Cadmium-resistant bacteria and method for inhibiting heavy metal cadmium absorption of paddy rice
A technology of bacterial inhibition and heavy metals, applied in the field of agriculture and environmental pollution control, can solve the problems of soil structure damage, content reduction, complex equipment, etc., achieve strong repair effect, reduce cadmium content of brown rice, and seedling growth without inhibition
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Embodiment 1
[0017] Example 1: Screening of bacterial strain TCd-1
[0018] Dissolve 5 g of rice rhizosphere soil in 50 mL of sterile water, and put 150 μL of the solution in sterilized beef extract peptone liquid medium (medium: beef extract 3 g L -1 , peptone 10 g·L -1 , NaCl 5 g·L -1 , agar 20 g·L -1 , pH 7.3), shake on a shaker at 150 rpm at 37°C for 12 h. Then draw 0.1 mL from it and spread it on the -1 Five beef extract peptone solid medium plates with different cadmium concentrations were placed on each group with three replicates, and they were cultured upside down in a 37 °C incubator for 24 h. According to the growth of bacteria, take 300 mg·L -1 Streak culture was carried out on the plate medium with cadmium concentration, and the operation was repeated more than 3 times to observe the growth of the colony TCd-1, and the slant was stored in a refrigerator at 4 °C until use.
Embodiment 2
[0019] Example 2: Cadmium uptake properties of bacterial strain TCd-1
[0020] Under sterile conditions, the strain TCd-1 preserved in Example 1 was transferred to beef extract peptone liquid medium, and cultured with shaking at 150 rpm at 37°C for 12 hours was used as the seed liquid. Draw 1 mL of TCd-1 seed solution into 100 mL and the concentration of cadmium is 100 mg·kg -1 The beef extract peptone liquid medium was cultured with shaking at 37 °C for 24 h. Take 10 mL of the culture solution, centrifuge at 10000 rpm for 10 min, rinse the cell pellet with sterile water for 3 times, dry and weigh it, and use it to determine the cadmium content of the cell. After the same treatment as above, the bacterial cell pellet was suspended in 10 mL of sterile water, ultrasonically crushed for 140 s, and centrifuged at 12,000 rpm for 10 min to obtain the cell wall pellet, which was used for the determination of cadmium content in the bacterial cell wall, and the supernatant was used ...
Embodiment 3
[0023] Example 3: Restoration of rice growth under cadmium stress by bacterial strain TCd-1
[0024] The germinated rice seeds were taken and evenly implanted with 5 mg·L -1 CdCl 2 10 seeds were implanted in each petri dish, and the agarose medium implanted with no cadmium treatment was used as a control, and 5ml of distilled water (treatment Ⅰ) and bacterial solution of strain TCd-1 (treatment Ⅱ) Two treatments, each treatment repeated 5 times. Each treatment was placed in a greenhouse at 25°C for light cultivation. Water was supplemented quantitatively every day, and rice biomass was measured after 7 days. The results showed that under cadmium stress, the biomass of rice treated with TCd-1 bacterial solution was similar to that of the control. The plant height and fresh weight of rice treated with liquid solution increased by 38.4% and 38.1%, respectively, and the restoration effect was extremely significant. (As shown in table 2)
[0025] Table 2 Restoration effe...
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