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Environmental protection technology for processing threonine fermentation wastewater by using biological agent

A technology for fermentation wastewater and biological preparations, which is applied in the technical field of threonine production in the biological fermentation industry to achieve the effects of fast reproduction, improved biological treatment efficiency and good degradation effect.

Active Publication Date: 2015-01-14
SHANDONG CHENZHANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no mature complete set of technology applied to production practice at home and abroad.
The main problem is that the one-time investment is too large, or the daily operation cost is too high, most manufacturers cannot bear it, and have to maintain the status quo of excessive emissions for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An environmental protection process for threonine fermentation wastewater, comprising the following steps:

[0033] (1) Centrifugal separation: use a high-speed disc separator to separate the bacterial protein in the threonine fermentation broth. The speed of the high-speed disc separator to separate the bacterial cells is 4000-5000 r / min, and the bacterial protein precipitate is recovered and collected. supernatant.

[0034] (2) Membrane filtration for secondary removal of bacterial protein: the supernatant obtained in step 1) is filtered with a ceramic membrane, the membrane pore size is 40 nm, wherein the filtration temperature is 37 ° C, the pressure into the membrane is 2.0 bar, and the residual bacteria are recovered by filtration. protein, and the filtrate was collected.

[0035] (3), thalline protein treatment: combine the thalli protein of step 1) and step 2), add an appropriate amount of warm water and mix thoroughly, adjust the solid content to 12-15%, put...

Embodiment 2

[0052] Take the waste water from the threonine extraction process of the threonine fermentation workshop in Fufeng, enter the sewage treatment system according to the method of Example 1, and add 20 grams of microbial preparations per cubic meter of bottom material each time, once a day, and continuously add For a week, let it stand for 3 days at the end, and discharge the liquid. The discharge standard is that the waste water should meet the first-level standard of the "Comprehensive Wastewater Discharge Standard" (GB8978-96), as shown in Table 1:

[0053] Table 1

[0054] Test items initial value Emission Standards After processing COD CR / mg / L 9700 100 73 BOD 5 / mg / L 2371 30 25 SS / mg / L 1768 70 37

[0055] After half a year of commissioning and operation, the sewage treatment system is operating reliably, the effluent quality is stable, and the treatment effect is good. In February 2014, the local env...

Embodiment 3

[0057] Effect test of probiotics prepared in Example 1:

[0058] The pig used in the experiment is Duroc × Junmu No. 1 hybrid pig

[0059] The control group drank tap water; the experimental group drank 1% aqueous solution of probiotics (weight ratio), with an average initial weight of 25.3kg,

[0060] The experimental group and the control group consisted of 20 pigs each, which were randomly divided into groups and reared under the same conditions for 98 days. The growth conditions are shown in Table 1:

[0061] Table 1: Comparative test data of probiotics on weight gain in pigs

[0062] group initial weight 33d weight 66d weight 98d weight Weight gain % control group 25.3 49.2 72.7 94.9 test group 25.3 53.5 82.1 105.4 11．1

[0063] The data in Table 1 shows that the growth ability of pigs in the experimental group is significantly higher than that in the control group, and the occurrence of gastrointestinal diseases such as dia...

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Abstract

The invention relates to an environmental protection technology for processing a threonine fermentation wastewater by using a biological agent. The process comprises the following steps: separating mycoprotein from threonine fermented liquid by virtue of a high-speed disc separator, recovering mycoprotein sediments, and collecting supernate; filtering the supernate by virtue of a film; recovering mycoprotein by virtue of the film, collecting filtrate, merging the mycoprotein, and adjusting the solid content to prepare a probiotic agent in a fermentation manner; concentrating, carrying out isopotential sedimentation on a production wastewater generated in the threonine extraction step; carrying out natural sedimentation and solid-liquid separation, discharging the supernate into a sewage treatment system; and adjusting the pH and adding a composite microbial agent to carry out advanced treatment, so as to discharge with standards. The process disclosed by the invention is low in wastewater discharge, economical and environmentally friendly, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of threonine production technology in the biological fermentation industry, and specifically provides an environmentally friendly process for treating threonine fermentation wastewater by using biological preparations Background technique [0002] Threonine (Threonine, abbreviated as Thr), scientific name 2-amino-3-hydroxybutyric acid, belongs to aliphatic amino acid, slightly sweet, named for its structure similar to threose, is a kind of human, animal and plant protein Essential amino acids are mainly used in medicine, chemical reagents, and nutritional enhancers. They can strengthen dairy products, restore human fatigue, and promote growth and development. In recent years, with the development of the economy, the market demand for threonine has continued to grow steadily, and it is one of the amino acid varieties with the fastest growing demand. Especially, the use in chemistry and biochemistry, food additives, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/14C02F3/34C02F103/36
CPCC02F1/38C02F1/44C02F3/341C02F9/00C02F2103/36
Inventor 卢松张传森王均成时夫龙曹光华李亚静
Owner SHANDONG CHENZHANG BIOTECHNOLOGY CO LTD
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