A kind of glucose enzyme electrode based on spherical cross-scale structure array and its preparation method
A glucose enzyme and cross-scale technology, which is applied in the field of micro-sensing, can solve the problem that the cross-scale glucose enzyme electrode does not involve spherical cross-scale structure arrays, etc., and achieves the effects of improving test performance, high processing accuracy and low cost.
Inactive Publication Date: 2016-06-29
XI AN JIAOTONG UNIV
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[0005] The cross-scale glucose enzyme electrodes mentioned in the above studies did not involve arrays of spherical cross-scale structures
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[0039] Below in conjunction with accompanying drawing and embodiment case, the present invention is described in further detail:
[0040] see figure 1 , 2. The bonded gold wire is formed into a composite structure of gold microspheres and gold wire leads through the fusion method, and a silicon substrate with a specific thickness and an optical fiber core array with a specific pitch are used as longitudinal and lateral positioning fixtures respectively. 3 ×3 gold microsphere array components. The diameter of the bonding gold wire is Φ50 μm; the diameter of the gold microsphere is Φ400 μm; the length of the optical fiber core is 13 mm and the diameter is Φ125 μm; the length of the silicon substrate is 8 mm, the width is 8 mm, and the thickness is 520 μm; Synthesize ZnO nanowires on the surface of microspheres, the length of which is 1-2 μm, the end surface is a regular hexagonal structure, and the diameter of the regular hexagonal structure is Φ40-60nm; GOD with an activity of...
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Abstract
The invention discloses a glucolase electrode based on a spherical cross-scale structure array and a preparation method thereof. The preparation method comprises the following steps: smelting and molding bonded gold wires to prepare a composite composition composed of gold micro balls with a diameter phi of 400 [mu]m and gold lead wires; establishing a 3*3 gold micro ball array component on the basis of a silicon substrate with a thickness of 520 [mu]m, wherein the longitudinal pitch of the array component is 570 [mu]m, and the transversal pitch is 625 [mu]m; encapsulating the 3*3 gold micro ball array component in a plastic burette by using epoxy resin, synthesizing ZnO nano wires on the 3*3 micro ball array so as to obtain the spherical cross-scale structure array; and finally fixing glucolase on the surface of the spherical cross-scale structure array so as to obtain the glucolase electrode based on a spherical cross-scale structure array. The preparation method can high-precisely and inexpensively prepare gold micro ball arrays with special pitches in batches. The adsorption surface of the spherical cross-scale structure is large, so the glucose oxidase fixation is assisted, the catalytic performance of enzyme electrode is improved, and moreover the miniaturization of enzyme electrode is achieved.
Description
technical field [0001] The invention belongs to the field of micro-sensing technology, and is mainly used in the fields of electrochemistry, biology, medicine, food processing, environmental monitoring, etc., and particularly relates to a glucose enzyme electrode based on a spherical cross-scale structure array and a preparation method thereof, which is used for human blood and detection of glucose concentration in urine. Background technique [0002] Diabetes is an endocrine and metabolic disease, often accompanied by cardiovascular and cerebrovascular diseases, renal failure and other complications, which seriously threaten human health. The third-generation glucose sensor is mainly characterized by direct electron transfer between GOD and the substrate electrode. It has high sensitivity, fast response, low detection limit, and easy operation. It is receiving more and more attention in diabetes diagnosis and blood glucose control. . The research focus of this type of glu...
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IPC IPC(8): G01N27/327
Inventor 景蔚萱齐含成妍妍蒋庄德陈路加周帆王兵
Owner XI AN JIAOTONG UNIV