Method for clearing wild algae contamination in live history micro-phase of large economic algae

A technology of economical algae and life history, which is applied in the field of removing miscellaneous algae pollution in the microscopic stage of the life history of large economical algae. It can solve the problems of preservation pollution, death, and inhibition of target algae damage, and achieves a wide range of seaweed materials, easy separation and acquisition, and high versatility. sexual effect

Inactive Publication Date: 2014-08-27
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, because the algae in the microscopic stage of life history are very small, they are easily polluted by filamentous or unicellular cyanobacteria, diatoms and other microalgae during preservation and cultivation, which will interfere with subsequent experiments or production
Because microalgae have obvious advantages in terms of nutrient absorption and proliferation rate, if not removed in time, the target algae will often be completely wrapped and lead to death, which will seriously affect production or scientific research
[0007] Existing solutions include: 1) Re-isolation of target algae through micromanipulation, which is highly dependent on relevant equipment and operating experience, and it takes time to re-proliferate target algae from a single individual; 2) Use chemical reagents to selectively control The growth of miscellaneous algae, for example, using germanium dioxide to inhibit diatoms by interfering with silicon metabolism, however, miscellaneous algae will develop tolerance by themselves or through spores, so they often only have an inhibitory effect and are difficult to completely remove. In addition, chemical agents will also affect the target. Inhibition and damage to algae; 3) Discard directly

Method used

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  • Method for clearing wild algae contamination in live history micro-phase of large economic algae

Examples

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Effect test

Embodiment 1

[0032] Example 1: Isolation and culture of Daphnia and morphological and molecular identification

[0033] 1. Isolation and culture of flea species

[0034] The lower benthic Daphnia were collected from the intertidal zone of Qingdao sea area, rinsed with sterilized seawater and separated from two paired individuals, and maintained and multiplied by feeding benthic diatoms.

[0035] 2. Morphological observation

[0036] Pick a typical intact individual, use scanning electron microscope and optical microscope to observe the morphological characteristics and image, the electron microscope shows that the mouthparts diameter of Daphnia is about 10 μm, which is larger than the diameter of common filamentous cyanobacteria, unicellular cyanobacteria and diatoms, and smaller than common large cyanobacteria. Cell dimensions of the microscopic stages of economical seaweed. Light microscopy shows that female individuals form oocysts, which contain a large number of eggs and have a high...

Embodiment 2

[0044] Embodiment 2: The filamentous cyanobacteria that pollutes Enteromorpha seedlings are removed by daphnia

[0045] Enteromorpha seedlings can be used to preserve germplasm. Filamentous cyanobacteria are common polluting organisms, which are very prone to serious entanglement with seedlings and eventually complete encapsulation. Due to complete inhibition of photosynthesis and competition for nutrients, the preserved seedling germplasm dies.

[0046] Take seedlings that are in a long-term dormant state and have seriously polluted filamentous cyanobacteria at the rhizomes, and mix them with an appropriate amount of Daphnia (about 20) in a petri dish, and treat for about half an hour. During the whole process, microscopic examination and photographs were taken every 10 minutes (see Figure 5 ). It can be seen in the figure that the filamentous cyanobacteria are seriously entangled with the rhizoids, but the removal of the cyanobacteria by Daphnia is very thorough and effici...

Embodiment 3

[0047] Embodiment 3: Diatoms that pollute Ulva seedlings are removed by Daphnia

[0048] Ulva seedlings can be used to preserve germplasm. Diatoms are common polluting organisms, which are very easy to adhere to the seedlings. In severe cases, they will be completely encapsulated, and the germplasm of preserved seedlings will die due to complete inhibition of photosynthesis and competition for nutrients.

[0049] Take the Ulva seedlings that are in a long-term dormant state and are seriously polluted with diatoms, and mix them with an appropriate amount of Daphnia (about 20) in a petri dish, and treat them for about half an hour. During the whole process, microscopic examination and photographs were taken every 10 minutes (see Figure 6 ). It can be seen from the figure that the unicellular diatoms are seriously adhered to the seedling algae, but the diatoms are completely and efficiently removed by Daphnia.

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Abstract

The invention belongs to the field of biotechnologies of algae and relates to a method for clearing wild algae contamination in the live history micro-phase of large economic algae. According to the method, water fleas inhabiting in the lower middle of an ocean are mixed with contaminating wild algae in the live history micro-phase of large economic algae so as to remove the contaminating algae by natural feeding behaviors of the water fleas. The method has the advantages that the contaminating algae in the live history micro-phase of various economic algae can be cleared efficiently and thoroughly and no harm is caused to the economic algae.

Description

technical field [0001] The invention belongs to the technical field of seaweed biology, and relates to a method for removing miscellaneous algae pollution in the microscopic stages of the life history of large-scale economic seaweeds. Background technique [0002] Macroalgae mainly include brown seaweed, red seaweed and green seaweed. Many of these economic species are the subject of artificial cultivation, such as brown algae of kelp (Saccharina spp.), wakame (Undaria pinnatifida), hijiki (Sargassum fusiforme), red algae of laver (Pyropia spp.), red cabbage ( Bangia spp.), Gracilaria spp., Gelidium spp., Eucheuma spp., and green algae Enteromorpha and Ulva spp. [0003] Seaweed products obtained through artificial cultivation can not only provide healthy food (such as seaweed, red cabbage, kelp, wakame, enteromorpha), but also be used as important chemical raw materials such as iodine, mannitol, alginate (such as kelp, Gracilaria, As the raw material for extraction of mar...

Claims

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Application Information

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IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 姜鹏吴春辉张光涛陈华新李富超赵瑾
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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