Method for identification of functional components of arborvitae, fleece-flower root and mulberry leaves in hair-raising cosmetics
A technology of functional ingredients, Polygonum multiflorum, applied in the field of cosmetic analysis, can solve problems such as affecting consumers' confidence in hair-growing cosmetics, complex matrices of hair-growing cosmetics, and low content of three functional ingredients, achieving low detection limit, high result accuracy, and purification. good effect
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[0038] 1. Experimental instruments, materials and reagents
[0039] Main instruments: ultra-high performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometer (Waters, USA);
[0040] High-speed refrigerated centrifuge (Allegra64R, Beckman);
[0041] MS3basic vortex mixer (Germany IKA company);
[0042] BG-02C ultrasonic generator (Guangzhou Bangjie Ultrasonic Equipment Co., Ltd.);
[0043] 24-hole solid-phase extraction device (Shanghai Anpu Co., Ltd.);
[0044] Ultrapure water system (Milli-Q, American Millipore Company);
[0045] PVDF filter membrane (diameter 25m, pore size 0.2μm, American Agilent Company);
[0046] HLB solid-phase extraction column (Waters, USA, 200mg / 6mL);
[0047] PAX solid-phase extraction column (Agilent, USA, 500mg / 6mL).
[0048] Main reagents: methanol, acetonitrile (HPLC grade, Fisher Scientific); formic acid, ammonia water, disodium hydrogen phosphate (analytical grade, Shanghai Anpu Company);
[0049]Polygonidoside (T...
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