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Detection method for rapid identification of aspergillus fumigatus

A technology for detection of Aspergillus fumigatus, applied in the direction of microorganism-based methods, measurement/inspection of microorganisms, biochemical equipment and methods, etc., which can solve problems such as economic losses, pollution of food and feed products, and harm to human and animal health

Inactive Publication Date: 2014-09-17
YANGTZE UNIVERSITY
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Problems solved by technology

At the same time, the toxin produced by Aspergillus fumigatus seriously pollutes food and feed products, endangers the health of humans and animals, and causes major economic losses. Aspergillus fumigatus has become a hot spot for clinical medicine and medical microbiologists

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  • Detection method for rapid identification of aspergillus fumigatus

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with specific embodiments.

[0019] 1. Cultivation of Aspergillus strains to be tested

[0020] Aspergillus samples to be tested were obtained from the Institute of Microbiology, Chinese Academy of Sciences, the Fungi and Mycosis Research Center of Peking University, and the Fungal Research Center of Jilin University. They were inoculated onto PDA medium and cultured in an incubator at 28°C.

[0021] 2. DNA extraction of Aspergillus strains to be tested

[0022] Pick the hyphae pieces of Aspergillus strains with a diameter of 5mm and inoculate them into 100ml PD liquid medium, culture them on a shaker at room temperature (250rpm) for 2-4 weeks, collect the bacteria and rinse them with sterile saline several times, and blot them dry with sterile filter paper . Take 0.1 g of bacteria and quickly grind it into powder in liquid nitrogen, extract DNA with 2% (w / v) CTAB buffer, and detect DNA samples on 0....

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Abstract

The invention relates to a detection method for rapid identification of aspergillus fumigatus, and belongs to the technical field of molecular biological detection; the method is characterized by comprising the following steps: (1) extracting total DNA of a to-be-detected aspergillus fumigatus strain; (2) choosing RPB2 gene as a molecular marker, namely an objective fragment, and designing 3 specific primers; (3) amplifying the objective fragment of the to-be-detected aspergillus fumigatus strain by PCR (polymerase chain reaction); and (4) performing agarose gel electrophoresis detection, and judging whether the to-be-detected aspergillus fumigatus strain is aspergillus fumigates according to the electrophoresis detection results. The detection method can rapidly identify the aspergillus fumigatus, and has the advantages of being rapid, accurate and efficient.

Description

Technical field: [0001] The invention relates to a detection method for rapidly identifying Aspergillus fumigatus, belonging to the technical field of fungal molecular biology detection. Background technique: [0002] Fungi exist in almost all terrestrial and aquatic ecosystems, and are closely related to human production activities and life. Many fungi are of economic importance, but there are also species of fungi that cause diseases in plants, animals and humans or produce toxins that can endanger human health. [0003] Aspergillus fumigatus is a saprophytic fungus that widely exists in nature and is also an important opportunistic pathogen. As the immunocompromised population increases, 90% of systemic aspergillosis is caused by Aspergillus fumigatus. At the same time, the toxin produced by Aspergillus fumigatus seriously pollutes food and feed products, endangers the health of humans and animals, and causes significant economic losses. Aspergillus fumigatus has become...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/68
CPCC12Q1/686C12Q2565/125
Inventor 余知和曾昭清周建芬程云方
Owner YANGTZE UNIVERSITY
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