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Preparation method of antimicrobial coating on substrate surface

A substrate surface, antibacterial coating technology, applied in metal material coating process, coating, etc., can solve the problem of bacterial drug resistance enhancement, achieve good inhibition effect, overcome microbial drug resistance, and apply to substrates wide range of effects

Active Publication Date: 2015-01-28
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since this method is a biofilm control method based on a non-bactericidal mechanism, it overcomes the problem of enhanced bacterial resistance caused by traditional antimicrobial agents

Method used

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  • Preparation method of antimicrobial coating on substrate surface
  • Preparation method of antimicrobial coating on substrate surface
  • Preparation method of antimicrobial coating on substrate surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) The titanium plate is first surface polished, then ultrasonically cleaned with distilled water, acetone and absolute ethanol in turn, and finally vacuum-dried;

[0027] (2) Immerse the pretreated titanium plate in a mixed solution containing 1% Nafion and 10mg / L BBF, take it out after 12h, and store it after air drying;

[0028] (3) figure 1 It is the scanning electron micrograph of the antibacterial coating prepared in the present embodiment. The antibacterial performance experiment was carried out to the antibacterial coating prepared in this embodiment, and the mixed bacteria suspension prepared from the activated sludge of the sewage treatment plant was used as the experimental object. The inhibition rate was 61%.

Embodiment 2

[0030] (1) The titanium plate is first surface polished, then ultrasonically cleaned with distilled water, acetone and absolute ethanol in turn, and finally vacuum-dried;

[0031] (2) Dip the pretreated titanium plate in a mixed solution containing 0.5% Nafion and 10 mg / L BBF, take it out after 12 hours, and store it after air drying;

[0032] (3) Antibacterial performance experiment is carried out to the antibacterial coating prepared by the present embodiment, the mixed bacteria suspension prepared from the activated sludge of the sewage treatment plant is taken as the experimental object, and the results show that after the biofilm is cultivated for one day, the antibacterial coating has no effect on the mixed bacterial suspension. The inhibition rate of bacterial biofilm was 49%.

Embodiment 3

[0034] (1) The glass slide is first cleaned with detergent, rinsed with deionized water, rinsed with methanol, and then dried with nitrogen gas, and then silanized with aminopropyltriethoxysilane;

[0035](2) Soak the pretreated glass slide in a mixed solution containing 1% Nafion and 10 mg / L BBF, take it out after 12 hours, and store it after air drying;

[0036] (3) figure 2 It is the atomic force microscope picture of the antibacterial coating prepared in the present embodiment. The antibacterial coating prepared by the present embodiment is tested for antibacterial performance, and the mixed bacteria suspension prepared from the activated sludge of the sewage treatment plant is used as the experimental object, and the results are as follows: image 3 As shown, after the biofilm was cultivated for 6 hours, the antibacterial coating had a 43% inhibitory rate to the mixed bacteria biofilm; when the culture time was extended to 12-72h, the antibacterial coating still had a s...

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Abstract

The invention discloses a method for preparing a Nafion-bromofuranone composite antimicrobial coating on a substrate surface, which is characterized by comprising the following steps: (1) pretreating the substrate; and (2) preparing the Nafion-bromofuranone composite antimicrobial coating on the substrate surface. The method has is simple to operate, has the advantages of high applicability, low cost, no pollution and the like, is a biomembrane control method on the basis of non-bactericidal mechanism, and solves the problem that the traditional antimicrobial agent can enhance the drug resistance of bacteria; and the antimicrobial coating has favorable antimicrobial effects on mixed bacterium biomembranes and pure bacterium biomembranes.

Description

technical field [0001] The invention relates to a method for preparing a Nafion-bromofuranone composite antibacterial coating on the surface of a substrate, and belongs to the technical field of biofilm control. Background technique [0002] A biofilm is a structured microbial community composed of microorganisms attached to the surface of an inert or active entity and a hydrated matrix enclosing the microorganisms. Bacteria within biofilms bind cells together through their own secreted extracellular polymeric substances (EPS), forming complex microbial cell communities. The formation of harmful biofilms can cause many problems. In the medical field, approximately 80% of human bacterial infections are caused by biofilms. In the field of environment, the formation of biofilm on the surface of membrane bioreactor will increase the transmembrane pressure and cause the deterioration of effluent water quality, which seriously hinders the wide application of membrane technology....

Claims

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Application Information

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IPC IPC(8): C03C17/28C23C26/00
CPCC03C17/28C03C2218/111C23C26/00
Inventor 全向春武亚川王昕睿
Owner BEIJING NORMAL UNIVERSITY