Preparation method of extractive containing gastrodin and gastrodia elata polysaccharide

Abstract

The invention discloses a preparation method of an extractive containing gastrodin and gastrodia elata polysaccharide. According to the method, gastrodin and gastrodia elata polysaccharide can be extracted from gastrodia elata as much as possible. The method comprises the following steps that firstly, alcohol extraction is adopted; secondly, reduced pressure distillation is performed on a first extracting solution, and adsorption is performed through D101 macroporous adsorption resin; thirdly, new D101 macroporous adsorption resin is added into a chromatographic column, the D101 macroporous adsorption resin with the gastrodia elata extracting solution adsorbed is placed above the new D101 macroporous adsorption resin in the chromatographic column to form a stereo adsorption and elution structure, and the gastrodia elata extracting solution is obtained; fourthly, filter residue is extracted through water at 75 DEG C-85 DEG C, and a gastrodia elata polysaccharide extracting solution, namely the extractive containing gastrodin and gastrodia elata polysaccharide, is obtained. The preparation method does not have high equipment requirements, equipment investment and cost are low, and therefore the preparation method is suitable for industrialized mass production. Besides, the preparation process can be effectively simplified, extraction cost is lowered, and the preparation method has significance for the extraction and application of gastrodia elata.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the field of gastrodia elata extraction, in particular to a preparation method of an extract containing gastrodin and gastrodia elata polysaccharide. Background technique [0002] Gastrodia elata is a perennial herbaceous plant of the genus Gastrodia elata in the family Orchidaceae ( Gastrodia elata Blume ) of dried tubers. It has the effects of relieving wind, relieving convulsions and convulsions, dispelling wind and eliminating numbness, and can be used for the treatment of hypertension, dizziness, headache, crooked mouth and eyes, various numbness of limbs, convulsions in children and other diseases. It has a long history. The main chemical components contained in Gastrodia tuber are gastrodin and its aglycone, vanillyl alcohol, other phenolic compounds, and sitosterol, carotin, citric acid, palmitic acid, succinic acid and trace elements, as well as a large number of polysaccharide...

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Problems solved by technology

Chinese patent CN201210035854.4 discloses a new method for extracting Gastrodia elata polysaccharide and gastrodin at the same time. After the dry Gastrodia elata powder is extracted with water and alcohol-precipitated, the precipitate is washed with alcohol, dried under reduced pressure, and pulverized to obtain Gastrodia elata polysaccharide. Concentrate the alcohol filtrate under reduced pressure to obtain gastrodin extract; in the extract obtained by this method, the gastrodin content is only 2.6-2.8%, which is obtained by water extraction, alcohol precipitation, and concentration under reduced pressure to obtain gastrodin extract, wherein the polysaccharide The preparation method goes through the steps of suction filtration, which takes a long time, requires high equipment, and the process is cumbersome, which is not suitable for industrial continuous production

Chinese patent application CN201310585026 discloses a method for extracting Gastrodia elata polysaccharide, which is prepared by microwave and ultrafiltration membrane, the equipment cost is high, and the content of Gastrodia elata polysaccharide is only 55~62%

Another patent uses fresh Gastrodia elata to simultaneously extract gastrodin and Gastrodia elata polysaccharides. However, this method takes a long time to operate, the process is cumbersome, and the storage time of fresh Gastrodia elata is limited, which also limits the industrial application of this method and cannot meet the needs of actual production.