours, filter and separate with a 38-180 μm filter to obtain the liquid phase and solid phase.

[0049] Put the obtained liquid phase into a solvent distillation tower to carry out des...

Embodiment 2

[0057] Example 2 : Put the Cordyceps militaris particles crushed into 180 μm into the extraction tank, add 8Kg of Cordyceps militaris particles to every 100Kg of 90% ethanol solvent for extraction twice, stir and heat during the extraction process, keep the temperature at 52°C, and keep warm for 120°C Minutes, take the extract.

[0058] Put the extract obtained by alcohol extraction into a falling film evaporator, suction filter and cool under 0.05MPa negative pressure, and heat the obtained filtrate to recover the solvent. When the solid-to-liquid ratio of the concentrate is 1:8, the concentrated extract is obtained.

[0059] Add petroleum ether in an amount 1.5 times the weight of the concentrated extract, put it into a three-stage extraction system for extraction, control the temperature at 60°C, extract for 3 hours, and separate to obtain a liquid phase and a solid phase.

[0060] The obtained liquid phase is placed in a solvent distillation tower for precipitation and p...

Embodiment 3

[0069] Example 3 : Take the Cordyceps militaris granules crushed into 150 μm, put them into the extraction tank, add 6Kg of Cordyceps militaris granules to every 100Kg of 90% ethanol solvent to extract 3 times, stir and heat during the extraction process, keep the temperature at 48°C, and keep warm for 100 Minutes, take the extract.

[0070] Put the extract obtained by alcohol extraction into the falling film evaporator, suction filter and cool under the negative pressure of 0.07MPa, and heat the obtained filtrate to recover the solvent. When the solid-liquid ratio of the concentrate is lower than 1:5, the concentrated extract is obtained.

[0071] Add petroleum ether in an amount 2.5 times the weight of the concentrated extract, put it into a three-stage extraction system for extraction, control the temperature at 40°C, extract for 3 hours, and separate to obtain a liquid phase and a solid phase.

[0072] The obtained liquid phase is put into a solvent distillation tower fo...

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PUM

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Abstract

The invention discloses a process method for extracting cordycepin from cordyceps militaris. The process method comprises the following steps: carrying out alcohol extraction; concentrating; dewaxing; purifying; liquid membrane extraction; refining; and drying. Compared with the prior art, the process is simplest and short in production period. According to the process, an organic solvent is small in usage amount and is reusable; high product yield is achieved; especially, the material of a liquid membrane extraction system which adopts an FEP (Fluorinated Ethylene Propylene) high-molecular polymer filler is long in reusing period and can be indefinitely reused in theory, so that the production cost can be greatly saved; and especially, the purity of a produced product is high, and the content of extracted refined cordycepin can achieve more than 98%, so that the condition is provided for large-scale industrialized production.

Description

technical field [0001] The invention relates to a method for extracting cordycepin, in particular to a process for extracting cordycepin from Cordyceps militaris. Background technique [0002] Cordycepin (cordycepin), also known as cordycepin, is the main active ingredient in Cordyceps militaris (especially nucleosides), and it is also the first nucleoside antibiotic isolated from fungi. [0003] Cordycepin is a drug of natural origin, which has various pharmacological effects such as antitumor, antibacterial and antiviral, immune regulation, and scavenging free radicals, and has a good clinical application prospect. [0004] Cordycepin research is now becoming an extremely active field in medicinal chemistry. [0005] Cordycepin is an analogue of adenosine, molecular formula: C 10 h 13 N 5 o 3 , Molecular weight: 251.24, alkaline, needle-like or flaky crystal, melting point 230℃~231℃, maximum absorption wavelength 259.0 nm, soluble in water, hot ethanol and methanol, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/16C07H1/08C08B37/00
Inventor 邓华
Owner 邓华