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A method for extracting rutin from Sophora japonica

A technology of Sophora japonica and rutin, applied in chemical instruments and methods, preparation of sugar derivatives, production of bulk chemicals, etc., can solve the problems of reduced production efficiency, rutin being dissolved in hot water, and high cost, and achieves easy operation, The effect of shortening extraction time and increasing yield

Active Publication Date: 2017-01-25
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods of deactivating enzymes adopted in the prior art mainly include dry steaming, ultraviolet irradiation, 60 Co irradiation, microwave enzyme inactivation, etc. Among them, the dry steaming temperature is high, which will cause the hydrolysis and oxidation of rutin while inactivating the enzyme, and will also cause part of the rutin to dissolve in hot water and be lost; while ultraviolet radiation can effectively inactivate the enzyme. , but it takes a long time and the cost is high, which reduces the production efficiency

Method used

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  • A method for extracting rutin from Sophora japonica
  • A method for extracting rutin from Sophora japonica
  • A method for extracting rutin from Sophora japonica

Examples

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Embodiment 1

[0016] Crush 20g of Sophora japonica to 30 mesh, add acid water with temperature of 35°C and pH=4~5, stir, put in supercritical CO 2 In the extraction device, treat at 30MPa, 30-40°C for 40min, then wash twice with acid water at 0-5°C, pH=4-5, and dry; add 4% borax to the dried Sophora japonica granules , 1% Na 2 SO 3 and 25 times of 60% ethanol, adding sodium bicarbonate to adjust the pH=9 to 10, ultrasonically extracting at 30kHz and 40°C in the dark for 30min, and then filtering, and the filtrate was kept in the dark at 30°C to 50°C for later use; Ultrasonic extraction was performed once under the same conditions, and the filtrates obtained from the two extractions were combined, 5% activated carbon was added to it, and stirred in the dark at 40°C for 10 minutes, the filtrate was acidified to pH = 4-5 after carbon removal, and 30kHz ultrasonic Treat in the dark at 0-5°C for 30 minutes, let stand to crystallize, and filter to obtain the crude rutin; place the crude rutin i...

Embodiment 2

[0018] Crush 30g of Sophora japonica to 50 mesh, add acid water with temperature of 40°C and pH=4~5, stir, put in supercritical CO 2 In the extraction device, treat at 30MPa, 30-40°C for 40min, then wash twice with acid water at 0-5°C, pH=4-5, and dry; add 3% borax to the dried Sophora japonica granules , 2% Na 2 SO 3 and 20 times of 60% ethanol, adjust the pH=9 to 10 with sodium bicarbonate, and extract it under the conditions of 25kHz and 35°C in the dark for 30 minutes, then filter, and the filtrate is kept in the dark at 30°C to 50°C for later use; Extract once under the same conditions as above, combine the filtrates obtained from the two extractions, add 5% activated carbon to it, and stir in the dark at 40°C for 15 minutes, acidify the filtrate after filtering and removing carbon to pH=4~5, and use a 20~30kHz Ultrasonic treatment at 0-5°C in the dark for 30 minutes, standing for crystallization, and suction filtration to obtain crude rutin; put the crude rutin in an o...

Embodiment 3

[0020] Crush 50g of Sophora japonica to 20 mesh, add acid water with temperature of 35°C and pH=4~5, stir, put in supercritical CO 2 In the extraction device, it was treated at 30MPa and 30-40°C for 40min, then washed twice with acid water at 5°C and pH=4-5, and then dried; add 4% borax, 1 %Na 2 SO 3 and 30 times of 70% ethanol, adjust the pH=9 to 10 with sodium bicarbonate, and extract it under the conditions of 30kHz and 40°C in the dark for 30 minutes, then filter, and the filtrate is kept in the dark at 30°C to 50°C for later use; Ultrasonic extraction once under the same conditions, combined the filtrates obtained from the two extractions, added 5% activated carbon to it, and stirred in the dark at 40°C for 15 minutes, acidified the filtrate after filtering and removing carbon to pH=4~5, and used 20~30kHz Ultrasonic treatment at 0-5°C in the dark for 30 minutes, static crystallization, and suction filtration to obtain crude rutin; put the crude rutin in an oven, dry at ...

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Abstract

The invention discloses a new method for extracting rutin from pagodatree flower bud. The method comprises the following steps: smashing the pagodatree flower bud, killing enzyme by adopting a supercritical CO2 method, carrying out ultrasonic-assisted extraction by using 60-70% ethyl alcohol under a certain condition to obtain an extracting solution, standing and crystallizing the extracting solution, carrying out suction filtration to obtain rutin crude products, refining the rutin crude products by using n-butanol, drying at the temperature of 130 DEG C to obtain rutin products with the purity being above 97% and the yield being above 22%. The method disclosed by the invention is simple and convenient to operate and high in extracting efficiency and is suitable for production practice.

Description

technical field [0001] The application relates to a method for extracting rutin from Sophora japonica. Background technique [0002] Sophora japonica is the dried flowers and flower buds of Sophora japonica, a leguminous plant. It has been used as medicine for more than 2,000 years. It is listed as top grade in "Shen Nong's Materia Medica". The rutin contained in Sophora japonica is about 8% to 28% (varies according to the place of origin and harvesting time of Sophora japonica). Rutin, also known as rutin and purple quercitrin, belongs to flavonoid glycosides. It has the effects of cooling blood and stopping bleeding, clearing liver and purging fire, reducing capillary permeability and fragility, promoting cell proliferation and preventing blood cell aggregation. Anti-inflammatory, anti-allergic, diuretic, antitussive, hypolipidemic and other effects. Rutin has been used clinically as an adjuvant treatment for hypertension, to prevent other bleeding disorders caused by ru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
CPCY02P20/54
Inventor 高瑞白易张成亮赵永强贾洪涛
Owner 内蒙古昶辉生物科技股份有限公司
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