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Phenolic acid type allelochemical degrading bacterium and preparation and application of microbial inoculum thereof

A technology for microbial inoculants and microbial strains, applied in the directions of biochemical equipment and methods, applications, and methods based on microorganisms, can solve problems such as damage to leaf photosynthetic mechanisms, photosynthetic rate, and stomatal conductance. Conducive to industrial production and the effect of short fermentation cycle

Active Publication Date: 2014-10-15
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, studies have shown that the self-toxic substances 2,6-di-tertiary phenol and dimethyl phthalate in eggplant reduce chlorophyll content, photosynthetic rate, and stomatal conductance, and damage the photosynthetic mechanism of leaves.

Method used

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  • Phenolic acid type allelochemical degrading bacterium and preparation and application of microbial inoculum thereof
  • Phenolic acid type allelochemical degrading bacterium and preparation and application of microbial inoculum thereof
  • Phenolic acid type allelochemical degrading bacterium and preparation and application of microbial inoculum thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Isolation of phenolic acid allelochemical-degrading bacteria BJS-1-3

[0030] Acclimatization of benzoic acid-degrading bacteria: Collect poplar rhizosphere soil that has been continuously cropped for many years, take 5g, add it to a conical flask containing 45ml of enrichment medium, and culture it in a shaker flask at 30°C and 150r / m. 3 days is a domestication cycle, a total of 4 After each cycle expired, the culture solution was transferred to the enrichment medium with an inoculum size of 10% (volume percentage) at 30°C and 150 r / m in shake flasks to continue culturing. The concentration of benzoic acid in the enrichment medium increased sequentially according to the acclimatization cycle, and the initial concentrations of benzoic acid in the four cycles were 500mg / L, 1000mg / L, 1500mg / L, and 2500mg / L, respectively.

[0031] Isolation of degrading bacteria with benzoic acid as the only carbon source: After the acclimation is completed in the enrichment medium cont...

Embodiment 2

[0049] Preparation of benzoic acid standard curve: prepare benzoic acid standard solution with a concentration of 1 mg / ml into a benzoic acid standard solution with a concentration of 0.1 mg / ml. Take benzoic acid standard solution 0.00ml, 1.00ml, 2.00ml, 4.00ml and 6.00ml respectively in a 100ml volumetric flask, add water to make up to 100ml, and shake well. Take blank as reference, measure its absorbance with quartz cuvette at 230nm, take absorbance as ordinate, and take the concentration of benzoic acid as abscissa to make standard curve ( figure 2 ).

[0050] Determination of benzoic acid degradation ability: use an inoculation loop to pick up the BJS-1-3 strains cultured on 2-ring plates, inoculate them into inorganic salt medium (MM) containing 1000mg / L benzoic acid, and place them on a shaker at 30°C and 200r / min Cultivate for 24 hours as seed solution. Then the seed solution was inoculated into MM medium containing 1000 mg / L benzoic acid with an inoculation amount o...

Embodiment 3

[0053] Use an inoculation loop to pick up the BJS-1-3 strain cultured on the 2-ring plate, inoculate it into the inorganic salt medium (MM) containing 1000 mg / L benzoic acid, and cultivate it on a shaker at 30°C and 200 r / min for 24 hours as the seed solution . The seed liquid is inoculated to six kinds of inorganic salts of ferulic acid, p-hydroxybenzoic acid, vanillin, salicylic acid, cinnamic acid, phloridin respectively with 2% (volume percentage) inoculation amount respectively again Cultivate in the culture medium on a shaker at 30°C and 200r / min, and observe whether the culture medium becomes turbid during 2d-7d. If it becomes turbid, it means that the above carbon source can be used; otherwise, it cannot. It was found that the BJS-1-3 strain could utilize the other five phenolic acids mentioned above except salicylic acid. It shows that the BJS-1-3 strain has substrate diversity and can use ferulic acid, p-hydroxybenzoic acid, vanillin, cinnamic acid and phlorizin as ...

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Abstract

The invention relates to a phenolic acid type allelochemical degrading bacterium and the preparation and the application of a microbial inoculum thereof. The BJS-1-3 bacterial strain disclosed by the invention is from rhizosphere soil of perennial poplars in Tai'an City, Shandong Province, China, and the soil is artificially gathered, screened and purified to obtain the bacterial strain. The bacterial strain has a high degrading capacity to benzoic acid, and phenolic acid type allelochemicals, such as ferulic acid, p-hydroxybenzoic acid, vanillin, cinnamic acid and phloridzin, can be respectively used as a unique carbon source in which the bacterial strain grows and propagates, and the bacterial strain can stably propagate in the rhizosphere of poplars, apple trees and cucumber plants. Through the adoption of the microbial inoculum prepared from the bacterial strain, the succession cropping obstacles of plants, such as the poplars, the apple trees and the cucumber plants, due to the accumulation of phenolic acid type allelochemicals can be effectively prevented and treated. The preparation technology of the microbial inoculum is simple, the fermentation period is short, the cost is low, and the industrialized production is facilitated.

Description

technical field [0001] The invention relates to the preparation and application of a strain of phenolic acid allelochemical-degrading bacteria and its agent, belonging to the technical field of agricultural microorganisms. Background technique [0002] Some plants can release some substances to inhibit the growth of the same species or the same family of plants in the same stubble or the next stubble through the ways of leaching from the ground, root secretion and plant residue decomposition. This phenomenon is called autotoxicity. Substances that produce autotoxicity include organic acids, straight-chain alcohols, simple phenols, phenolic acids, tannins, aldehydes, terpenes, amino acids, and alkaloids. Studies have shown that phenolic acid compounds such as benzoic acid, p-hydroxybenzoic acid, cinnamic acid, and phlorizin are the main autotoxic substances of plants such as poplar, cucumber, and apple. Under continuous cropping conditions, the accumulation of autotoxic subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01B79/00C12R1/085
Inventor 刘训理赵旭毛志泉隋君康姜红霞洒荣波王晓辉肖成泽王伟燕
Owner SHANDONG AGRICULTURAL UNIVERSITY
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