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Selenium-enriched pleurotus eryngii cultivation and ingredient detecting methods

A technology for detecting king oyster mushrooms and components, which is applied in the fields of botanical equipment and methods, measuring devices, color/spectral characteristic measurement, etc. Benefit, increase yield, increase the effect of selenium enrichment capacity

Inactive Publication Date: 2014-11-12
ANHUI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current commercially available apricot apricot abalone is produced by traditional cutting and cultivation techniques. First, the selenium content is low, usually only 5 / zg / kg, which cannot be used as a dietary and health care product, and the added value is low; second, it cannot be produced all year round, only at 3 From June to October to December, the natural temperature is used to produce mushrooms, but in summer and winter, the production can only be stopped, and the market cannot be balanced all year round

Method used

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  • Selenium-enriched pleurotus eryngii cultivation and ingredient detecting methods
  • Selenium-enriched pleurotus eryngii cultivation and ingredient detecting methods
  • Selenium-enriched pleurotus eryngii cultivation and ingredient detecting methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the cutting and cultivating method of selenium-enriched Pleurotus eryngii

[0049] (1) Preparation medium: a, take the following raw materials by weight: 50 corncobs, 30 miscellaneous sawdust, 17 bran, 8 nutrient granules, 1 lime, 1 white sugar, 1 gypsum powder, 0.0001 sodium selenite; b 1. Crush the non-mildewed corncobs into the size of soybean grains and set aside; c. Mix sodium selenite and white sugar evenly, then add an appropriate amount of water and stir in a mixer for 1 minute to fully dissolve it, then add corncobs, Mix sawdust, bran, nutrient granules, lime, and gypsum powder for 5 minutes to make the compost fully absorb water; d. Spread the well-mixed medium on the cement floor for 24 hours, and turn it twice in the middle;

[0050] (2) Bagging: put the culture medium into a 17×33 cm polypropylene bag, each bag contains 1.5 kg of culture medium, compact it when filling the bag, put a collar on it, plug it with a silicone plug, and use a punche...

Embodiment 2

[0059] Embodiment 2: the selenium composition determination method of Pleurotus eryngii

[0060] (1) Determination of Selenium Content in Fruiting Body of Pleurotus eryngii

[0061] Mark the fully grown fruiting body and put it away as a sample. Wash the sample with distilled water for three times, dry it at 50°C, cool, pulverize, grind and weigh it. Because in this test, the quality of the obtained sample is less after drying, it is called The amount to be taken is also reduced as appropriate, accurately weigh 0.25 sample fine powder, add the volume ratio of HNO 3 : HClO 3 = 5ml of mixed acid solution composed of 2:1, soak and dissolve, bathe in a water bath at 100°C until the powder is completely dissolved, the mixed acid solution is clear, dilute the acid solution to 50ml, take 10ml of the diluted solution, put it into a conical flask, add 5 Mix %EDTA-2Na5ml, then adjust the pH to 2 with dilute nitric acid, add 3ml of 1% o-phenylenediamine solution, shake and shake for on...

Embodiment 3

[0066] Embodiment 3: the amino acid composition determination method of Pleurotus eryngii

[0067] (1) Pleurotus eryngii sample pretreatment

[0068] Clean the Pleurotus eryngii sample, dry it, separate the stipe and the cap, put the stipe and the cap respectively in a DHG-9240A electric heating constant temperature blast drying oven to dry to constant weight at 110°C, cool, and place in the laboratory Grind into fine powder in a bowl, pass through a 60-mesh sieve, and store in a desiccator for later use;

[0069] (2) Determination of amino acid content

[0070]Grind the above-mentioned different experimental materials, pass through 80 mesh sieve, accurately weigh 100mg of the analysis sample, put it into the hydrolysis tube, add 15-20ml of 6mol / L hydrochloric acid into the tube, vacuumize, and fill with 2000g / m 2 High-purity nitrogen, repeat this 3 times, seal the nozzle under nitrogen and place it at 110°C for 22 hours, take it out and cool it, filter the hydrolyzate, rins...

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Abstract

The invention discloses selenium-enriched pleurotus eryngii cultivation and ingredient detecting methods. The cultivation method comprises steps of culture medium preparation, bagging, sterilization, inoculation, fermentation, inducement to primordium, fruiting, harvesting and the like. Through control of main cultivation conditions of temperature, humidity, illumination, ventilation and the like, pleurotus eryngii is rich in selenium, can be produced all year round and comes into the market all year round, so that the yield is improved, the economic benefit is increased, the selenium enrichment capacity of pleurotus eryngii is improved, and the dietary therapy value of pleurotus eryngii is improved. The ingredient detecting method comprises detection of selenium elements, amino acid and total sugar, the amount of selenium of each kilogram of selenium-enriched pleurotus eryngii is 600 mu g / g, the selenium enrichment capacity is the best, and the amount of selenium doesn't exceed the limit absorption range, which is 800 mu g / d and is also the poisoning limit, of a human body.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for cultivation and component detection of selenium-enriched Pleurotus eryngii. Background technique [0002] Pleurotus eryngii, Chinese name: Pleurotus eryngii; commodity name: Pleurotus eryngii; Alias: Xueda, Xueying scallop swallow. Pleurotus eryngii has thick flesh, crisp and tender, delicious taste, with a light almond flavor, suitable for cooking various delicacies. The difference from other general varieties of oyster mushrooms, shiitake mushrooms, Coprinus comatus, etc. is that the organization is tight and elastic, and the storage time after picking is longer than that of ordinary mushrooms. Therefore, it is known as the "king of oyster mushrooms"; apricot abalone is rich in nutrition, high in protein, low in fat, and contains 18 kinds of amino acids, 8 of which are essential amino acids for the human body, and all of them are L-type, which are easy to be absorbed by the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00G01N21/31G01N5/00
Inventor 李能树何炎炘张志强
Owner ANHUI UNIVERSITY
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