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Indole terpene speradine B derived from aspergillus oryzae and application

A kind of Aspergillus oryzae, application technology, applied in indoleterpene speradine B and its application field, can solve the problems such as no drugs have been seen, and achieve the effect of significant anti-tumor activity

Inactive Publication Date: 2015-02-25
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity, and there is no report on the anti-proliferation activity of the compound on Hela cells, so there is no drug related to it in the market

Method used

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  • Indole terpene speradine B derived from aspergillus oryzae and application
  • Indole terpene speradine B derived from aspergillus oryzae and application
  • Indole terpene speradine B derived from aspergillus oryzae and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Fermentative production and separation and purification of the compound of embodiment 1

[0019] 1 Fermentation production

[0020] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, get Aspergillus oryzae ( Aspergillus oryzae ) IBPT-1 (preserved in China Center for Type Culture Collection on December 1, 2011, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2011437). Appropriate amount, inoculated on PDA solid slant medium and cultivated in 28°C incubator for 4 days.

[0021] Aspergillus oryzae ( Aspergillus oryzae ) appropriate amount of IBPT-1, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, constant volume of seawater] in a 1000mL Erlenmeyer flask, after static culture at 28°C for 30 days, the mycelia and fermentation broth were obtained.

[00...

Embodiment 2

[0029] Example 2 Test of anti-tumor activity in vitro

[0030] 1 Experimental samples and experimental methods

[0031] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0032] Cell lines and subculture of cells Tumor cell lines were used, and tumor cells were subcultured in DMEM medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0033] Cell Proliferation Inhibitory Activity Test Method

[0034] Lissamine rhodamine B (SRB) method Take Hela cells in the logarithmic growth phase and prepare them with fresh RPMI-1640 medium to a density of 2×10 per ml 5The cell suspension of each cell was inoculated in a 96-well cell culture plate at 200 microliters per well, and 2 microliters of different concen...

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PUM

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Abstract

The invention relates to an indole terpene speradine B derived from aspergillus oryzae and application. The invention relates to an indole terpene speradine H derived from aspergillus oryzae and application. The structural formula of the compound is shown in the specification. A fermentation product is acquired by performing fermentation culturing on aspergillusoryzae IBPT-1, and then the compound is obtained by performing separation purification on the fermentation product. Experiments confirm that the compound has relatively antitumor activity on human cervical cancer cell line hela, and is applicable to antitumor research by being used to prepare medicines inhibiting cell proliferation or antitumor medicines.

Description

technical field [0001] The invention relates to an indoterpene speradine B derived from Aspergillus oryzae and its application. Background technique [0002] Indoterpenes are a class of compounds with unique biological activity isolated from the secondary metabolites of fungi and bacteria. They have inhibitory activity on potassium ion channels, antitumor activity, selective and potent progesterone receptor competitive Activity of methicillin-resistant Staphylococcus aureus. Cyclopiazonic acid (CPA) alkaloids are an important branch of indoterpenoids, they usually contain three structural units: an indole ring, a hemiterpene and two acetic acid branches. So far, only 6 similar natural products have been found. [0003] The inventors have learned from research that Aspergillus oryzae (Aspergillus oryzae) IBPT-1, (has been preserved in the China Center for Type Culture Collection on December 1, 2011, address: Wuhan University, Wuhan, and the preservation number is: CCTCC NO:...

Claims

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Application Information

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IPC IPC(8): C07D487/06C12P17/18A61P35/00C12R1/69
CPCC07D487/06C12P17/182
Inventor 张其清陈立胡筱王丙帅
Owner FUZHOU UNIVERSITY