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Method for preparing compound amino acid through extensive enzymatic hydrolysis of soybean protein

A technology for compound amino acids and soybean protein, which is applied in the field of compound amino acid preparation by in-depth enzymatic hydrolysis of soybean protein, and achieves the effects of improving the sensitivity of enzymatic hydrolysis, improving the yield and improving the degree of degradation.

Active Publication Date: 2015-03-11
广州合诚实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no enzymatically hydrolyzed plant protein source compound amino acid product in the domestic market

Method used

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  • Method for preparing compound amino acid through extensive enzymatic hydrolysis of soybean protein
  • Method for preparing compound amino acid through extensive enzymatic hydrolysis of soybean protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The operation process is as figure 1 Shown:

[0044] (1) Raw material moisture adjustment: add deionized water to 100kg soybean protein isolate raw material (on a dry basis, the protein content is 92.6%), stir and disperse evenly, so that the water content is 20% (w / w);

[0045] (2) Extrusion pretreatment: use a twin-screw extruder with an extrusion temperature of 100°C, a die hole diameter of 20mm, and a screw speed of 110r / min to extrude soybean protein. After extrusion, use a high-speed pulverizer to crush The extruded soybean protein is crushed to 60 mesh;

[0046] (3) Dispersion and hydration of soybean protein: the soybean protein powder after pulverization is prepared as a soybean protein solution with a mass percentage of 5% with deionized water, and the soybean protein solution is dispersed and hydrated (shear speed is 2000rpm, time 15min), the temperature was raised to 50°C, and the pH value was adjusted to 8.5 with sodium hydroxide solution;

[0047] (4) S...

Embodiment 2

[0050] (1) Raw material moisture adjustment: add deionized water to 100kg of soybean protein isolate raw material (on a dry basis, with a protein content of 92.6%), stir and disperse evenly, so that the water content is 25% (w / w);

[0051] (2) Extrusion pretreatment: use a twin-screw extruder with an extrusion temperature of 95°C, a die hole diameter of 15mm, and a screw speed of 100r / min to extrude soybean protein. After extrusion, use a high-speed pulverizer to crush The extruded soybean protein is crushed to 40 mesh;

[0052] (3) Dispersion and hydration of soybean protein: the soybean protein powder after crushing is prepared as 8% soybean protein solution by mass percentage with deionized water, and the soybean protein solution is dispersed and hydrated (shear speed is 3000rpm, time 10min), the temperature was raised to 45°C, and the pH value was adjusted to 8.0 with sodium hydroxide solution;

[0053] (4) Soybean protease membrane reaction based on ultrafiltration separ...

Embodiment 3

[0056] (1) Raw material moisture adjustment: add deionized water to 100 kg of soybean protein isolate raw material (on a dry basis, with a protein content of 92.6%), stir and disperse evenly, so that the water content is 30% (w / w);

[0057] (2) Extrusion pretreatment: use a twin-screw extruder with an extrusion temperature of 105°C, a die hole diameter of 25mm, and a screw speed of 120r / min to extrude soybean protein. After extrusion, use a high-speed pulverizer to crush The extruded soybean protein is crushed to 80 mesh;

[0058] (3) Dispersion and hydration of soybean protein: the soybean protein powder after crushing is prepared as a 10% soybean protein solution by mass percentage with deionized water, and the soybean protein solution is dispersed and hydrated (shear speed is 2500rpm, time 15min), the temperature was raised to 55°C, and the pH value was adjusted to 8.5 with sodium hydroxide solution;

[0059] (4) Soybean protease membrane reaction based on ultrafiltration ...

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Abstract

The invention discloses a method for preparing compound amino acid through extensive enzymatic hydrolysis of soybean protein. The method comprises the following steps: carrying out extrusion treatment on soybean isolate protein filled with water through a screw rod expansion extruder and crushing the extruded soybean protein; preparing the crushed soybean protein powder into a soybean protein solution by using water, dispersing and hydrating; raising the temperature to 45-55 DEG C, regulating a pH value to 8-9, adding alkaline protease, stirring at a constant temperature of 45-55 DEG C for enzymatic hydrolysis till the pH value of the system is 7-7.5, adding neutral proteinase and flavored proteinase, starting a secondary ultra-filtration system to carry out enzyme membrane reaction and pumping trapped fluid of a first stage of ultra-filtration membrane and trapped fluid of a second stage of ultra-filtration membrane to a reaction tank for reuse; when the size of a reaction substrate is reduced to 1 / 4, supplementing the same volume of water; concentrating, sterilizing, atomizing and drying a permeate of the second stage of ultra-filtration membrane to obtain the compound amino acid powder. Through the method, the yield is high, and the content of free amino acid in the obtained compound amino acid accounts for more than 40 percent of the total amino acid content.

Description

technical field [0001] The invention belongs to the technical field of protein deep processing and utilization, and in particular relates to a method for preparing complex amino acids by deep enzymolysis of soybean protein. Background technique [0002] Amino acid is the most basic substance that constitutes the protein of organisms and is related to life activities. It is the basic unit of protein and has a close relationship with the life activities of organisms. It has special physiological functions in antibodies and is indispensable in organisms. one of the nutrients. There are more than 20 amino acids that make up the human body, which are divided into two types: essential amino acids and non-essential amino acids. Among them, "non-essential amino acids" can be synthesized by the human body without food or drug supplements, "essential amino acids" (including isoleucine, leucine, lysine, methionine, tryptophan, threonine, phenylalanine and valine) the human body canno...

Claims

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Application Information

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IPC IPC(8): A23J3/16A23J3/26A23J3/34
Inventor 周雪松曾建新蒋文真
Owner 广州合诚实业有限公司
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