PAP (pokeweed antiviral protein) freeze-dried powder compounding agent and preparation method of thereof

An anti-virus and protein freezing technology, applied in the field of medicine, can solve the problems of volatile inactivation, affecting the anti-viral function of drugs, etc., and achieve the effects of treating HPV virus infection, good clinical application prospects, and easy storage.

Active Publication Date: 2015-02-18
海南森瑞谱生命科学药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although pokeweed antiviral protein has antiviral function, it is easily denatured a

Method used

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  • PAP (pokeweed antiviral protein) freeze-dried powder compounding agent and preparation method of thereof
  • PAP (pokeweed antiviral protein) freeze-dried powder compounding agent and preparation method of thereof
  • PAP (pokeweed antiviral protein) freeze-dried powder compounding agent and preparation method of thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] 1. Preparation of natural pokeweed antiviral protein

[0073] (1) Pretreatment: grind pokeweed plant and protein extract in a ratio of 1:4 (w / v) to grind and homogenate, filter, adjust the pH of the supernatant to 3-6, and overnight at 4°C;

[0074](2) Rough extraction: centrifuge to get the supernatant, add solid ammonium sulfate to a final concentration of 40%, leave it overnight at room temperature, and carry out the first salting out; collect the supernatant by centrifugation, add solid ammonium sulfate to a final concentration of 70%, and place it at room temperature Overnight, carry out the second salting out; centrifuge to get the precipitate, which is PAP crude protein, store it at -20°C, and set aside;

[0075] (3) Purification: PAP crude protein is dissolved in phosphate buffer, gel filtration, ion exchange column chromatography and ultrafiltration technology are used to purify pokeweed antiviral protein, concentrated and vacuum freeze-dried.

[0076] 2. Prep...

Embodiment 2

[0087] 1. Preparation of natural pokeweed antiviral protein

[0088] (1) Pretreatment: grind pokeweed plant and protein extract in a ratio of 1:5 (w / v) to grind and homogenate, filter, adjust the supernatant to PH3-6, and overnight at 4°C;

[0089] (2) Rough extraction: centrifuge to get the supernatant, add solid ammonium sulfate to a final concentration of 45%, leave it overnight at room temperature, and carry out the first salting out; collect the supernatant by centrifugation, add solid ammonium sulfate to a final concentration of 75%, and place it at room temperature Overnight, carry out the second salting out; centrifuge to get the precipitate, which is PAP crude protein, store it at -20°C, and set aside;

[0090] (3) Purification: PAP crude protein is dissolved in phosphate buffer, gel filtration, ion exchange column chromatography and ultrafiltration technology are used to purify pokeweed antiviral protein, concentrated and vacuum freeze-dried.

[0091] 2. Preparation...

Embodiment 3

[0102] 1. Preparation of natural pokeweed antiviral protein

[0103] (1) Pretreatment: grind pokeweed plant and protein extract in a ratio of 1:3 (w / v) to grind and homogenate, filter, adjust the supernatant to PH3-6, and overnight at 4°C;

[0104] (2) Rough extraction: centrifuge to get the supernatant, add solid ammonium sulfate to a final concentration of 55%, leave it overnight at room temperature, and carry out the first salting out; collect the supernatant by centrifugation, add solid ammonium sulfate to a final concentration of 85%, and place it at room temperature Overnight, carry out the second salting out; centrifuge to get the precipitate, which is PAP crude protein, store it at -20°C, and set aside;

[0105] (3) Purification: PAP crude protein is dissolved in phosphate buffer, gel filtration, ion exchange column chromatography and ultrafiltration technology are used to purify pokeweed antiviral protein, concentrated and vacuum freeze-dried.

[0106] 2. Preparation...

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Abstract

The invention discloses a PAP (pokeweed antiviral protein) freeze-dried powder compounding agent as well as a preparation method and an application thereof, in particular to an application in treatment and prevention of HPV (human papillomavirus) virus infection and related diseases. The PAP freeze-dried powder compounding agent comprises PAP freeze-dried powder and a dissolution liquid, wherein components of the PAP freeze-dried powder comprise PAP, phyto-laccasaponin, polysaccharide and cordycepin; and components of the dissolution liquid comprise water, ethanol, phosphate, glycerol, menthol and preservative. The compounding agent prepared by taking PAP as a main component can effectively treat HPV infection, prevent cervical cancer and penis cancer, has a remarkable curative effect on diseases such as CIN (cervical intraepithelial neoplasia), cervical erosion, cervical polyp, cervical hypertrophy and like caused by HPV infection, and has good clinic application prospect.

Description

technical field [0001] The invention relates to the field of medicine, more specifically, relates to pokeweed antiviral protein freeze-dried powder compound and its preparation method, as well as its application in the treatment and prevention of HPV infection and related diseases. Background technique [0002] 1. HPV virus morphology, gene map and function [0003] Human papillomavirus (Human Papillomavirus, HPV) was discovered by Strauss under the electron microscope in 1949. Virus particles are spherical, 20-hedral symmetry, diameter 45-55nm, small DNA virus with double-stranded closed loop, no viral envelope, about 8000 base pairs, is an epitheliophilic virus, widely distributed in humans and animals , with a high degree of specificity. Most HPV virus genomes contain 8 open reading frames, which are divided into 3 regions according to their functions: [0004] (1) Non-coding regulatory region (about 400-1000bp), whose function is to regulate virus replication and gene...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K9/14A61K47/36A61K47/26A61P31/20A61P35/00A61P15/00A61K31/7076A61K31/715
Inventor 张春发邓柳红黄超王桂玉王胜高海波陈一萍
Owner 海南森瑞谱生命科学药业股份有限公司
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