Optical vacuum cold station for light microscope and electron microscope correlated imaging

A correlative imaging and vacuum cooling technology, which is applied in optics, optical components, and material analysis through optical means, can solve the problems of easy damage, easy pollution, and popularization of objective lens damage technology, so as to avoid the deformation of the carrier grid and solve the pollution problem effect

Active Publication Date: 2014-11-12
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing low-temperature cold stage design schemes use liquid nitrogen (or low-temperature nitrogen) flow refrigeration. The focal plane drift of the sample caused by liquid nitrogen vibration and the damage to the objective lens by ultra-l

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  • Optical vacuum cold station for light microscope and electron microscope correlated imaging
  • Optical vacuum cold station for light microscope and electron microscope correlated imaging

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0014] Example one:

[0015] Such as figure 1 As shown, this embodiment includes a vacuum chamber 1, one end of the vacuum chamber 1 is provided with an anti-pollution system adapter interface 2, and the other end is provided with an electron microscope sample rod adapter interface 3. An upper optical window 4 and a lower optical window 5 are provided on the upper and lower walls of the vacuum chamber 1 opposite to each other. The upper and lower optical windows 4 and 5 can be connected to the vacuum chamber 1 by bolts to facilitate disassembly. A vacuum system adapting interface 6 is provided on one side of the vacuum chamber 1, which is used to connect a vacuum pump group for extracting vacuum for the vacuum chamber 1. A vacuum valve 7 is provided in the vacuum chamber 1 between the electron microscope sample rod adapter interface 3 and the upper and lower optical windows 4, 5. A pre-vacuum is formed between the vacuum valve 7 and the electron microscope sample rod adapter inte...

Example Embodiment

[0023] Embodiment two:

[0024] Such as figure 1 As shown, on the basis of the first embodiment, this embodiment also includes an anti-pollution system. The anti-pollution system includes a heat conduction rod 9 partially extending into the vacuum chamber 1 through the anti-pollution system adapting interface 2. The heat conduction rod 9 is located One end of the vacuum chamber 1 is connected to a metal cold box 10, the metal cold box 10 is opposite to the upper and lower optical windows 4 and 5, and a pair of light-passing holes are provided on the upper and lower surfaces of the metal cold box 10 (in the figure) Not shown). One end of the heat conducting rod 9 located outside the vacuum chamber 1 is connected to a heat conducting core 11. The anti-pollution system also includes a Dewar tank 12. A liquid nitrogen chamber is formed inside the Dewar tank 12. The liquid nitrogen chamber is filled with liquid nitrogen and the tank opening is covered by the liquid nitrogen chamber c...

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Abstract

The invention relates to an optical vacuum cold station for light microscope and electron microscope correlated imaging. The optical vacuum cold station comprises a vacuum chamber, an anti-pollution system adapter interface, an electron microscope sample lever adapter interface, an upper optical window, a lower optical window, a vacuum system adapter interface and a vacuum valve, wherein the anti-pollution system adapter interface is arranged in one end of the vacuum chamber, the electron microscope sample lever adapter interface is arranged in the other end of the vacuum chamber, the upper optical window is arranged in the upper wall of the vacuum chamber, the lower optical window is arranged in the lower wall of the vacuum chamber and opposite to the upper optical window, the vacuum system adapter interface is arranged in one side of the vacuum chamber, and the vacuum valve is arranged between the electron microscope sample lever adapter interface and the combination of the upper optical window and the lower optical window; a forvacuum channel is formed between the vacuum valve and the electron microscope sample lever adapter interface, and a forvacuum valve is arranged on one side of the forvacuum channel; vacuum seal rings are respectively arranged on the electron microscope sample lever adapter interface, the upper optical window, the lower optical window, the vacuum system adapter interface and the anti-pollution system adapter interface. The optical vacuum cold station can be widely applied to the light and electron correlated imaging and can also be applied to optical microscopic imaging independently.

Description

technical field [0001] The invention belongs to the technical field of optical imaging, and in particular relates to an optical vacuum cooling table for associated imaging with an optical microscope and an electron microscope. Background technique [0002] The three-dimensional reconstruction technology of cryo-electron microscopy, X-ray crystallography, and nuclear magnetic resonance spectroscopy have become the most important experimental methods for high-resolution structural biology research, and more and more structures of biological macromolecules have been resolved one after another. However, people's ultimate goal is to analyze the structure of biological macromolecules and molecular machines in situ, so as to explain the essence of life activities at different levels such as molecules, organelles, and cells. [0003] In recent years, through the integration of fluorescent protein labeling technology, fluorescence microscopy, immuno-electron microscopy and cryo-elect...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N23/04G02B21/00H01J37/26
CPCG02B21/26G02B21/28H01J37/18H01J37/20H01J37/226H01J37/228H01J37/26H01J2237/182H01J2237/2001H01J2237/2002
Inventor 季刚李硕果孙飞
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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