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Strong-stability lactic acid detection reagent

A technology for lactic acid detection and strong stability, which is applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of being easily interfered by other components, low analytical sensitivity, and serious interference reactions, etc., and achieves improvement. High detection accuracy and sensitivity, good detection effect and low cost

Active Publication Date: 2015-03-04
孟怡然 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the three methods of YSI blood lactate analyzer test method, spectrophotometry and colorimetry are not very sensitive and are easily interfered by other components, so now hospitals and scientific research institutions mostly use enzyme method for detection. High analytical sensitivity, combined with automatic biochemical analysis instruments, easy to operate, reasonable cost, and mostly accepted by people
However, some enzymes need to be added to the liquid reagents to participate in the reaction, and the added peroxidase has poor specificity to the substrate, seriously interferes with the reaction, and has poor analysis accuracy.
In addition, the lactate oxidase in the reagent is unstable in a liquid state and is easily inactivated at high temperatures. If the amount of lactate oxidase is increased, the cost will inevitably increase, which will bring great harm to the promotion of the product. influences

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A highly stable lactic acid detection reagent, which includes reagent R1 and reagent R2; each component in the reagent R1 is:

[0061] Tris buffer (pH=7.4) 100mmol / L

[0062] EDTA.Na 2 10mmol / L

[0063] Manganese acetate (Ⅲ) 10μmol / L

[0064] Peroxidase 2KU / L

[0065] Ascorbate enzyme 2KU / L

[0066] p-Hydroxybenzoic acid 1mol / L

[0067] Sodium chloride 2.5g / L

[0068] Sodium azide 1g / L

[0069] 4-aminoantipyrine 0.25mmol / L;

[0070] Each component of described reagent R2 is:

[0071] PIPES (1,4-piperazine diethanesulfonic acid) buffer (pH=7.4) 50mmol / L

[0072] EDTA.Na 2 10mmol / L

[0073] Nanoparticles 5mmol / L

[0074] Fatty alcohol polyoxyethylene ether 1g / L

[0075] Gelatin 1g / L

[0076] Lactate oxidase D 2KU / L

[0077] Sodium chloride 2.5g / L

[0078] Sodium azide 1g / L.

[0079] The above-mentioned nanoparticles are γ-Fe with a particle size of 5-20nm synthesized by conventional microe...

Embodiment 2

[0081] A highly stable lactic acid detection reagent, which includes reagent R1 and reagent R2; each component in the reagent R1 is:

[0082] Tris buffer (pH=7.4) 100mmol / L

[0083] EDTA.Na 2 100mmol / L

[0084] Manganese acetate (Ⅲ) 100μmol / L

[0085] Peroxidase 20KU / L

[0086] Ascorbate enzyme 8KU / L

[0087] p-Hydroxybenzoic acid 1mol / L

[0088] Sodium chloride 10g / L

[0089] Sodium azide 5g / L

[0090] 4-aminoantipyrine 0.25mmol / L;

[0091] Each component of described reagent R2 is:

[0092] PIPES (1,4-piperazine diethanesulfonic acid) buffer (pH=7.4) 50mmol / L

[0093] EDTA.Na 2 100mmol / L

[0094] Nanoparticles 30mmol / L

[0095] Fatty alcohol polyoxyethylene ether 10g / L

[0096] Gelatin 10g / L

[0097] Lactate oxidase D 8KU / L

[0098] Sodium chloride 10 g / L

[0099] Sodium azide 5 g / L.

[0100] The above-mentioned nanoparticles are γ-Fe with a particle size of 20-30nm synthesized by a convention...

Embodiment 3

[0102] A highly stable lactic acid detection reagent, which includes reagent R1 and reagent R2; each component in the reagent R1 is:

[0103] Tris buffer (pH=7.4) 100mmol / L

[0104] EDTA.Na 2 50mmol / L

[0105] Manganese acetate (Ⅲ) 60μmol / L

[0106] Peroxidase 10KU / L

[0107] Ascorbate enzyme 6KU / L

[0108] p-Hydroxybenzoic acid 1mol / L

[0109] Sodium chloride 6g / L

[0110] Sodium azide 3g / L

[0111] 4-aminoantipyrine 0.25mmol / L.

[0112] Each component in the reagent R2 is:

[0113]1,4-piperazine diethanesulfonic acid buffer (pH=7.4) 50mmol / L

[0114] EDTA.Na 2 50mmol / L

[0115] Nanoparticles 20mmol / L

[0116] Fatty alcohol polyoxyethylene ether 6g / L

[0117] Gelatin 6g / L

[0118] Lactate oxidase D 6KU / L

[0119] Sodium chloride 8 g / L

[0120] Sodium azide 3 g / L.

[0121] The above-mentioned nanoparticles are γ-Fe with a particle size of 10-20nm synthesized by a conventional ultrasonic precipit...

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Abstract

The invention discloses a strong-stability lactic acid detection reagent, belonging to the technical field of clinical in-vitro detection. The strong-stability lactic acid detection reagent comprises two components, namely a reagent R1 and a reagent R2; substances in the reagent R1 and the reagent R2 are reasonably matched in use, so that the whole reagent system is in a stable state. Compared with a conventional lactic acid detection reagent, the strong-stability lactic acid detection reagent has a relatively excellent stability effect and is beneficial to the clinical popularization and use.

Description

technical field [0001] The invention relates to a highly stable lactic acid detection reagent, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Blood lactic acid (LAC) is an intermediate product of glucose metabolism in the body, mainly produced by red blood cells, striated muscle and brain tissue. The concentration of lactic acid in the blood mainly depends on the synthesis speed and metabolic rate of the liver and kidney. In certain pathological conditions (such as respiratory failure or circulatory failure), tissue hypoxia can be caused, and lactic acid in the body can increase due to hypoxia. In addition, in the process of glucose metabolism in the body, such as increased glycolysis speed, strenuous exercise, and dehydration, it can also cause increased lactic acid in the body, and increased lactic acid in the body can cause lactic acidosis. [0003] Lactic acidosis is divided into two types, A and B: Type A (clinical ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28C12Q1/26C12Q1/25
Inventor 甘宜梧罗维晓谭柏清王绮李静肖慧朱玉娥
Owner 孟怡然
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