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L-phenylalanine production process

A technology for phenylalanine and production process, which is applied in the field of separation technology, can solve the problems of high cost, low yield, serious environmental pollution and the like, and achieves the effects of reducing dosage, high elution rate and low consumption of ammonia water

Inactive Publication Date: 2014-12-24
山东信乐生物工程有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Due to the shortcomings of low yield, high cost, and serious environmental pollution, the first three methods have not been further industrialized and are now basically eliminated.

Method used

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  • L-phenylalanine production process

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with accompanying drawing.

[0026] The production process of L-phenylalanine, as shown in the figure, comprises the following steps: 1. Kezi cultivation. Put 25mL of L-phenylalanine medium in a 250mL Erlenmeyer flask, inoculate the slant of the strain, and incubate at 37°C for 12h on a rotary shaker. After the seed tank has been boiled for 1 hour, then sterilized by air and sterilized, it is inserted into the medium for submerged cultivation of the seeds. 2. Fermentation. Using advanced concentrated sugar feeding process, the sterilized medium and strains are connected to a new type of aerobic automatic control fermenter, and the ingredients are fully automatically controlled. Under the condition of ventilation and stirring, the fermenter is continuously fed and sterilized The concentrated sugar solution of bacteria is used to ensure the smooth progress of fermentation and obtain a higher acid produ...

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Abstract

The invention relates to an L-phenylalanine production process, which comprises the following steps of: 1, seed culture, wherein the first stage is shake-flask culture, and the second stage is seed-tank culture; 2, fermentation, wherein by using an advanced concentrated sugar feeding process, culture mediums and strains are inoculated to a novel aerobic automatic control fermentation tank to ferment for 50-55 hours at a temperature of 37 DEG C; 3, ceramic membrane and ultra-membrane filtration, wherein after the fermentation is completed, fermenting mash is acidified, by using a modern membrane separation technology and a cross-flow filtration technology, microfiltration and ultrafiltration organic membrane two-step filtration is established for removing the strains and most of proteins, so that produced liquid with a relatively high purity is obtained, and after the produced liquid is subjected to absorption-desorption by using an ion exchange column, the obtained product is concentrated and crystallized; 4, concentration, crystallization and separation, wherein after concentrated liquor is fed into a multi-effect evaporator for being concentrated and crystallized, the obtained product is fed into a crystallizing tank for evaporative crystallization, and the obtained object is separated for recovering phenylalanine; and 5, drying and packing. The process disclosed by the invention is advanced in technology and high in product recovery rate, saves manpower and reduces the energy consumption.

Description

technical field [0001] The invention relates to a production process of L-phenylalanine, in particular, it utilizes modern membrane separation technology and cross-flow filtration technology to establish an advanced process and separation process for extracting L-phenylalanine through two-step filtration of organic membranes of microfiltration and ultrafiltration. Background technique [0002] L-phenylalanine is an important amino acid with biological activity. Humans and animals cannot synthesize it by themselves and must ingest it from the outside. It is one of the eight essential amino acids and is also an important raw material for the synthesis of tyrosine in organisms. The most important use of L-phenylalanine is to synthesize the dipeptide product aspartame with L-aspartic acid, and it is also the raw material for the synthesis of various drugs. ingredients and feed additives. [0003] The industrialized production methods of L-phenylalanine mainly include natural pr...

Claims

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Application Information

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IPC IPC(8): C12P13/22C07C229/36C07C227/40
Inventor 王瑞平
Owner 山东信乐生物工程有限公司
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