Lactobacillus acidophilus capable of reducing drug resistance, and biological preparation prepared therefrom

A technology of Lactobacillus acidophilus and biological preparations, which is applied in the field of Lactobacillus acidophilus, can solve the problem of being unable to reduce the resistance of bacteria to antibiotics, and achieve the effect of reducing the difficulty of treatment and reducing drug resistance

Active Publication Date: 2015-01-21
HESHAN SEWETE BIOLOGICAL PROD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, the research and application of compound microbial bacterial agents have been very common. These microbial bacterial agents are formed by compound culture of multiple strains of a certain genus, and some are composed of multiple strains of one or more genera. Lactic acid bacteria, yeast, bacillus, bifidobacterium, etc. are the most widely used at present. These complex microbial agents with different formulas have been widely used in animal husbandry, aquaculture, and human health care. In the field, the most widely used y

Method used

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  • Lactobacillus acidophilus capable of reducing drug resistance, and biological preparation prepared therefrom
  • Lactobacillus acidophilus capable of reducing drug resistance, and biological preparation prepared therefrom

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example one:

[0028] Domestication and screening of Lactobacillus acidophilus XD13R strain: 4 strains of Bacillus (XD05B-XD08B), 3 strains of lactic acid bacteria (XD05R-XD07R) and 1 strain of yeast (XD05J) were purchased from the Culture Collection Center of Guangdong Institute of Microbiology. Guangzhou Baijia Supermarket purchased 2 strains of yeast (XD06J-XD07J), and then isolated and screened 8 strains of lactic acid bacteria (XD08-RXD15R) whose plaque and cell morphology were different from the above strains from the natural world. A total of 4 strains were collected. Bacillus (XD05B-XD08B), 11 strains of lactic acid bacteria (XD05R-XD15R), 3 strains of yeasts (XD05J-XD07J), and then each of them were subjected to mutagenesis in the water environment of pig manure with 10 generations of highly resistant bacteria, and finally selected Produce strains that can adapt to the above-mentioned pig manure water environment and can reduce the proportion of drug-resistant ba...

Example Embodiment

[0031] Embodiment two:

[0032] (1) Preparation of Lactobacillus acidophilus XD13R bacterial solution: inoculate a fresh culture of Lactobacillus acidophilus XD13R into 10-20 500ml Erlenmeyer flasks containing MRS medium, and cultivate in an incubator at 30°C 48h, get Lactobacillus acidophilus XD13R strain;

[0033] (2) Production of culture medium: use sucrose, peptone, yeast extract, KH 2 PO 4 , Tomato juice and water are the main raw materials to prepare the culture medium, and their weight percentages are:

[0034] Sucrose 4%, peptone 2.5%, yeast extract 1%, KH 2 PO 4 1%, tomato juice 4%, water balance.

[0035] (3) Inoculate the above-mentioned Lactobacillus acidophilus XD13R bacterial liquid into the above-mentioned medium that has been sterilized by high temperature, adjust the initial pH to 5~6.5, and carry out anaerobic fermentation in the fermentor for 15 days until the end pH is 3~ 4. The biological preparation prepared by Lactobacillus acidophilus XD13R is obtained.

Example Embodiment

[0036] Embodiment three:

[0037] (1) Preparation of Lactobacillus acidophilus XD13R broth: inoculate a fresh culture of Lactobacillus acidophilus XD13R into 10-20 500ml Erlenmeyer flasks containing MRS medium, and cultivate in an incubator at 38°C 40h, get Lactobacillus acidophilus XD13R bacterial liquid;

[0038] (2) Production of culture medium: use sucrose, peptone, yeast extract, KH 2 PO 4 , Tomato juice and water are the main raw materials to prepare the culture medium, and their weight percentages are:

[0039] Sucrose 4%, peptone 2.5%, yeast extract 1%, KH 2 PO 4 1%, tomato juice 4%, water balance.

[0040] (3) Inoculate the above-mentioned Lactobacillus acidophilus XD13R bacterial liquid into the above-mentioned medium which has been sterilized by high temperature, adjust the initial pH to 5~6.5, and carry out anaerobic fermentation in the fermentor for 4 days until the end pH is 3~ 4. The biological preparation prepared by Lactobacillus acidophilus XD13R is obtained.

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Abstract

The invention discloses a Lactobacillus acidophilus capable of reducing the drug resistance, and a biological preparation prepared therefrom. The Lactobacillus acidophilus disclosed in the invention is a new Lactobacillus acidophilus XD13R, has a preservation number of CCTCCM2014216, can reduce the proportion of drug resistant bacteria in animals, and can reduce the resistance of animals and human bodies to various antibiotics. The invention also discloses the biological preparation prepared by using the Lactobacillus acidophilus XD13R, and a preparation method of the biological preparation. The biological preparation is used to feed domestic animals in order to reduce the proportion of bacteria resisting different antibiotics in the animals by 30-96%.

Description

technical field [0001] The invention relates to a strain of Lactobacillus acidophilus capable of reducing drug resistance in animals, in particular to a strain of Lactobacillus acidophilus capable of reducing the proportion of drug-resistant bacteria in animals. The invention also relates to a biological preparation and a method prepared by using the new strain of Lactobacillus acidophilus. Background technique [0002] In the past 20 years, many diseases, such as tuberculosis and pneumonia, which have almost disappeared or are generally considered not to pose a major threat to human health, with the outbreak of infectious diseases and the growth of drug-resistant strains and their global re-spread, Once cast a pale shadow on the health of all mankind. In fact, we are entering the so-called "post-antibiotic era" - the dark age of uncontrollable infectious diseases before antibiotics were discovered, and today, several species of bacteria are resistant to almost all antibiot...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/23
CPCC12N1/20C12N1/205C12R2001/23
Inventor 杨雄
Owner HESHAN SEWETE BIOLOGICAL PROD
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