ngal light-excited chemiluminescence detection kit, its preparation and use method

Abstract

The invention relates to a method for preparing an NGAL (Neutrophil Gelatinase Associated Lipocalin) optical excitation chemiluminescence detection kit. The method comprises the following steps: activating a luminous microsphere, namely activating the luminous microsphere through prepared carbodiimide and Sulfo-NHS solutions in a PBS (Phosphate Buffer Saline) buffer solution, wherein the luminous microsphere is a carboxyl modified luminous microsphere, and the luminous microsphere is coated with dimethylthiophene, anthracene and rubrene and carried with an europium chelate; coupling the luminous microsphere with an anti-NGAL antibody, namely selecting the carboxyl modified luminous microsphere, carrying out mixing reaction on the anti-NGAL monoclonal antibody and the activated carboxyl modified luminous microsphere to couple the anti-NGAL antibody to the luminous microsphere, and adding freshly prepared carbodiimide at the same time while mixing the anti-NGAL antibody and the activated luminous microsphere; labeling the anti-NGAL antibody through biotin; and labeling a photosensitive microsphere through avidin. The invention also relates to preparation and use methods of the NGAL optical excitation chemiluminescence detection kit. The methods disclosed by the invention solve the problems that the sensitivity is low and the detection is easily interfered by the environment in a detection method in the prior art.

Description

technical field

[0001] The invention relates to the field of detection of lipocalin content, in particular to a light-excited chemiluminescence detection kit for neutrophil gelatinase-associated lipocalin (NGAL) in human body, and its preparation and use methods. Background technique

[0002] Currently the most commonly used detection methods for NGAL are colloidal gold immunoassay and chemiluminescence immunoassay (CLIA);

[0003] But the applicant finds that these methods have some defects when using colloidal gold and CLIA methods: the detection sensitivity of colloidal gold is not high, and cannot be accurately quantified; there are many influencing factors of CLIA, such as being susceptible to environmental interference, short luminescence time, and being Non-open reagents, high reagent prices. Contents of the invention

[0004] In order to solve the above technical problems, the present invention provides a NGAL light-excited chemiluminescence detection kit, its pre...

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