Trichoderma aureoviride particle adsorbent for treating uranium-bearing wastewater as well as preparation method and application of absorbent
A granular adsorbent, the technology of Trichoderma chrysogreen, applied in biological water/sewage treatment, water/sludge/sewage treatment, other chemical processes, etc., achieves the effect of simple preparation method, good adsorption effect, and good solid-liquid separation effect
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Embodiment 1
[0026] Inoculate Trichoderma chrysanthemum into PDA solid medium, and culture it statically at 28°C for 5-6d until conidia are produced, wash the surface of the thalline with 5ml sterile distilled water to make conidia suspension, adjust the concentration of spores with sterile distilled water to 10 6 pieces / ml. Wash the corn cobs with clean water, dry them in a drying oven at 60°C for 12 hours to constant weight, crush the corn cobs with a pulverizer, screen the particles with a particle size of 1-2mm, sterilize with high pressure steam at 121°C for 30 minutes, and cool at room temperature. Under sterile conditions, add 0.15g of corncob particles into a 50ml centrifuge tube, add 10ml of conidia suspension, shake and mix slightly, and let stand at room temperature for 48 hours. The adsorption amount of conidia on the surface of corncob particles is about 6×10 7 conidia / g. Transfer the corncob particles with conidia adsorbed to 10ml of sodium alginate solution with a mass fr...
Embodiment 2
[0031] Accurately pipette 50ml, 25μg / ml uranium standard solution into a 150ml Erlenmeyer flask, and adjust the pH value with 0.1mol / L HCl / NaOH, so that the pH value is between 3.28-8.63. Add 0.03g of Trichoderma chrysoderma granule adsorbent, and adsorb on a constant temperature oscillator at 28°C and 145r / min for 12h. Centrifuge at room temperature at 13000r / min for 5min, take the supernatant, and measure the concentration of uranium ions in the solution by arsenazoⅢ spectrophotometry.
[0032] The adsorption amount of uranium ions is calculated according to the following formula (the same below):
[0033] In the formula: q e - Adsorption capacity, mg / g; V - volume of uranium solution, L; C e - Equilibrium concentration of uranium ion solution, mg / L; C0 - Initial concentration of uranium ion solution, mg / L; m - mass of adsorbent, g.
[0034] The results are shown in Table 1:
[0035] Table 1 Adsorption effect of Trichoderma chrysanthemum particle adsorbent on uraniu...
Embodiment 3
[0039] Accurately pipette 50ml, 25μg / ml uranium standard solution into a 150ml Erlenmeyer flask, adjust the pH value with 0.1mol / L HCl / NaOH to make the pH value 6.23, then add 0.03g Trichoderma chrysanthemum particle adsorbent, After adsorbing on a constant temperature oscillator at 28°C and 145r / min for a certain period of time, centrifuge at room temperature at 13000r / min for 5min, take the supernatant, and measure the concentration of uranium ions in the solution by arsenazo III photometry. The results are shown in Table 2:
[0040] Table 2 Adsorption effect of Trichoderma chrysanthemum particle adsorbent on uranium ions at different adsorption times
[0041] Adsorption time (h) 0.5 1 2 3 4 6 8 10 12 24 Adsorption capacity (mg / g) 11.93 13.15 13.96 15.79 16.61 16.83 16.61 17.44 18.23 17.83
[0042] As shown in Table 2, when the adsorption time is 6h, the adsorption basically reaches equilibrium, so the optimal time for adsorption is 6h...
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