Preparation method of extractive containing gastrodin and gastrodia elata polysaccharide

A technology of Gastrodia elata polysaccharides and extracts, which is applied in the field of extract preparation, can solve the problems of only Gastrodia elata polysaccharide content, cumbersome process, and high equipment requirements, and achieve the effects of broad market application prospects, short process flow, and good therapeutic effect

Active Publication Date: 2014-08-27
四川中医药高等专科学校 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN201210035854.4 discloses a new method for extracting Gastrodia elata polysaccharide and gastrodin at the same time. After the dry Gastrodia elata powder is extracted with water and alcohol-precipitated, the precipitate is washed with alcohol, dried under reduced pressure, and pulverized to obtain Gastrodia elata polysaccharide. Concentrate the alcohol filtrate under reduced pressure to obtain gastrodin extract; in the extract obtained by this method, the gastrodin content is only 2.6-2.8%, which is obtained by water extraction, alcohol precipitation, and concentration under reduced pressure to obtain gastrodin extract, wherein the polysaccharide The preparation method goes through the steps of suction filtration, which ta

Method used

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Effect test

Embodiment 1

[0030]Take the gastrodia elata medicinal material, crush the gastrodia elata medicinal material, pass through a No. 1 sieve, and take 100g. Put the medicinal materials that have passed the No. 1 sieve into the extraction kettle, reflux extraction for 3 times, combine the extracts to obtain the first extract, and use the remaining filter residue for later use. For each reflux extraction, pour 400 mL of 70% ethanol into the extraction kettle and reflux for 2 hours. Carry out vacuum distillation on the first extract, recover the ethanol therein until there is no ethanol smell, and obtain the extract. Add 155mL of water to the extract, mix well, and then add 130ml of D101 macroporous adsorption resin for adsorption to obtain D101 macroporous adsorption resin for absorbing Gastrodia elata extract, hereinafter referred to as the first resin. Take the chromatographic column, add 420mL of D101 macroporous adsorption resin that has not been adsorbed to the chromatographic column, afte...

Embodiment 2

[0033] After taking Gastrodia elata medicinal material and pulverizing it, pass through a No. 1 sieve, take 350g, and record it as the raw material. Using ethanol to reflux extract the raw material for 3 times, combine the filtrates to obtain the first extract, and collect the filter residue after reflux extraction for future use. Among them, 1400 mL of 70% ethanol was used for single reflux extraction, and the time for single reflux extraction was 2 hours. Carry out vacuum distillation on the first extract, recover the ethanol therein until there is no ethanol smell, and obtain the extract. Add 545mL of water to the extract, mix evenly, then add 460ml of D101 macroporous adsorption resin for adsorption, and after sufficient adsorption, D101 macroporous adsorption resin for absorbing Gastrodia elata extract is obtained.

[0034] Take another chromatographic column, add 1500mL of D101 macroporous adsorption resin that has not been adsorbed to the chromatographic column, and af...

Embodiment 3

[0039] Take Gastrodia elata medicinal material and crush it, pass through a No. 1 sieve, take 600g, and record it as raw material. Using ethanol to reflux extract the raw material for 3 times, combine the filtrates to obtain the first extract, and collect the filter residue after reflux extraction for future use. Among them, 2700 mL of 70% ethanol was used for single reflux extraction, and the time for single reflux extraction was 2 hours. Carry out vacuum distillation on the first extract, recover the ethanol therein until there is no ethanol smell, and obtain the extract. Add 930mL of water to the extract, mix well, then add 780ml of D101 macroporous adsorption resin for adsorption, after sufficient adsorption, obtain D101 macroporous adsorption resin for absorbing Gastrodia elata extract.

[0040] Take another chromatographic column, add 2500mL of D101 macroporous adsorption resin that has not been adsorbed to the chromatographic column, and after the D101 macroporous adso...

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Abstract

The invention discloses a preparation method of an extractive containing gastrodin and gastrodia elata polysaccharide. According to the method, gastrodin and gastrodia elata polysaccharide can be extracted from gastrodia elata as much as possible. The method comprises the following steps that firstly, alcohol extraction is adopted; secondly, reduced pressure distillation is performed on a first extracting solution, and adsorption is performed through D101 macroporous adsorption resin; thirdly, new D101 macroporous adsorption resin is added into a chromatographic column, the D101 macroporous adsorption resin with the gastrodia elata extracting solution adsorbed is placed above the new D101 macroporous adsorption resin in the chromatographic column to form a stereo adsorption and elution structure, and the gastrodia elata extracting solution is obtained; fourthly, filter residue is extracted through water at 75 DEG C-85 DEG C, and a gastrodia elata polysaccharide extracting solution, namely the extractive containing gastrodin and gastrodia elata polysaccharide, is obtained. The preparation method does not have high equipment requirements, equipment investment and cost are low, and therefore the preparation method is suitable for industrialized mass production. Besides, the preparation process can be effectively simplified, extraction cost is lowered, and the preparation method has significance for the extraction and application of gastrodia elata.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the field of gastrodia elata extraction, in particular to a preparation method of an extract containing gastrodin and gastrodia elata polysaccharide. Background technique [0002] Gastrodia elata is a perennial herbaceous plant of the genus Gastrodia elata in the family Orchidaceae ( Gastrodia elata Blume ) of dried tubers. It has the effects of relieving wind, relieving convulsions and convulsions, dispelling wind and eliminating numbness, and can be used for the treatment of hypertension, dizziness, headache, crooked mouth and eyes, various numbness of limbs, convulsions in children and other diseases. It has a long history. The main chemical components contained in Gastrodia tuber are gastrodin and its aglycone, vanillyl alcohol, other phenolic compounds, and sitosterol, carotin, citric acid, palmitic acid, succinic acid and trace elements, as well as a large number of polysaccharide...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P37/04A61P39/00A61P9/12A61P29/00A61P31/12A61P25/24A61P39/06A23L1/09C08B37/00A61K31/7034
Inventor 金虹江洪波李风李奇志王涛
Owner 四川中医药高等专科学校
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