Extraction method of micromolecular sheep placenta extract and application of micromolecular sheep placenta extract in field of skin-care products

An extraction method and technology of sheep placenta, applied in cosmetics, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as limiting the use range and effect of sheep placenta, to ensure maximum utilization, maintain natural activity, and increase use rate Effect

Inactive Publication Date: 2015-03-04
SHANGHAI TAO TAO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some even add a large amount of trypsin or pepsin-digested protein peptides to the purer sheep placenta extracted, and these peptides are very complex mixtures, which limit the scope of use of

Method used

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  • Extraction method of micromolecular sheep placenta extract and application of micromolecular sheep placenta extract in field of skin-care products
  • Extraction method of micromolecular sheep placenta extract and application of micromolecular sheep placenta extract in field of skin-care products
  • Extraction method of micromolecular sheep placenta extract and application of micromolecular sheep placenta extract in field of skin-care products

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for extracting small molecule sheep placenta, carried out according to the following steps:

[0046] (1) Choose healthy, 2-3-year-old multiparous ewes with normal estrus cycle as the sheep to be pregnant. All the sheep have been treated with quarantine, epidemic prevention and medicinal baths, and the feed is supplemented with green fodder, vitamins and trace amounts. element;

[0047] (2) Intramuscularly inject cloprostenol at 7-14 days of the luteal phase of the expectant sheep, and start to observe estrus 30 hours later. When estrus is observed, artificial semen collection and insemination are performed 12 hours after estrus, and can be compounded once every 24 days after estrus. After 35 days, a B-ultrasound examination was performed to confirm the pregnancy status;

[0048] (3) When the fetal sheep grows to 85-100 days, the fetal sheep is taken out by operation, placed in a high-temperature sterilized container, washed with sterile saline, and the eyeball...

Embodiment 2

[0062] (1) The ewes were dissected by surgical methods to obtain sterile fetal sheep, which were placed in sterile containers, washed with normal saline, and eyeballs, brain, liver, lungs, heart, intestines and stomach were removed.

[0063] (2) Rinse with sterile saline, drain and weigh.

[0064] (3) Add sterile pure water and fragments of fetal sheep to homogenate, add pure water to dilute the homogenate, the quality of pure water is 3 times that of fetal sheep, and the homogenate dilution is filtered through a three-layer stainless steel filter screen, and the upper layer is 20 Mesh, the middle layer is 50 mesh, and the lower layer is 100 mesh.

[0065] (4) The homogenized filtrate is placed in a sterile container and placed in a -30°C refrigerator, frozen overnight, or stored.

[0066] (5) The frozen homogenate filtrate was taken out and thawed at room temperature, transferred to a sterile heat-resistant container, placed in a 70°C water bath, and kept at 70°C for 30 minu...

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Abstract

The invention discloses an extraction method of a micromolecular sheep placenta extract and application of the micromolecular sheep placenta extract in the field of skin-care products, belonging to the technical field of biology. The extraction method disclosed by the invention comprises the following steps of obtaining a lot of sheep pregnant in the same period by adopting synchronization of estrus, taking out sheep fetus after 85-100 days through an operation method to obtain body homogenate, carrying out repetitive freeze-thawing and splitting cells, degenerating at high temperature, sterilizing, precipitating hybrid protein, carrying out 30 KD ultrafiltration after centrifuging at high speed so as to remove toxic and harmful components and impurities, such as macromolecular hybrid protein, bacteria and endogenous virus, so as to obtain a pure micromolecular sheep placenta extract product, and carrying out ultrafiltration and concentration of the micromolecular sheep placenta extract product by using a 1 KD ultrafiltration component. The micromolecular sheep placenta extract disclosed by the invention can be widely used in the fields of skin-care products, beauty products, wound healing products, health-care products, scientific searches and the like; the extraction method disclosed by the invention provides a novel important way for producing the sheep placenta extract; furthermore, the compositions of the micromolecular sheep placenta extract are authenticated for the first time, and thus, theoretical foundation is provided for scientifically using the sheep placenta extract.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting small-molecule sheep placenta and its application in the field of skin care products. Background technique [0002] Sheep placenta contains two different products, the Extract of Sheep`s Placental and the Extract of Goat`s Embryo. [0003] Sheep placenta is a biologically active substance in sheep placenta, which is widely used in various cosmetics, medicines and health foods after extraction. [0004] Sheep placenta exists in fetal sheep, and after extraction, it is widely used in various cosmetics, medicines and health foods. [0005] Extracting active substances from sheep embryos is much more difficult and precious than extracting active substances from placenta, and the composition of active substances is also quite different. Sheep placenta can activate human aging cells, promote cell regeneration and division. [0006] In 1912, Swiss scie...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/02A61Q19/08A61Q19/00
Inventor 曾凡一黄淑帧
Owner SHANGHAI TAO TAO ENG
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