Dual-wavelength enzyme-immunization chemiluminescent substrate and application thereof

A chemiluminescence and chemiluminescence enzyme technology, applied in scientific instruments, measuring devices, instruments, etc., can solve problems such as high price, and achieve the effects of long storage time, convenient use and simple preparation method

Active Publication Date: 2015-03-04
天津华信康达科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the chemiluminescence immunoassay instruments and diagnostic reagents used in clinical medicine in our country basically come from abroad, such as Roche in Germany, Beckman in the United States, Siemens in Germany, Solo in Germany, etc. Lin (Liaison), Abbott (Abbott) in the United States, Tosoh (AIA) in Japan, etc., although the substrates of chemiluminescent immunoassay diagnostic reagents are different, their prices are relatively expensive, especially those of Tosoh (AIA) in Japan. A set of matrix solution (substrate) for chemiluminescent enzyme (alkaline phosphatase) immunoassay diagnostic reagents (200ml) costs about 3,000 yuan, and the matrix solution is a dual-wavelength substrate (patent confidentiality)

Method used

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  • Dual-wavelength enzyme-immunization chemiluminescent substrate and application thereof
  • Dual-wavelength enzyme-immunization chemiluminescent substrate and application thereof
  • Dual-wavelength enzyme-immunization chemiluminescent substrate and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of the dual-wavelength immunochemical luminescence substrate of alkaline phosphatase in this embodiment is carried out according to the following steps:

[0027] 1. Preparation of component A: Mix 5 g of mannitol (dry excipient), 60 mg of 4-methylumbelliferone phosphate (4-MUP) and 10 ml of carbonate buffer solution of 0.01mol / L pH 9.5, Carry out vacuum freeze-drying, store at 4 ℃ after drying;

[0028] 2. Preparation of component B: 4.5ml of 2-amino-2-methyl-1-propanol, 30mg of 5-hydroxytryptamine (Serotonin), MgCl 2 .6H 2 O 20mg, NaN3 100mg and 0.01mol / L, PH 9.5 carbonate buffer 100ml, mix until completely dissolved, and set aside.

Embodiment 2

[0030] The method for using the dual-wavelength immunochemiluminescent substrate of this embodiment is carried out in the following steps:

[0031] Add the B component of the present invention directly to the A component, and after mixing evenly, replace the Japanese Tosoh (AIA) matrix solution (substrate). On the matrix liquid position of the analyzer, the detection steps are carried out according to the instructions of the Japan AIA chemiluminescence enzyme immunoassay analyzer.

[0032] Specifically include the following steps:

[0033] 1. At room temperature, add the component B in Example 1 directly into the bottle containing component A, mix it evenly to form the matrix solution, and let it stand for 3-5 minutes at room temperature;

[0034] 2. Place the liquid prepared in step 1 on the substrate liquid position of Tosoh AIA chemiluminescent enzyme immunoassay analyzer;

[0035] 3. Put the sample to be tested and the diagnostic reagent on the designated position of the...

Embodiment 3

[0039] In this example, the dual-wavelength immunochemiluminescent substrate in Example 1 is used to detect serum estradiol (E2) using a fluorescence photometer, and the operation steps are as follows: figure 1 shown.

[0040] Verification test 1

[0041] Comparison of standard curves:

[0042] On the Japanese AIA chemiluminescence immunoassay analyzer (model 360), use AIA matrix solution (substrate solution) and the present invention dual-wavelength immunochemiluminescence substrate solution respectively, carry out the preparation of E2 standard curve simultaneously, and carry out the mathematics of correlation coefficient Statistics, the results are shown in Table 1.

[0043] Table 1AIA matrix solution (substrate solution) and embodiment dual-wavelength substrate solution prepare the comparison of E2 standard curve

[0044]

[0045] The concentration value of E2 and the relative fluorescence value of the AIA matrix solution and the substrate solution of the present inven...

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Abstract

The invention discloses a dual-wavelength enzyme-immunization chemiluminescent substrate and application thereof, and particularly relates to application for testing or analyzing composition of materials by utilizing enzyme-immunization chemiluminescence. The dual-wavelength enzyme-immunization chemiluminescent substrate provided by the invention has the advantages of simple preparation method, convenience in use, high accuracy and precision of sample detection and long storage time. The enzyme-immunization chemiluminescent substrate is simple in preparation and convenient in use; the prepared luminescent substrate not only can guarantee high accuracy, precision and repeatability of detection data, but also is low in cost. The dual-wavelength enzyme-immunization chemiluminescent substrate is applied to the field of enzyme-immunization chemiluminescence analysis.

Description

technical field [0001] The invention relates to a composition for testing or analyzing materials by using chemiluminescence and its application, in particular to a dual-wavelength immunochemiluminescent substrate and its application. Background technique [0002] Chemiluminescence immunoassay (CLIA) is an analytical method that combines highly sensitive chemiluminescent assay technology with highly specific immune responses. It is used for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, The detection and analysis of vitamins and drugs is also a newest immunoassay technology developed after radioimmunoassay, enzyme-linked immunoassay, and time-resolved fluorescent immunoassay, and is widely used in medical clinical testing and other fields. [0003] Chemiluminescence immunoassay consists of two parts, namely immune reaction system and chemiluminescence analysis system. The immune response system is to directly label luminescent substances or enzymes on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 刘军田恩江张静李小永
Owner 天津华信康达科技有限公司
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