Trichoderma aureoviride particle adsorbent for treating uranium-bearing wastewater as well as preparation method and application of absorbent

A granular adsorbent, the technology of Trichoderma chrysogreen, applied in biological water/sewage treatment, water/sludge/sewage treatment, other chemical processes, etc., achieves the effect of simple preparation method, good adsorption effect, and good solid-liquid separation effect

Inactive Publication Date: 2014-07-30
EAST CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no relevant reports at home and abroad on the ability of immobiliz

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  • Trichoderma aureoviride particle adsorbent for treating uranium-bearing wastewater as well as preparation method and application of absorbent
  • Trichoderma aureoviride particle adsorbent for treating uranium-bearing wastewater as well as preparation method and application of absorbent
  • Trichoderma aureoviride particle adsorbent for treating uranium-bearing wastewater as well as preparation method and application of absorbent

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Inoculate Trichoderma chrysanthemum into PDA solid medium, and culture it statically at 28°C for 5-6d until conidia are produced, wash the surface of the thalline with 5ml sterile distilled water to make conidia suspension, adjust the concentration of spores with sterile distilled water to 10 6 pieces / ml. Wash the corn cobs with clean water, dry them in a drying oven at 60°C for 12 hours to constant weight, crush the corn cobs with a pulverizer, screen the particles with a particle size of 1-2mm, sterilize with high pressure steam at 121°C for 30 minutes, and cool at room temperature. Under sterile conditions, add 0.15g of corncob particles into a 50ml centrifuge tube, add 10ml of conidia suspension, shake and mix slightly, and let stand at room temperature for 48 hours. The adsorption amount of conidia on the surface of corncob particles is about 6×10 7 conidia / g. Transfer the corncob particles with conidia adsorbed to 10ml of sodium alginate solution with a mass fr...

Embodiment 2

[0031] Accurately pipette 50ml, 25μg / ml uranium standard solution into a 150ml Erlenmeyer flask, and adjust the pH value with 0.1mol / L HCl / NaOH, so that the pH value is between 3.28-8.63. Add 0.03g of Trichoderma chrysoderma granule adsorbent, and adsorb on a constant temperature oscillator at 28°C and 145r / min for 12h. Centrifuge at room temperature at 13000r / min for 5min, take the supernatant, and measure the concentration of uranium ions in the solution by arsenazoⅢ spectrophotometry.

[0032] The adsorption amount of uranium ions is calculated according to the following formula (the same below):

[0033] In the formula: q e - Adsorption capacity, mg / g; V - volume of uranium solution, L; C e - Equilibrium concentration of uranium ion solution, mg / L; C0 - Initial concentration of uranium ion solution, mg / L; m - mass of adsorbent, g.

[0034] The results are shown in Table 1:

[0035] Table 1 Adsorption effect of Trichoderma chrysanthemum particle adsorbent on uraniu...

Embodiment 3

[0039] Accurately pipette 50ml, 25μg / ml uranium standard solution into a 150ml Erlenmeyer flask, adjust the pH value with 0.1mol / L HCl / NaOH to make the pH value 6.23, then add 0.03g Trichoderma chrysanthemum particle adsorbent, After adsorbing on a constant temperature oscillator at 28°C and 145r / min for a certain period of time, centrifuge at room temperature at 13000r / min for 5min, take the supernatant, and measure the concentration of uranium ions in the solution by arsenazo III photometry. The results are shown in Table 2:

[0040] Table 2 Adsorption effect of Trichoderma chrysanthemum particle adsorbent on uranium ions at different adsorption times

[0041] Adsorption time (h) 0.5 1 2 3 4 6 8 10 12 24 Adsorption capacity (mg / g) 11.93 13.15 13.96 15.79 16.61 16.83 16.61 17.44 18.23 17.83

[0042] As shown in Table 2, when the adsorption time is 6h, the adsorption basically reaches equilibrium, so the optimal time for adsorption is 6h...

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Abstract

The invention relates to a trichoderma aureoviride particle adsorbent for treating uranium-bearing wastewater as well as a preparation method and application of the absorbent, and belongs to the technical field of production and manufacturing of a sewage treatment material. The adsorbent is prepared from trichoderma aureoviride fungus purely-cultured viable bacteria, corncob particles and an embedding agent and is 4mm-6mm in particle size; the preparation method comprises the following steps: culturing the trichoderma aureoviride bacteria and washing with sterile water to obtain conidium suspension liquid; washing, drying and crushing corncobs, and then sieving the particles of which the particle size is 1mm-2mm; and adsorbing, embedding, culturing and freeze-drying conidiums to obtain the trichoderma aureoviride particle adsorbent. The application is as follows: the trichoderma aureoviride particle adsorbent is added into a uranium-bearing water solution, and then the vibration and the adsorption are performed. The particle adsorbent prepared by adopting the method disclosed by the invention is low in cost, simple and convenient to operate, free of secondary pollution, liable to separate from the water solution, reusable, and capable of being used for effectively adsorbing and recycling uranium ions in the water solution.

Description

technical field [0001] The invention belongs to the field of waste water adsorbent and its preparation and application, in particular to a trichoderma chrysogreen particle adsorbent for treating uranium-containing waste water and its preparation and application. Background technique [0002] Biosorption treatment of wastewater containing heavy metals is an emerging research field. As a new technology to deal with heavy metal pollution, biosorption method has the advantages of low adsorption cost, high efficiency and no secondary pollution compared with other similar technologies such as evaporation, chemical precipitation, activated carbon adsorption, ion exchange resin electrodialysis, etc. It has broad application prospects in the treatment of heavy metal-containing wastewater. A large number of studies have shown that a variety of microorganisms such as bacteria, fungi, and algae can be used for the biosorption of heavy metal ions. Filamentous fungi such as Rhizopus ar...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30C02F3/34C12N11/12C12R1/885
Inventor 李敏张志宾马腾腾王金扣刘云海于梁梁
Owner EAST CHINA UNIV OF TECH
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