Method for extracting and purifying sulforaphane from broccoli seeds

A technology of broccoli seeds and sulforaphane, which is applied in organic chemistry and other fields, can solve the problems of insufficient extraction capacity and reagent pollution, and achieve the effects of high product yield, low production cost and simple process route

Inactive Publication Date: 2014-10-08
SUZHOU ZEDAXINGBANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem mainly solved by the present invention is to provide a method for extracting and purifying sulforaphane from broccoli seeds, so as to overcome the defects of insufficient extraction capacity and reagent pollution in existing small batches and laboratories, and meet the needs of large-scale industrial production , so as to lay the foundation for the development of sulforaphane into drugs and health products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 200 g of broccoli seeds, grind them through a pulverizer and pass through a 10-mesh medicine sieve. Put the sieved powder into a 5000 mL round bottom flask, then add 2000 mL of petroleum ether, and degrease at 60 °C for 5 h. After degreasing and filtering, the powder was put into a vacuum oven and dried at 70 °C for 3 h. Take out the powder and put it into a 5000 mL beaker, add 1000 mL of purified water to the beaker, adjust the pH value of the liquid in the beaker to 2.0 with pre-configured 2 mol / L hydrochloric acid, and hydrolyze at 40 °C for 5 h. After the hydrolysis, 2000 mL of purified water was added, the pH value of the liquid in the beaker was adjusted to 3.0 with pre-configured 2 mol / L hydrochloric acid, and the ultrasonic wave was extracted for 0.5 h at 20 KHz. After suction filtering the extracted mixed solution, pour the extract into a 10 L separatory funnel, add 600 mL of water-saturated ethyl acetate for extraction, and collect the organic phase in t...

Embodiment 2

[0029] Take 200 g of broccoli seeds, grind them through a pulverizer and pass through a 24-mesh medicine sieve. Put the sieved powder into a 5000 mL round bottom flask, then add 2000 mL of n-hexane, and degrease at 80 °C for 2 h. After degreasing and filtering, the powder was put into a vacuum oven and dried at 50°C for 3 h. Take out the powder and put it into a 5000 mL beaker, add 600 mL of purified water to the beaker, adjust the pH value of the liquid in the beaker to 4.0 with pre-configured 2 mol / L sulfuric acid, and hydrolyze at 50 °C for 3 h. After the hydrolysis, 4000 mL of purified water was added, the pH value of the liquid in the beaker was adjusted to 6.0 with a pre-configured 2 mol / L sulfuric acid, and the 10 KHz ultrasonic wave was extracted for 1 h. After suction filtering the extracted mixed solution, pour the extract into a 10 L separatory funnel, add 600 mL of water-saturated ethyl acetate for extraction, and collect the organic phase in the upper layer of th...

Embodiment 3

[0031] Take 200 g of broccoli seeds, grind them through a pulverizer and pass through a 50-mesh medicine sieve. Put the sieved powder into a 5000 mL round bottom flask, then add 2000 mL cyclohexane, and degrease at 70 °C for 3 h. After degreasing and filtering, the powder was put into a vacuum oven and dried at 60°C for 3 h. Take out the powder and add it to a 5000 mL beaker, add 800 mL of pre-configured citric acid-sodium hydroxide-hydrochloric acid buffer solution with a pH value of 3.0 to the beaker, and hydrolyze at 45 °C for 4 h. After hydrolysis, 3000 mL of pre-configured citric acid-sodium hydroxide-hydrochloric acid buffer solution with pH 3.0 was added, and 15 KHz ultrasonic wave was used for ultrasonic extraction for 0.8 h. After suction filtering the extracted mixed solution, pour the extract into a 10 L separatory funnel, add 600 mL of water-saturated ethyl acetate for extraction, and collect the organic phase in the upper layer of the solution. The extraction wa...

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Abstract

The invention discloses a method for extracting and purifying sulforaphane from broccoli seeds. The method comprises the following steps: 1, crushing broccoli seeds, degreasing the crushed broccoli seeds by using a low-polarity reagent in order to remove grease in the broccoli seeds, and drying the degreased broccoli seeds to obtain dry broccoli seed powder; 2, adding water and a pH value adjusting agent to the degreased and dried dry broccoli seed powder to obtain an acidic solution, and hydrolyzing the dry broccoli seed powder to obtain a hydrolysate; 3, adding water to the hydrolysate, carrying out ultrasonic extraction, and filtering the above obtained solution in order to obtain a sulforaphane extract liquid; and 4, adding a water saturated regent to the sulforaphane extract liquid to carry out extraction, collecting the obtained organic phase, and concentrating the organic phase to form an oily product in order to obtain oily sulforaphane. The method has the advantages of simple technology, low cost, good selectivity and the like, overcomes the preparation scale restriction of small batch and laboratories, and is in favor of the industrial production of sulforaphane.

Description

technical field [0001] The invention relates to a raw material processing method for both medicine and food, in particular to a method for extracting and purifying sulforaphane from broccoli seeds, which belongs to the field of deep processing of medicinal health products. Background technique [0002] Sulforaphane, also known as radionin, is a highly effective chemical functional substance and the strongest anti-cancer ingredient found in vegetables so far. Sulforaphane is an isothiocyanate (Isothiocyanates, referred to as ITCs) produced by enzymatic hydrolysis or acid hydrolysis of glucose glucosinolate (glucosinolate, a type of glucosinolate) by Myrosinase , named after the earliest discovery in radish, easily soluble in water, with a relative molecular weight of 177.3 and a molecular formula of C6H11S2NO. The structural formula of sulforaphane is shown in formula 1. [0003] [0004] Formula 1 Chemical structural formula of sulforaphane [0005] Sulforaphane is the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C331/20
Inventor 栾连军刘雪松谈满良李小冬李杰
Owner SUZHOU ZEDAXINGBANG MEDICAL TECH
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