Method for identification of functional components of arborvitae, fleece-flower root and mulberry leaves in hair-raising cosmetics

Abstract

The invention discloses a method for identifying function components of platycladus orientalis, radix polygoni multiflori and folium mori in cosmetic of hair tonic. The method comprises the steps as follows: a sample is taken and dissolved by methyl alcohol, and 1.8%-2.2% of a methyl alcohol solution is added to obtain a liquid A; a PAX solid-phase extraction column is taken, activated and balanced by methyl alcohol and water, sampled, leached by 4.5%-5.5% of an ammonia-water solution and methyl alcohol and eluted by 1.8%-2.2% of a methanoic acid and methanol solution, an eluent is collected, dried, redissolved and filtered, filtrate is measured by ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry, wherein the chromatographic column is CSH C18, the flowing phase A is 0.0015%-0.0025% of an aqueous methanoic acid solution, and B is methyl alcohol; and gradient elution is performed. With the adoption of the method, the function components such as quercitrin, radix polygoni multiflori glycoside and rutin of latycladus orientalis, radix polygoni multiflori and folium mori in the cosmetic of hair tonic can be detected qualitatively and quantitatively, and the result is accurate and reliable.

Description

technical field [0001] The invention relates to the field of cosmetic analysis, in particular to a method for identifying functional components of arborvitae, Polygonum multiflorum and mulberry leaves in hair growth cosmetics. Background technique [0002] Hair loss is a common clinical disease. Although it is not a serious disease, it hinders the appearance and affects the appearance, and often brings huge mental pressure and psychological burden to the patient. As people pay more and more attention to hair loss, the market demand for hair growth cosmetics has increased sharply, and various new hair growth cosmetic products have emerged in an endless stream. According to inquiries, there are currently nearly 450 kinds of domestic cosmetics registered in my country's State Food and Drug Administration for the special certificate of hair growth cosmetics, most of which use Chinese herbal plant extracts as the main functional ingredients. [0003] Arborvitae, Polygonum multif...

AI Technical Summary

Problems solved by technology

However, although there are many research reports on the detection technology of quercitrin QR (formula Ⅰ), Polygonidoside THS-GLU (formula Ⅱ) and rutin RT (formula Ⅲ), the main methods are high-performance liquid chromatography and liquid chromatography. Chromatography and mass spectrometry, etc., but due to the low content of three functional components in hair growth cosmetics, and the complex matrix of hair growth cosmetics, especially hair growth shampoo products contain a large amount of various surfactants and other matrices, so now Some detection methods are not suitable for the identification of quercetin, polygogoside and rutin, the functional ingredients of arborvitae, Polygonum multiflorum and mulberry leaves in hair growth cosmetics; and due to the lack of relevant efficacy detection methods and identification technologies, counterfeit and shoddy, false publicity, etc. The phenomenon is flooding the entire hair growth cosmetics industry, seriously affecting consumers' confidence in Chinese herbal plant hair growth cosmetics

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