A highly stable lactic acid detection reagent
A lactic acid detection technology with strong stability, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of being easily interfered by other components, the analytical sensitivity is not very high, and the substrate specificity is poor. Achieve the effect of improving detection accuracy and sensitivity, good detection effect, and not easy to decompose
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Embodiment 1
[0059] A highly stable lactic acid detection reagent, which includes reagent R1 and reagent R2; each component in the reagent R1 is:
[0060] Tris buffer (pH=7.4) 100mmol / L
[0061] EDTA.Na 2 10mmol / L
[0062] C 6 h 13 MnO 8 10μmol / L
[0063] Peroxidase 2KU / L
[0064] Ascorbate enzyme 2KU / L
[0065] p-Hydroxybenzoic acid 1mol / L
[0066] Sodium chloride 2.5g / L
[0067] Sodium azide 1g / L
[0068] 4-aminoantipyrine 0.25mmol / L;
[0069] Each component of described reagent R2 is:
[0070] PIPES (1,4-piperazine diethanesulfonic acid) buffer (pH=7.4) 50mmol / L
[0071] EDTA.Na 2 10mmol / L
[0072] Nanoparticles 5mmol / L
[0073] Fatty alcohol polyoxyethylene ether 1g / L
[0074] Gelatin 1g / L
[0075] Lactate oxidase D 2KU / L
[0076] Sodium chloride 2.5g / L
[0077] Sodium azide 1g / L.
[0078] The above-mentioned nanoparticles are γ-Fe with a particle size of 5-20nm synthesized by conventional microemulsion-hydrothermal method 2 o 3 Nanoparticles.
Embodiment 2
[0080] A highly stable lactic acid detection reagent, which includes reagent R1 and reagent R2; each component in the reagent R1 is:
[0081] Tris buffer (pH=7.4) 100mmol / L
[0082] EDTA.Na 2 100mmol / L
[0083] C 6 h 13 MnO 8 100μmol / L
[0084] Peroxidase 20KU / L
[0085] Ascorbate enzyme 8KU / L
[0086] p-Hydroxybenzoic acid 1mol / L
[0087] Sodium chloride 10g / L
[0088] Sodium azide 5g / L
[0089] 4-aminoantipyrine 0.25mmol / L;
[0090] Each component of described reagent R2 is:
[0091] PIPES (1,4-piperazine diethanesulfonic acid) buffer (pH=7.4) 50mmol / L
[0092] EDTA.Na 2 100mmol / L
[0093] Nanoparticles 30mmol / L
[0094] Fatty alcohol polyoxyethylene ether 10g / L
[0095] Gelatin 10g / L
[0096] Lactate oxidase D 8KU / L
[0097] Sodium chloride 10 g / L
[0098] Sodium azide 5 g / L.
[0099] The above-mentioned nanoparticles are γ-Fe with a particle size of 20-30nm synthesized by a conventional ultrasonic precipitation method. 2 o 3 Nanoparticles.
Embodiment 3
[0101] A highly stable lactic acid detection reagent, which includes reagent R1 and reagent R2; each component in the reagent R1 is:
[0102] Tris buffer (pH=7.4) 100mmol / L
[0103] EDTA.Na 2 50mmol / L
[0104] C 6 h 13 MnO 8 60μmol / L
[0105] Peroxidase 10KU / L
[0106] Ascorbate enzyme 6KU / L
[0107] p-Hydroxybenzoic acid 1mol / L
[0108] Sodium chloride 6g / L
[0109] Sodium azide 3g / L
[0110] 4-aminoantipyrine 0.25mmol / L.
[0111] Each component in the reagent R2 is:
[0112] 1,4-piperazine diethanesulfonic acid buffer (pH=7.4) 50mmol / L
[0113] EDTA.Na 2 50mmol / L
[0114] Nanoparticles 20mmol / L
[0115] Fatty alcohol polyoxyethylene ether 6g / L
[0116] Gelatin 6g / L
[0117] Lactate oxidase D 6KU / L
[0118] Sodium chloride 8 g / L
[0119] Sodium azide 3 g / L.
[0120] The above-mentioned nanoparticles are γ-Fe with a particle size of 10-20nm synthesized by a conventional ultrasonic precipitation method. 2 o 3 Nanoparticles.
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