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Selective breeding and application of bacillus subtilis for alleviating stress of livestock and poultry

A technology of Bacillus subtilis and livestock and poultry stress, which is applied in the fields of application, bacteria, and animal husbandry. It can solve the problems of differences in the effect of probiotics and achieve the effects of improving production performance, relieving stress, and enhancing antibody titer

Active Publication Date: 2014-10-15
湖北华大瑞尔科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The crux of the problem is: the breeding of probiotics is mostly done through in vitro experiments, which is different from evaluating the effect of probiotics in complex real situations in animals

Method used

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  • Selective breeding and application of bacillus subtilis for alleviating stress of livestock and poultry
  • Selective breeding and application of bacillus subtilis for alleviating stress of livestock and poultry
  • Selective breeding and application of bacillus subtilis for alleviating stress of livestock and poultry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation and identification of strains

[0026] 1. Separation of strains

[0027] Collect 0.5 g of the rectal contents of chickens from a chicken farm in Hubei Province in 50 ml of sterile water, treat them in a water bath at 80°C for 15 minutes, select a suitable dilution factor to coat LB medium, culture at 37°C for 24 hours, and pick out typical spore colonies in LB Streak pure culture on the plate, and isolate a strain with round, white or slightly yellow colonies, irregular edges, opaque leaves, and obvious wrinkles in the middle of the colony. Observed by gram staining and microscopy, the gram-positive bacteria were long rod-shaped at 12h, arranged in single, pair or chain; at 24h, spores had been formed, and the spores were mesogenic or near mesophytic. The strain was named HDRaBS1 , Pure culture strains, all subcultured and preserved. See the growth characteristics of this strain on LB agar medium figure 1 , See Gram staining characteristics figure 2 -...

Embodiment 2

[0048] Example 2: Verification of antibacterial performance of strains

[0049] The fermentation supernatant of the strain HDRaBS1 of the present invention is prepared, and the bacteriostatic test is simultaneously performed on the common intestinal pathogenic bacteria Staphylococcus aureus, Escherichia coli and Salmonella. The test shows that the antibacterial effect of the strain HDRaBS1 of the present invention is the best among the selected strains. The results are shown in Figure 5 And table 2.

[0050] Table 2 Bacillus subtilis strain HDRaBS1 fermentation supernatant liquid in vitro antibacterial test

[0051]

[0052] Description of Table 2:: Inhibition zone diameter> 20mm is sensitive++; 20mm-15mm is more sensitive+; 0mm is insensitive.

Embodiment 3

[0053] Example 3: Heat stress feeding test of laying hens

[0054] The Bacillus subtilis strain HDRaBS1 is used as an inoculum to feed large-scale animal populations with obvious heat stress, and the purpose is to verify the anti-stress characteristics of the strain of the present invention at an in vivo level.

[0055] 1. Test grouping situation

[0056] In the animal experiment, 868 280-day-old commercial laying hens with similar egg production rates were selected and divided into 3 groups with 3 replicates in each group. The test period was 21 days. The basic diet was a conventional formula (W.Deng et al2012) , The specific feeding test grouping treatment is shown in Table 3:

[0057] Table 3 Feeding test grouping treatment

[0058]

[0059] 2. Test process

[0060] The feeding test time is the laying stage of laying hens, which starts at 280 days and ends at 300 days. The test period is 21 days. During the test period, the chickens were free to drink water, fed twice a day with the...

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Abstract

The invention belongs to the application field of preparation technologies of microorganism additives for livestock, and particularly relates to the selective breeding and the application of bacillus subtilis for alleviating the stress of livestock and poultry. The Bacillus subtilis (Bacillus subtilis) HDRaBS1 which has an obvious bacteriostasis effect on common intestinal tract pathogenic bacteria of breeding animals, such as staphylococcus aureus, escherichia coli and salmonella, is separated and screened from a poultry source, the bacterial strain is preserved in the China Center For Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M2011034. The verification of biological functions indicates that the bacterial strain has the characteristics of high stress resistance, stress resistance and the like, the bacterial strain can be used for preparing the microorganism preparation for resisting the stress of the livestock and the poultry, and the preparation comprises the microorganism additives for feed of the livestock and the poultry. The invention further discloses a separation and identification method of the bacillus subtilis HDRaBS1 and the application of the staphylococcus aureus for preparing single microbial inoculum for the livestock and the poultry.

Description

Technical field [0001] The invention belongs to the technical field of preparation of microbial additives for animals, and specifically relates to the selection and application of a Bacillus subtilis strain that relieves stress of livestock and poultry. The invention relates to the isolation and identification of a probiotic Bacillus subtilis strain and its preparation Application of feed additives to relieve stress of livestock and poultry. Background technique [0002] With the development of science and technology, antibiotics have been widely used in animal husbandry production with the functions of disease resistance and growth promotion, and the benefits brought to the breeding industry are beyond doubt. However, in recent years, the abuse of antibiotics has brought harm to the breeding industry and humans and has begun to highlight: such as causing animal immune function, endogenous animal infection and the production of a large number of drug-resistant strains, and the dr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16A23K1/18C12R1/125A23K10/18A23K50/75
Inventor 王喜亮张盼望肖运才周祖涛石德时晏涛毕丁仁况世昌吴贝金秀娥
Owner 湖北华大瑞尔科技有限公司
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