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Preparation method of elastic porous chitosan sponge

A technology of porous chitosan and chitosan, which is applied in the field of preparation of porous chitosan sponge, can solve the problems of narrow application and high price of cross-linking agent genipin, so as to avoid collapse, ensure biological safety, and wide The effect of applying the foreground

Inactive Publication Date: 2015-01-21
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cross-linking agent genipin used in this method is expensive, and the obtained sample has the function of shape recovery only under hydration, and its application is narrow.
So far, there is no report about elastic porous chitosan sponge

Method used

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  • Preparation method of elastic porous chitosan sponge
  • Preparation method of elastic porous chitosan sponge
  • Preparation method of elastic porous chitosan sponge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of elastic porous chitosan sponge

[0022] Weigh 1.0 g of chitosan powder, wherein the degree of deacetylation of chitosan is 98%, and the viscosity is 100 cp·s. Chitosan was dissolved in 50 mL of 1% (v / v) acetic acid to prepare a 2.0% (w / v) chitosan solution. Then pour it into a twelve-well culture plate and pre-freeze it in a -20°C refrigerator. Place the frozen chitosan solution in a vacuum freeze dryer for 24 hours to freeze-dry, wherein the parameters of the freeze dryer are: cold trap temperature<-52°C, vacuum degree<10Pa.

[0023] Use a pipette gun to pipette 700 μL of dodecanal, dissolve it in 30 mL of absolute ethanol, and sonicate until the dodecanal is completely dissolved to obtain a 0.2 mol / L clear and transparent cross-linking agent solution. The blank chitosan sponge was taken out from the 12-well culture plate, and soaked in the cross-linking agent solution, the soaking temperature was 70°C, and the soaking time was 8 hour...

Embodiment 2

[0025] Embodiment 2: the preparation of elastic porous chitosan sponge

[0026] Weigh 0.5 g of chitosan, wherein the degree of deacetylation of chitosan is 98%, and the viscosity is 200 cp·s. Chitosan was dissolved in 50 mL of 1% (v / v) acetic acid to prepare a 1.0% (w / v) chitosan solution. Then pour it into a twelve-well culture plate and pre-freeze it in a -20°C refrigerator. Place the frozen chitosan solution in a vacuum freeze dryer for 18 hours to freeze-dry, wherein the parameters of the freeze dryer are: cold trap temperature<-52°C, vacuum degree<10Pa.

[0027] Use a pipette gun to pipette 400 μL of tetradecaldehyde, dissolve it in 30 mL of absolute ethanol, and sonicate until the tetradecaldehyde is completely dissolved to obtain a clear and transparent cross-linking agent solution of 0.07 mol / L. Take the blank chitosan sponge out of the 12-well culture plate, put it into the cross-linking agent solution and soak it, the soaking temperature is 75°C, and the soaking ti...

Embodiment 3

[0028] Embodiment 3: the preparation of porous chitosan sponge with elasticity

[0029]Weigh 1 g of chitosan, wherein the degree of deacetylation of the chitosan is 70%, and the viscosity is 150 cp·s. Chitosan was dissolved in 50 mL of 1% (v / v) acetic acid to prepare a 2.0% (w / v) chitosan solution. Then pour it into a twelve-well culture plate and pre-freeze it in a -18°C refrigerator. Place the frozen chitosan solution in a vacuum freeze dryer for 20 hours to freeze-dry, wherein the parameters of the freeze dryer are: cold trap temperature<-52°C, vacuum degree<10Pa.

[0030] Use a pipette gun to pipette 300 μL of octadecaldehyde, dissolve it in 30 mL of absolute ethanol, and sonicate until octadecaldehyde is completely dissolved to obtain a 0.04 mol / L clear and transparent cross-linking agent solution. The blank chitosan sponge was taken out from the 12-well culture plate, and soaked in the cross-linking agent solution, the soaking temperature was 60° C., and the soaking ti...

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Abstract

The invention relates to a preparation method of elastic porous chitosan sponge. The preparation method comprises the following steps: dissolving fatty aldehyde in absolute ethyl alcohol to prepare a crosslinking agent solution, then soaking blank chitosan sponge into the crosslinking agent solution, taking out the chitosan sponge after a crosslinking reaction, repeatedly washing the chitosan sponge for multiple times by using absolute ethyl alcohol to clear away the crosslinking agent residual on the surface, and air drying the chitosan sponge at room temperature to obtain the elastic porous chitosan sponge. The method provided by the invention has the following beneficial effects: avoiding the defects of collapse and reduced volume of the chitosan sponge after being soaked in an alkali, avoiding the generation of a large amount of wastewater pollution, and ensuring the biosecurity of the sponge in human body; the porous chitosan sponge prepared from the method provided by the invention has elasticity, and the porous chitosan sponge has a broad application prospect in the field of porous scaffolds of tissue engineering due to this property.

Description

technical field [0001] The invention relates to a preparation method of an elastic porous chitosan sponge, which belongs to the technical field of preparation of biomedical materials and tissue engineering materials. Background technique [0002] Chitosan (chitosan) is obtained by deacetylation of chitin (chitin), which exists widely in nature, and its chemical name is polyglucosamine (1-4)-2-amino-B-D glucose. Since the Frenchman Rouget first obtained chitosan in 1859, the excellent properties of this natural polymer material, such as biofunctionality, compatibility, blood compatibility, safety, and biodegradability, have attracted widespread attention from all walks of life. Significant progress has been made in applied research in many fields such as medicine, food, chemical industry, cosmetics, water treatment, metal extraction and recovery, biochemical and biomedical engineering, especially in the field of tissue engineering porous scaffold materials. An ideal scaffold...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/20A61L27/00
Inventor 陈嵘高婧婷杨浩吕中
Owner WUHAN INSTITUTE OF TECHNOLOGY
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